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Plasmid#140224PurposeConstitutive PGK driven expression of intein with miniVPR for 3-way split activatorDepositorInsertC-intein with miniVPR
UseTagsmCherryExpressionMammalianMutationPromoterPGKAvailable sinceApril 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-U6-Diras2_2-hSyn::mCherry.3xFLAG-WPRE
Plasmid#120394PurposepAAV plasmid expressing Diras2 shRNA2 under the U6 promoter and mCherry.3XFLAG under the hSyn promoterDepositorInsertDiras2 shRNA (Diras2 Mouse)
UseAAV and RNAiTagsmCherry.3XFLAGExpressionMutationPromoterU6Available sinceFeb. 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBMN mCherry-NBR1(F562A/Y731A/ΔUBD)
Plasmid#119692PurposeFor mammalian expression of LIR double mutant NBR1 without its UBDDepositorInsertNBR1 (NBR1 Human)
UseRetroviralTagsmCherryExpressionMammalianMutationF562A/Y731A & UBD deletion (1-906 ), See depo…PromoterAvailable sinceJan. 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pED55 pRS306 pRPS2-SteND-NLS-mCherry
Plasmid#68277PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7ND 2xNLS mCherry Non functional sensor with non-docking mutationDepositorInsertSte7ND NLS mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pED53 pRS306 pRPS2-Ste7DS NLS(4A)-mCherry
Plasmid#68275PurposeSKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4A) mCherry Non functional sensor with non-phosphorylatable NLSDepositorInsertSte7DS NLS(4A) mCherry
UseTagsmCherryExpressionYeastMutationPromoterpRPS2Available sinceSept. 14, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCru5-CCC/ACC-mEGFP-IRES-mCherry
Plasmid#49222PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWPT-CCC/ACC-mEGFP-IRES-mCherry
Plasmid#49231PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pWPT-GGG/ACC-mEGFP-IRES-mCherry
Plasmid#49230PurposeLentiviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseLentiviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterEF1-shortAvailable sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCru5-ACC/ACC-mEGFP-IRES-mCherry
Plasmid#49220PurposeRetroviral expression vector. Contains an altered Kozak sequence and/or upstream open reading frames to modulate expression at the level of translation.DepositorInsertsmEGFP
mCherry
UseRetroviral and Synthetic BiologyTagsSfuI site to create C-terminal fusionsExpressionMammalianMutationPromoterViral LTR (or CMV)Available sinceDec. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-Zim3-dCas9-P2A-mCherry
Plasmid#188779PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsZim3 KRAB-NLS fusionExpressionMutationPromoterSFFVAvailable sinceOct. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCBh_NLS_dCas13X_NLS-pA-U6-DR-BpiI-BpiI-DR-pSV40-EGFP-pA-pSV40-mCherry-pA
Plasmid#191796Purposevector for encoding a human codon-optimized dead Cas13X (dCas13X) driven by CBh promoter, guide RNAs compatible with Cas13X driven by hU6, EGFP driven by SV40 promoter, and mCherry driven by SV40 promoterDepositorInserthumanized dCas13X
UseCRISPRTagsExpressionMammalianMutationR84A, H89A, R739A, R740A, H745A, H746A, H747APromoterCBh, SV40, hU6Available sinceOct. 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-SFFV-dCas9-mCherry-ZIM3-KRAB
Plasmid#154473PurposeExpresses dCas9 fused to mCherry and the KRAB domain of ZIM3DepositorInsertZIM3 (ZIM3 Human)
UseLentiviralTagsHAExpressionMammalianMutationIncludes ZIM3 aa 1-100PromoterSFFVAvailable sinceOct. 5, 2020AvailabilityAcademic Institutions and Nonprofits only -
M31: pHR-SFFVp- TDP-43C::mCherry::SspB
Plasmid#122669PurposeC terminal domain of TDP43 (218-414) tagged with SspB and mCherry.DepositorInsertTDP43 (TARDBP Human)
UseLentiviralTagsmCherry-SspBExpressionMammalianMutationContains only amino acids 218-414PromoterSFFVAvailable sinceApril 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-IL35-IRES-mCherry-PGK-BFP
Plasmid#223210PurposeLentiviral vector - mCherry reporter and IL35 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertIL35 (IL12A Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHR-UCOE-EF1a-Zim3-dCas9-P2A-mCherry
Plasmid#188766PurposedCas9 with an N-term Zim3 KRAB-NLS fusion, a C-term HA-2xNLSDepositorInsertZim3-dCas9
UseLentiviralTagsHA-2xNLS and Zim3 KRAB-NLS fusionExpressionMutationPromoterEF1aAvailable sinceOct. 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX458-Dual-Guide-Donor-Cas9-H2B-mCherry
Plasmid#175570PurposeDonor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMutationXbaI site added upstream of U6 promotor. G to C …PromoterAvailable sinceOct. 7, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-PDL1-IRES-mCherry-PGK-BFP
Plasmid#223208PurposeLentiviral vector - mCherry reporter and PDL1 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertPDL1 (CD274 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-Ef1a-DIO hChR2(E123T/T159C)-mCherry
Plasmid#35510PurposeAAV expression of Ef1a-driven, cre-dependent, hChR2 variant for ultrafast optogenetic control.DepositorInserthChR2
UseAAVTagsmCherryExpressionMammalianMutationE123T and T159CPromoterEf1aAvailable sinceApril 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
CMV-dCas13X.1-REPAIRv2-SV40pA_CMV-mCherry-BGHpA_U6-BbsI-DR
Plasmid#171382PurposeExpression vector for encoding a human codon-optimized dCas13X.1-REPAIRv2 driven by CMV promoter,mCherry driven by CMV promoter and U6-driven crRNAs cloning site.DepositorInserthumanized dCas13X.1-REPAIRv2
UseTagsHAExpressionMammalianMutationPromoterCMV, U6Available sinceJuly 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR-GAL4-IL10-IRES-mCherry-PGK-BFP
Plasmid#223209PurposeLentiviral vector - mCherry reporter and IL10 for Gal4DBDVP64 synNotch receptors with a constitutive BFPDepositorInsertIL10 (IL10 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceDec. 16, 2024AvailabilityAcademic Institutions and Nonprofits only