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Plasmid#171604PurposeExpresses the SNAP-tagged MIDAS domain of S. pombe Mdn1 (a.a. 4381-4717) in bacteria.DepositorInsertMIDAS domain of S. pombe Mdn1 (a.a. 4381-4717)
UseTagsHis6 tag and SNAP tagExpressionBacterialMutationPromoterAvailable sinceJuly 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tsc2-g1)-PGKpuroBFP-W
Plasmid#105039PurposeLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFPDepositorInsertTsc2 (Tsc2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Tsc2-g2)-PGKpuroBFP-W
Plasmid#105040PurposeLentiviral gRNA plasmid targeting mouse Tsc2 , co-expression of TagBFPDepositorInsertTsc2 (Tsc2 Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
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UKNP.1.12.1
Plasmid#97384PurposeExpression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)DepositorInsertHCV E1E2
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCINeoFlag ALIX; I212D
Plasmid#89861PurposeExpresses full length Flag-tagged ALIX with mutation I212D in mammalian cells; internal ID WISP05-117DepositorInsertALIX (PDCD6IP Human)
UseTagsFlagExpressionMammalianMutationMutated isoleucine 212 to aspartic acidPromoterCMVAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
VAMP3(Δ71)-mCherry
Plasmid#92425PurposeExpression of fusion incompetent VAMP3 (del71) C-terminally conjugated to mCherry.DepositorInsertVAMP3 (Vamp3 Mouse)
UseTagsmCherryExpressionMammalianMutationdeleted leucine 71PromoterCMVAvailable sinceJuly 12, 2017AvailabilityAcademic Institutions and Nonprofits only -
pJK-caCas9-NatMX-Neut5L-Leu2 drive
Plasmid#89578PurposecaCas9 integrating vector into the Neut5L locus with gene drive for targeting Leu2 locusDepositorInsertLeu2 gene drive
UseTagsExpressionYeastMutationPromoterAvailable sinceOct. 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
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DsRed +9e
Plasmid#191766PurposeExpression of DsRed fused to a positively charged polypeptide (2 x KSG) . Overall charge on tetramer: +9eDepositorInsertDsRed
UseTagsN terminal fusion of positively charged polypetod…ExpressionBacterial and MammalianMutationPromoterCMVAvailable sinceFeb. 2, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Nelfcd-g1)-PGKpuroBFP-W
Plasmid#105025PurposeLentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFPDepositorInsertNelfcd (Th1l Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pKLV2-U6gRNA5(Nelfcd-g2)-PGKpuroBFP-W
Plasmid#105026PurposeLentiviral gRNA plasmid targeting mouse Nelfcd , co-expression of TagBFPDepositorInsertNelfcd (Th1l Mouse)
UseCRISPR and LentiviralTagsExpressionMammalianMutationPromoterU6Available sinceSept. 4, 2019AvailabilityAcademic Institutions and Nonprofits only -
pLL7.0-iRFP670-micro
Plasmid#135954PurposeExpresses an iLID micro (SspB R73Q)-iRFP670 fusion in mammalian cells.DepositorInsertiLID micro (SspB R73Q)
UseLentiviralTagsiRFP670ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 11, 2020AvailabilityAcademic Institutions and Nonprofits only -
LN-entry
Plasmid#128012PurposeVector containing the lambdaN-entry expression cassette and a separate expression cassette driving mCherry via the traffic jam enhancerDepositorInsertLN-3xFlag
UseLamdan entry vectorTagsLN-3xFlagExpressionMutationPromoterAvailable sinceAug. 2, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:mTREK-1(K271Q)
Plasmid#133270PurposeX. laevis expression vector. It will generate the mouse TREK-1 channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1) (Kcnk2 )
UseOocyte expressionTagsExpressionMutationK271QPromoterAvailable sinceApril 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pGEMHE:hTRAAK(Q258K)
Plasmid#133077PurposeX. laevis expression vector. It will generate the human TRAAK channelDepositorInsertPotassium channel subfamily K member 2 (KCNK2, TREK-1), Q258K (Kcnk2 Mouse)
UseTagsExpressionMutationQ258KPromoterT7Available sinceOct. 30, 2021AvailabilityAcademic Institutions and Nonprofits only -
ET1-human LRRC33
Plasmid#111603PurposeExpress human LRRC33 in 293T cellsDepositorInserthuman LRRC33 (NRROS Human)
UseTagsPreScission/TEV/ProteinC/6xHIS/SBP/ExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFOSGE_DUM1H01
Plasmid#71090PurposeGateway entry cloneDepositorInsertnej (nej Fly)
UseGateway entry vectorTagsExpressionMutationPromoterAvailable sinceFeb. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
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33-G-X1-pEF1-puro
Plasmid#111604PurposeExpress human 33-G-X1 in 293T cellsDepositorInserthuman LRRC33 and GARP chimeria (NRROS Human)
UseTagsFlag tagExpressionMammalianMutationPromoterEF1aAvailable sinceJuly 25, 2018AvailabilityAcademic Institutions and Nonprofits only