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Plasmid#221978PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 DdCBE C2
Plasmid#221979PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 DdCBE T1
Plasmid#221980PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 DdCBE T2
Plasmid#221981PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-T-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationPromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 alphaDdCBE G1
Plasmid#221982PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE G1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 alphaDdCBE C2
Plasmid#221983PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE C2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 alphaDdCBE T1
Plasmid#221984PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T1 repeats-TALE CTD-DddAtox 1397C-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
ND4 alphaDdCBE T2
Plasmid#221985PurposeExpresses mitochondrial cytosine base editor arm in mammalian cellsDepositorInsertCOX8A MTS-3xFLAG-TALE NT-alphaN-ND4 TALE T2 repeats-TALE CTD-DddAtox 1397N-UGI-ATP5B 3'UTR
UseCRISPRTags3xFLAGExpressionMammalianMutationTALE: Q231R, W232G, S233APromoterCMVAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX DdCBE DddA6 1397N
Plasmid#221934PurposeBackbone plasmid for FusX-based assembly of DdCBE arm with DddA6 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationDddA: Q1310R, S1330I, T1380IPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX DdCBE DddA11 1397N
Plasmid#221935PurposeBackbone plasmid for FusX-based assembly of DdCBE arm with DddA11 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationDddA: S1330I, A1341V, N1342S, E1370K, T1380IPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE 1397C
Plasmid#221936PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397CDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationTALE: Q231R, W232G, S233APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE 1397N
Plasmid#221937PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddAtox 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationTALE: Q231R, W232G, S233APromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA6-11 1397C
Plasmid#221938PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6/11 1397CDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationTALE: Q231R, W232G, S233A | DddA: T1413IPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA6 1397N
Plasmid#221939PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA6 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationTALE: Q231R, W232G, S233A | DddA: Q1310R, S1330I,…PromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX alphaDdCBE DddA11 1397N
Plasmid#221940PurposeBackbone plasmid for FusX-based assembly of alphaDdCBE arm with DddA11 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationTALE: Q231R, W232G, S233A | DddA: S1330I, A1341V,…PromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX DdCBE 1397C
Plasmid#221931PurposeBackbone plasmid for FusX-based assembly of DdCBE arm with DddAtox 1397CDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX DdCBE 1397N
Plasmid#221932PurposeBackbone plasmid for FusX-based assembly of DdCBE arm with DddAtox 1397NDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
FusX DdCBE DddA6-11 1397C
Plasmid#221933PurposeBackbone plasmid for FusX-based assembly of DdCBE arm with DddA6/11 1397CDepositorTypeEmpty backboneUseSynthetic BiologyTags3xFLAGExpressionMutationDddA: T1413IPromoterAvailable sinceSept. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
Luciferase shRNA
Plasmid#225343PurposeshRNAmir backbone for RNA interference, with YFPDepositorInsertLuciferase shRNA (LOC116160065 Mouse)
UseAAV and RNAiTagsExpressionMammalianMutationPromoterCBAAvailable sinceSept. 11, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM-TEV
Plasmid#222101PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.DepositorInsertBacteroidetes-bGSDM-TEV ORF
UseTags6xHis-SUMO2ExpressionBacterialMutationTEV protease site replaces residues E242–G248PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only