-
Plasmid#201765PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->HA-mtLaG2
Plasmid#162225PurposeLentiviral vector for the expression of HA GFP nanobody Lag2 in mammalian cellsDepositorInsertanti-GFP nanobody LaG2
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
bactin:GFP-LAP2b
Plasmid#105980Purposetransgenesis, ubiquitous expressionDepositorInsertLAP2b
UseTagsGFPExpressionBacterialMutationPromoterAvailable sinceMarch 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP
Plasmid#162233PurposeLentiviral vector for the expression of GFP nanobody LaG16LaG2 -IFP : TRE3GS-->GssmycLaG16LaG2_P2A_NLSIFP in mammalian cellsDepositorInsertanti-GFP nanobody lag16lag2 and IFP
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pABY-T1
Plasmid#201764PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-P-RAN-GFP1
Plasmid#106408PurposeP-RAN-GFP1 (RANbody GFP1::vHRP)DepositorInsertP-RAN-GFP1
UseTagsHA and HisExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_BAP_his
Plasmid#210402PurposeFor expression of VHH with C-terminal biotin acceptor peptide and his tagDepositorTypeEmpty backboneUseTagsbiotin acceptor peptide-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_cys_his
Plasmid#210403PurposeFor expression of VHH with C-terminal cysteine and his tagDepositorTypeEmpty backboneUseTagscys-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his_flag
Plasmid#210404PurposeFor expression of VHH with C-terminal his and Flag tagsDepositorTypeEmpty backboneUseTagshis6-flagExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his
Plasmid#210405PurposeFor expression of VHH with C-terminal his tagDepositorTypeEmpty backboneUseTagshis6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_sortase_his
Plasmid#210406PurposeFor expression of VHH with C-terminal sortase and his tagsDepositorTypeEmpty backboneUseTagssortase-his6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_snap_his
Plasmid#210407PurposeFor expression of VHH with C-terminal snap protein and his tagDepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pOPINVHH_his_myc
Plasmid#210526PurposeFor expression of VHH with C-terminal his and myc tagsDepositorTypeEmpty backboneUseTagsHis6-mycExpressionBacterialMutationPromoterlacAvailable sinceMarch 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2
Plasmid#162454PurposeYeast display plasmid intended for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA) and expression of N-terminal fusions with Aga2p yeast agglutinin.DepositorTypeEmpty backboneUseShuttle vector bacteria/yeastTagsDnbALFA nanobody and Ha and mycExpressionMutationPromoterAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHR_EF1a-->myc-mtLaG2
Plasmid#162224PurposeLentiviral vector for constitutive expression of Myc GFP nanobody Lag2 in mammalian cellsDepositorInsertanti-GFP nanobody LaG2
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pABY-T3
Plasmid#201766PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pABY-T4
Plasmid#201767PurposeFor C-terminal tagging of a protein-of-interest with the ALFA tag using S2/S3 primers in yeastDepositorInsertALFA tag
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCMV-P-RAN-Gelsolin
Plasmid#106413PurposeP-RAN-Gelsolin (RANbody Gelsolin::vHRP)DepositorInsertP-RAN-Gelsolin
UseTagsHA and HisExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCMV-P-RAN-GFP41
Plasmid#106407PurposeP-RAN-GFP41 (RANbody GFP41::vHRP)DepositorInsertP-RAN-GFP41
UseTagsHA and HisExpressionMammalianMutationPromoterAvailable sinceFeb. 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJYDN2p
Plasmid#162455PurposePositive control plasmid for co-cultivation labeling by using designed ALFA-tag binding nanobody (DnbALFA).DepositorInsertAga2p-DnbALFA (AGA2 )
UseShuttle vector bacteria/yeastTagsDnbALFA nanobody and HA and mycExpressionMutationPromoterGAL1Available sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only