-
Plasmid#89633PurposeTargeted CpG methylationDepositorInsertsite-specific DNA-methyltransferase SssI
UseCRISPRTags3*FLAG and 6*HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-myc-OGA(GS-544)
Plasmid#193995Purposeplasmid encoding myc-tagged OGA(GS-544)DepositorInsertmyc-OGA(GS-544)
UseTagsmycExpressionMammalianMutationPromoterAvailable sinceJan. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Lats1 (Nigg HS189)
Plasmid#41156DepositorInsertLats1 (LATS1 Human)
UseTags3xMyc and PreScission SiteExpressionMammalianMutationPromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)_U6 tRNAPyl_CMV NESPylRS(AF)
Plasmid#182287PurposeEncodes codon-optimized AF mutant of M. mazei-derived pyrrolysine (Pyl) tRNA synthetase fused to a nuclear export signal (NESPylRSAF) & tRNACUAPyl used for amber codon suppression in mammalian cellsDepositorInsertscodon-optimized Y306A/Y384F (AF) double mutant of Methanosarcina mazei-derived pyrrolysine (Pyl) tRNA synthetase
PylT
UseTagsnuclear export signal (NES)ExpressionMammalianMutationY306A/Y384F (AF) double mutant of Methanosarcina …PromoterCMV and U6Available sinceMay 31, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.2 TDP-43 NLS1 YFP
Plasmid#84912PurposeMammalian expresion of TDP-43 NLS1 YFPDepositorInsertTDP-43 (TARDBP Human)
UseTagsYFPExpressionMammalianMutationK82A, R83A, K84APromoterCMVAvailable sinceNov. 23, 2016AvailabilityAcademic Institutions and Nonprofits only -
RGS-6xHis-BLIMP-1-pcDNA3.1-
Plasmid#52518Purposeexpresses human BLMP-1 in mammalian cells with RGS-6xHis tag at N-terminusDepositorInsertBlimp1 (PRDM1 Human)
UseTags6xHisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 31, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-ZNF598-TEV-3xFLAG
Plasmid#105690PurposeExpresses C terminally 3xFLAG tagged ZNF598 proteinDepositorInsertZNF598 (ZNF598 Human)
UseTagsTEV-3xFLAGExpressionMammalianMutationPromoterCMV (from vector)Available sinceFeb. 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 Flag p38 alpha
Plasmid#20351DepositorInsertpDCNA3 Flag p38 alpha (Mapk14 Mouse)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceApril 14, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 LIC cloning vector (6A)
Plasmid#30124DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceOct. 19, 2011AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 AIF 1-90 mCherry FRB
Plasmid#67530PurposeFor expression of mCherry fused FRB to the mitochondrial innermembraneDepositorInsertsAIF a.a. 1 - 90
mCherry
FRB
UseTagsAIF 1 - 90, FRB, and mCherryExpressionMammalianMutationT->LPromoterCMVAvailable sinceAug. 6, 2015AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-RhoA-Q63L
Plasmid#12968DepositorInsertRhoA constitutively active (RHOA Human)
UseTagsEGFPExpressionMammalianMutationQ63L Constitutively activePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5/FRT/TO-GAlphaQ-RLuc8
Plasmid#140982PurposeEncodes a G alpha subunit (GNAQ) containing RLuc8 as an optimal component of a BRET2 biosensor for studying heterotrimeric G protein dissociationDepositorInsertGAlphaQ-RLuc8 (GNAQ Human)
UseLuciferaseTagsExpressionMammalianMutationRLuc8 and flanking SGGGGS linkers have been inser…PromoterCMVAvailable sinceJune 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PB1-B/BRISBANE/60/2008
Plasmid#187919PurposeExpresses PB1 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase basic protein 1
UseTagsUNTAGGEDExpressionMutationPromoterCMV PromoterAvailable sinceOct. 17, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-PB2-B/BRISBANE/60/2008
Plasmid#187917PurposeExpresses PB2 subunit of Influenza B/Brisbane/60/2008 virus polymerase in mammalian cellsDepositorInsertpolymerase basic protein 2
UseTagsUNTAGGEDExpressionMutationPromoterCMV PromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-EGFP-RhoA-T19N
Plasmid#12967DepositorInsertRhoA DN (RHOA Human)
UseTagsEGFPExpressionMammalianMutationT19N Dominant negativePromoterAvailable sinceOct. 27, 2006AvailabilityAcademic Institutions and Nonprofits only -
RelA RHD cFlag pcDNA3
Plasmid#20015DepositorInsertRelA (Rela Mouse)
UseTagsFlagExpressionMammalianMutationContains only the Rel Homology Domain of RelA. It…PromoterAvailable sinceJan. 28, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-FLAG-human NPM1 wt
Plasmid#160692PurposeMammalian expression of FLAG-tagged NPM1DepositorInsertNPM1 (NPM1 Human)
UseTagsFLAGExpressionMammalianMutationPromoterAvailable sinceNov. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag-delN
Plasmid#112492PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationdeletion of N terminus (AA1-274 deleted)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-hRIPK1-Flag-delC
Plasmid#112493PurposeMammalian expression of the indicated Flag-hRIPK1 mutant. Please note Flag tag is N-terminal.DepositorInsertRIPK1 (RIPK1 Human)
UseTagsFlagExpressionMammalianMutationdeletion of C terminus (AA290-671 deleted)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1/Myc-His(-)-HSulf-2
Plasmid#13004DepositorInserthuman sulfatase 2 (SULF2 Human)
UseTags6x His and MycExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2006AvailabilityAcademic Institutions and Nonprofits only