Addgene

We narrowed to 6,532 results for: 11

Showing: 3781 - 3800 of 6532 results

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pcDNA3.1-MS2-HA-HsNot1iso1_1606-1840-shRNAres_AC

Plasmid

#148415

Purpose

Mammalian Expression of HsNot1iso1_1606-1840-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1_1606-1840-shRNAres (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsNot1iso1_1841-2376-shRNAres_AC

Plasmid

#148416

Purpose

Mammalian Expression of HsNot1iso1_1841-2376-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsNot1iso1_1841-2376-shRNAres (CNOT1 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-QHTRtoAAAA_AC

Plasmid

#148421

Purpose

Mammalian Expression of HsRck_296-472-QHTRtoAAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-QHTRtoAAAA (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-RKKtoAAA_AC

Plasmid

#148422

Purpose

Mammalian Expression of HsRck_296-472-RKKtoAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-RKKtoAAA (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-MS2-HA-HsRck_296-472-RQNtoAAA_AC

Plasmid

#148423

Purpose

Mammalian Expression of HsRck_296-472-RQNtoAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck_296-472-RQNtoAAA (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pCIneo-lambdaN-HA-HsRCK-RFQNRtoEDAAA_AC

Plasmid

#148429

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-E236Q-shRNAres_AC

Plasmid

#148450

Purpose

Mammalian Expression of HSRck-E236Q-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HSRck-E236Q-shRNAres (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-K135A-shRNAres_AC

Plasmid

#148451

Purpose

Mammalian Expression of HsRck-K135A-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-K135A-shRNAres (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-QHTRRKKtoAAAAAAA-shRNAres_AC

Plasmid

#148452

Purpose

Mammalian Expression of HsRck-QHTRRKKtoAAAAAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-QHTRRKKtoAAAAAAA-shRNAres (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
pT7-EGFP-C1-HsRCK-QHTRtoAAAA-shRNAres_AC

Plasmid

#148453

Purpose

Mammalian Expression of HsRck-QHTRtoAAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-QHTRtoAAAA-shRNAres (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HsRCK-RFQNRtoEDAAA-shRNAres_AC

Plasmid

#148454

Purpose

Mammalian Expression of HsRck-RFQNRtoEDAAA-shRNAres

Depositor

Elisa Izaurralde

Article

Kuzuoglu-Ozturk et al EMBO J. 2016 Jun 1;35(11):1186-203. doi:

Insert

HsRck-RFQNRtoEDAAA-shRNAres (DDX6 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Availability

Academic Institutions and Nonprofits only

Enlarge
Sc++

Plasmid

#155011

Purpose

Expresses the codon-optimized, engineered Sc++ Cas9 nuclease in mammalian cells

Depositor

Joseph Jacobson

Article

Chatterjee et al Nat Biotechnol. 2020 May 11. pii: 10.103

Insert

Sc++

Use

Tags

Expression

Mammalian

Mutation

T1227K, Loop Substitution from S. anginosus

Promoter

EF1ɑ

Availability

Academic Institutions and Nonprofits only

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pCR3-vsv-dCEN-Incenp

Plasmid

#108479

Purpose

expression of vsv-dCEN-INCENP

Depositor

Susanne Lens

Article

Vader et al Mol Biol Cell. 2007 Nov;18(11):4553-64.

Insert

dCEN-INCENP (INCENP Human)

Use

Tags

VSV

Expression

Mammalian

Mutation

CEN box removed

Promoter

CMV

Availability

Academic Institutions and Nonprofits only

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MSCV-HA-Nr4a1

Plasmid

#160941

Purpose

Generation of retrovirus for the overexpression of Nr4a1

Depositor

Matthew Steinhauser

Article

Zhang et al J Clin Invest. 2018 Nov 1;128(11):4898-4

Insert

Nr4a1 (Nr4a1 Mouse)

Use

Retroviral

Tags

HA tag

Expression

Mammalian

Mutation

Promoter

MSCV-LTR

Availability

Academic Institutions and Nonprofits only

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pAS_4xBstTyrT(CUA)_EcoTyrRS-FLAG

Plasmid

#140018

Purpose

Plasmid with 4xBstTyrT(CUA) cassette and E. coli TyrRS for amber suppression; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

EcoTyrRS

Use

Tags

FLAG

Expression

Mammalian

Mutation

Y37L, D182S, F183M, L186A

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-AcKRS

Plasmid

#140021

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of acetyl-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

AcKRS-3 (pylS Methanosarcina mazei)

Use

Tags

FLAG

Expression

Mammalian

Mutation

L301M, L305I, Y306F, L309A, C348F

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pAS_4xMma PylT_FLAG-PcKRS

Plasmid

#140022

Purpose

Plasmid with 4xMmaPylT cassette and MmaPylRS for amber suppression and incorporation of photocaged-lysine; for transient or stable piggyBac-mediated integration

Depositor

Simon Elsaesser

Article

Meineke et al Cell Rep Methods. 2023 Nov 20;3(11):1006

Insert

PcKRS (pylS Methanosarcina bakeri)

Use

Tags

FLAG

Expression

Mammalian

Mutation

M241F, A267S, Y271C, L274M

Promoter

EF1

Availability

Academic Institutions and Nonprofits only

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pCRI004-pYFAC-riboB-PgpdA-dLbCas12a-VPR-TtrpC

Plasmid

#140197

Purpose

Episomal expression of dLbCas12a-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dLbCas12a-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Expression

Mutation

D832A DNase deactivated

Promoter

PgpdA

Availability

Academic Institutions and Nonprofits only

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pCRI006-pYFAC-riboB-PgpdA-dSpCas9-VPR-TtrpC

Plasmid

#140199

Purpose

Episomal expression of dSpCas9-VPR. Filamentous fungi vector with AMA1 and riboB selection marker.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Insert

dSpCas9-VPR

Use

CRISPR and Synthetic Biology; Expression in asper…

Tags

VPR (VP64-p65-Rta), NLS

Expression

Mutation

Promoter

PgpdA

Availability

Academic Institutions and Nonprofits only

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pCRI018-pKW20088-PelcA-mCherry-TtrpC-PU3-E1-Cas9Scaffold-8xT

Plasmid

#140205

Purpose

Proof-of-concept of CRISPR/dSpCas9-VPR activation, encoding PelcA-mCherry along with the sgRNA E1 expression cassette, targeting PelcA.

Depositor

Yit Heng Chooi

Article

Roux et al ACS Synth Biol. 2020 Jun 11. doi: 10.102

Inserts

mCherry
sgRNA E1

Use

CRISPR and Synthetic Biology; Proof-of-concept te…

Tags

Expression

Mutation

G174D

Promoter

PelcA and U3

Availability

Academic Institutions and Nonprofits only

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