Addgene

We narrowed to 5,242 results for: pCas

Showing: 3781 - 3800 of 5242 results

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pTX168

Plasmid

#89257

Purpose

Express STU (Single Transcriptional Unit) Cas9 with CaMV 35s promoter in plants

Depositor

Daniel Voytas

Article

Tang et al Mol Plant. 2016 Jul 6;9(7):1088-91. doi:

Type

Empty backbone

Use

CRISPR

Tags

SV40 NLS

Expression

Plant

Mutation

Promoter

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGJJ055

Plasmid

#183622

Purpose

ddPCA mutagenesis plasmid - Efficient mutant library cloning plasmid suitable for oligo synthesis, error prone PCR or nicking mutagenesis strategies.

Depositor

Ben Lehner

Article

Faure et al Nature. 2022 Apr;604(7904):175-183. doi:

Type

Empty backbone

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 10, 2022

Availability

Academic Institutions and Nonprofits only

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Guide-Generating Vector

Plasmid

#125752

Purpose

Guide-Generating Vector for creating the Cys4-guide-scaffold structure in the first round of assembly

Depositor

Rodrigo Ledesma-Amaro

Article

McCarty et al ACS Synth Biol. 2019 Apr 2. doi: 10.1021

Insert

Cys4-GFPdropout-SpCas9scaffold

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

July 10, 2019

Availability

Academic Institutions and Nonprofits only

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CAG::PSAMQ79G,Q139G:5HT3HC-IRES-GFP

Plasmid

#32474

Depositor

Scott Sternson

Article

Magnus et al Science. 2011 Sep 2;333(6047):1292-6.

Insert

PSAMQ79G,Q139G:5HT3 HC

Use

Tags

IRES-EGFP

Expression

Mammalian

Mutation

Promoter

CAG

Available since

April 10, 2012

Availability

Academic Institutions and Nonprofits only

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pZLSLD.R

Plasmid

#51511

Purpose

Single component LSL vector. Contains 2x35S:Zif268:FokI outside of the replicon borders. Within the replicon is the us:NPTII template.

Depositor

Daniel Voytas

Article

Baltes et al Plant Cell. 2014 Jan;26(1):151-63. doi:

Inserts

Zif268:FokI
us:NPTII repair template

Use

Plant t-dna plasmid

Tags

NLS

Expression

Mutation

Promoter

2x35S

Available since

May 2, 2014

Availability

Academic Institutions and Nonprofits only

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AL12R-EF-Tu

Plasmid

#161013

Purpose

PP7-tagged reporter driven by the Arabidopsis EF-Tu promoter

Depositor

Krishna Niyogi

Article

Alamos et al Nat Plants. 2021 Aug;7(8):1037-1049. doi

Insert

PP7 and H2B-mScarlet

Use

Tags

Expression

Plant

Mutation

Promoter

Available since

Dec. 11, 2020

Availability

Academic Institutions and Nonprofits only

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pFTK063

Plasmid

#171335

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m4) (for fusion)

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK062

Plasmid

#171334

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m2) (for fusion)

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK061

Plasmid

#171333

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

SpCas9 (for fusion)

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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pFTK057

Plasmid

#171329

Purpose

Part Plasmid Entry Vector (LVL0) from the Fungal Modular Cloning ToolKit.

Depositor

Arnold Driessen

Article

Mozsik et al ACS Synth Biol. 2021 Nov 2. doi: 10.1021

Insert

dSpCas9(m4)-VPR-NLS

Use

Synthetic Biology

Tags

Expression

Bacterial and Yeast

Mutation

n/a

Promoter

Available since

Jan. 3, 2022

Availability

Academic Institutions and Nonprofits only

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p169 pCMV/betaAc-EGFP/beta-gal

Plasmid

#8388

Depositor

Jeffrey Green

Article

Kaczmarczyk et al Nucleic Acids Res 2001 Jun 15;29(12):E56

Insert

floxed EGFP beta-gal

Use

Cre/Lox; Beta-gal

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 28, 2006

Availability

Academic Institutions and Nonprofits only
pTRANS_212

Plasmid

#91111

Purpose

Transformation, Type: T-DNA, Plant Selection: 2x35S:hpt II, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

35S

Available since

Sept. 29, 2017

Availability

Academic Institutions and Nonprofits only

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pJL006

Plasmid

#92329

Purpose

Express mEos3.2 tagged FtsZD212A mutant in E coli

Depositor

Jie Xiao

Article

Lyu et al Biopolymers. 2016 Oct;105(10):725-34. do

Insert

FtsZ

Use

Tags

mEos3.2

Expression

Bacterial

Mutation

D212A

Promoter

T5-lac

Available since

Aug. 9, 2017

Availability

Academic Institutions and Nonprofits only

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pX330_HR_Prnp_3

Plasmid

#78621

Purpose

pX330 vector encoding SpCas9 and a chimeric guide RNA targeting Prnp coding sequence

Depositor

Walker Jackson

Article

Kaczmarczyk et al PLoS One. 2016 Apr 29;11(4):e0154604. do

Insert

pX330_HR_Prnp_3

Use

CRISPR and Mouse Targeting

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

July 19, 2016

Availability

Academic Institutions and Nonprofits only

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AtFLS2pro::GUS

Plasmid

#87167

Purpose

expression pattern of AtFLS2

Depositor

Silke Robatzek

Article

Beck et al J Exp Bot. 2014 Dec;65(22):6487-98. doi:

Insert

AT5G46330 promoter

Use

Tags

GUS

Expression

Mutation

Promoter

AtFLS2

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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p183_LTJ_sgRNACD90.2_A

Plasmid

#82671

Purpose

sgRNA targeting murine CD90.2. Co-expresses SpCas9-2A-EGFP.

Depositor

Lukas Jeker

Article

Kornete et al J Immunol. 2018 Feb 14. pii: jimmunol.17

Insert

sgRNA targeting CD90.2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 17, 2018

Availability

Academic Institutions and Nonprofits only

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pJB073

Plasmid

#98912

Purpose

Express Dronpa tagged ZapB in E coli

Depositor

Jie Xiao

Article

Buss et al PLoS Genet. 2015 Apr 7;11(4):e1005128. d

Insert

ZapB

Use

Tags

Dronpa

Expression

Bacterial

Mutation

Promoter

T5-lac

Available since

Aug. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pTRANS_202

Plasmid

#91106

Purpose

Transformation, Type: T-DNA, Plant Selection: none, Viral Replication: ToLCV

Depositor

Daniel Voytas

Article

Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

No Promoter

Available since

July 14, 2017

Availability

Academic Institutions and Nonprofits only

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pHU6_Ch13_102010574-gRNA-for

Plasmid

#81218

Purpose

pHu6-gRNA-NT1_SpecR backbone, gRNA for targeting the 3' region of pCALNL_Ch13 reporter

Depositor

David Liu

Article

Chaikind et al Nucleic Acids Res. 2016 Aug 11. pii: gkw

Insert

hU6 expression of gRNA

Use

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

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pX330-sgRNA_Drosha_4

Plasmid

#79863

Purpose

CRISPR/Cas9 DROSHA

Depositor

Constance Ciaudo

Article

Cirera-Salinas et al J Cell Biol. 2017 Jan 18. pii: jcb.20160

Insert

sgRNA Drosha

Use

CRISPR and Mouse Targeting

Tags

Expression

Bacterial and Mammalian

Mutation

Promoter

Available since

Jan. 30, 2017

Availability

Academic Institutions and Nonprofits only