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Plasmid#134137PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, R155APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW082
Plasmid#134136PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, R236APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW088
Plasmid#134135PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, K223A K226APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW085
Plasmid#134134PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, K183A K184APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSG5 N-RFP SADBs
Plasmid#67153PurposepSG5 containing N-terminally RFP tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsRFPExpressionMammalianMutationPromoterSV40Available sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSG5 N-HA SADBs
Plasmid#67149PurposepSG5 containing N terminally HA tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsHAExpressionMammalianMutationPromoterSV40, T7Available sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCNL-N1_TUBB5
Plasmid#65679PurposeExpresses beta-tubulin-CNL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsCNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 17, 2015AvailabilityAcademic Institutions and Nonprofits only -
pGEX-αTAT1[2-236]
Plasmid#27101DepositorInsertαTubulin K40 acetyltransferase (ATAT1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
LSS-SGFP2-aTub
Plasmid#112936PurposeVisualization of microtubulesDepositorInsertalpha tubulin
UseTagsLSS-SGFP2ExpressionMammalianMutationPromoterCMVAvailable sinceAug. 30, 2018AvailabilityAcademic Institutions and Nonprofits only -
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pET21 E2F1
Plasmid#90313PurposeExpresses human E2F1 in bacteriaDepositorInsertE2F1 (E2F1 Human)
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceAug. 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pEF5B-FRT-GFP-αTAT1[D157N]
Plasmid#27100DepositorInsertαTubulin K40 acetyltransferase (ATAT1 Human)
UseTagsGFP and S-tagExpressionMammalianMutationD157NPromoterAvailable sinceJan. 5, 2011AvailabilityAcademic Institutions and Nonprofits only -
pDL023_Tub-rb_IgG_HC
Plasmid#193620Purposeα-Tubulin heavy chain variable region + rabbit heavy chain constant regions + exogenous N-term signal peptide. For co-expression with pDL024 in HEK293 cells.DepositorInsertAnti-Tubulin scFv heavy variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pX459-HypaCas9-TUBA1B_sgRNA
Plasmid#183889PurposepX459V2.0-HypaCas9 plasmid with TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.DepositorInsertTUBA1B sgRNA spacer (TUBA1B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pX459-HypaCas9-mR2-TUBA1B_sgRNA
Plasmid#183888PurposepX459V2.0-HypaCas9 plasmid with mRuby2 reporter and TUBA1B sgRNA for N-terminal tagging of alpha-tubulin in human cells.DepositorInsertTUBA1B sgRNA spacer (TUBA1B Human)
UseCRISPRTagsExpressionMammalianMutationPromoterU6Available sinceMay 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDL024_Tub-rb_IgG_LC
Plasmid#193621Purposeα-Tubulin light chain variable region + rabbit light chain constant region + exogenous N-term signal peptide. For co-expression with pDL023 in HEK293 cells.DepositorInsertAnti-Tubulin scFv light variable + rabbit constant chain
UseTagssignal peptideExpressionMammalianMutationPromoterCMVAvailable sinceDec. 21, 2022AvailabilityIndustry, Academic Institutions, and Nonprofits -
pBABR IRFP-Bcd-LEXY
Plasmid#182595PurposeDrosophila integration plasmid expressing IRFP-Bcd-LEXY fusion protein under the maternal tubulin promoter.DepositorInsertBcd (bcd Fly)
UseTagsIRFP and LEXYExpressionInsectMutationPromotermaternal tubulinAvailable sinceAug. 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS/U6/RNAi SadBs shRNA mouse
Plasmid#67152PurposepBS/U6 containing shRNA to mouse SADBsDepositorInsertBRSK1 (Brsk1 Mouse)
UseTagsExpressionMammalianMutationPromoterU6Available sinceNov. 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
pJS278
Plasmid#134139PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD)DepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationaa 132-255, R155APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pEGFP C1 SADBs
Plasmid#67154PurposepEGFP containing N-terminally EGFP Tagged mouse SADBsDepositorInsertBrsk1 (Brsk1 Mouse)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceJuly 8, 2015AvailabilityAcademic Institutions and Nonprofits only