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Plasmid#191533PurposePrig-3-LoxP::EBFP::LoxP::GtACR2::wCherry unc-54 3' UTR C.elegans AVA neuron expression of GtACR2 RFPDepositorInsertGtACR2
UseTagswCherryExpressionWormMutationPromoterPrig-3Available sinceNov. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
MTK1_015
Plasmid#123678PurposeEncodes ConLS, chicken hypersensitivity site 4 insulator and locus of X-over P1 (loxP) as a Type 1 part to be used in the MTK systemDepositorInsertConnector LS-2xHS4-LoxP
UseTagsExpressionMammalianMutationPromoterAvailable sinceDec. 13, 2019AvailabilityAcademic Institutions and Nonprofits only -
pK068.CAG-FRT-stop-FRT-EGFP-ires-tTA-WPRE (Supernova)
Plasmid#85008PurposeFor Flpe/FRT-based Supernova-GFP, pK068 should be used with pK036. The GFP fragment of pK068 can be replaced with another gene by SalI and EcoRV.DepositorInsertEGFP-ires-tTA
UseTagsExpressionMammalianMutationPromoterAvailable sinceNov. 30, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-CreLite
Plasmid#131785PurposeAAV donor/transfer vector expressing CreLite system components, PhyBΔCreC and PIF6CreN, driven by CBh promoterDepositorInsertPhyBCreC-P2A-PIF6CreN
UseAAV, Cre/Lox, and Synthetic BiologyTagsExpressionMammalianMutationPromoterCBhAvailable sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pMADM-alpha
Plasmid#36890DepositorInsertsGFP
tdTomato-3Myc
beta-globin intron
Neo
ATG (T)
GFP
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, deleted…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 17, 2012AvailabilityAcademic Institutions and Nonprofits only -
pTol2-PcyA-IRES-HO1
Plasmid#131787PurposeTol2 bicistronic vector expressing PcyA and HO1 for Phycocyanobilin (PCB) synthesis, driven by heatshock promoter hsp70.DepositorInsertPcyA and HO1
UseSynthetic Biology; Zebrafish tol2 transposonTagsMTS (Mitochondrial Targeting Signal from subunit …ExpressionMammalianMutationPromoterhsp70Available sinceJan. 9, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJH4316
Plasmid#200171PurposePflp-18 LoxP EBFP (stop) LoxP twk-40(gf) GFP unc-54 3' UTR C.elegans AVA and other neurons expression of twk-40(gf) GFPDepositorInserttwk-40 (twk-40 Nematode)
UseTagsGFPExpressionWormMutationPromoterPflp-18Available sinceMay 7, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCALNL-tdTomato
Plasmid#194205PurposeCre/LoxP-dependent expression of a tdTomato. Expression of Cre excises a STOP codon before tdTomato, facilitating fluorescence expression of tdTomato.DepositorInserttdTomato
UseCre/LoxTagsExpressionMammalianMutationWTPromoterAvailable sinceJan. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
pT2K-LRL-IRES-Luc2
Plasmid#109230PurposeFor Tol2 transposon-mediated stable expression of LRL and IRES-luciferaseDepositorInsertLRL and Luc2
UseTagsExpressionMammalianMutationPromoterCAGGSAvailable sinceMay 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pBT272_(pRosa26-GTET)
Plasmid#36882DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
diphteria toxin A
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
TV-hPITX3-Reverse
Plasmid#22208DepositorUseZfn donorTagsExpressionMutationPromoterAvailable sinceOct. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pBT270_(pCA-G-intron(Neo)-tTA2-iiTRE-tdT3Mycii)
Plasmid#36881DepositorInsertsGFP
tTA2
beta-globin intron
Neo
tdT-3Myc
insulator
UseTags3 Myc tagsExpressionMammalianMutationdeleted nucleotides after nucleotide 274, inserti…PromoterCAG (chicken beta actin promoter and CMV enhancer…Available sinceAug. 30, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_LEU2_II
Plasmid#109061Purposeyeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites downstream of LEU2. For teaching purposes.DepositorInsertLEU2 (LEU2 Budding Yeast, Zygosaccharomyces rouxii)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterLEU2Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pT3-EF1aH-PIK3CA H1047R
Plasmid#180031PurposeThis plasmid is in the pT3-EF1a vector without loxP sites flanking the inverted repeats of SB (sleeping beauty) sequence. Therefore this plasmid can be used in LoxP mice.DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseTagsHA tagExpressionMammalianMutationH1047RPromoterEF1aAvailable sinceMarch 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBCSK_LEU2_I
Plasmid#109060Purposeyeast shuttle vector for insertion into yeast chromosomes in pBCSK backbone. LoxP sites upstream of LEU2. For teaching purposesDepositorInsertLEU2 (LEU2 Budding Yeast, Zygosaccharomyces rouxii)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterLEU2Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCambia-loxKan
Plasmid#199590Purposebasic plant vector with a plant-selectable kanamycin resistance cassette flanked by both loxP and FRT sites for sustainable plant engineering with subsequent marker removal possibilityDepositorInsertFRT-lox66-NosP-KanR-NosT-lox71-FRT
UseCre/LoxTagsExpressionPlantMutationPromoterNosAvailable sinceJuly 5, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
1782_pAAV-U6-Ai9-Sa-gRNA2-CB-SACas9-HA-OLLAS-spA_C
Plasmid#135978PurposegRNA against the right loxP site of the Ai9 alleleDepositorInsertAi9 gRNA2
UseAAV, CRISPR, and Mouse TargetingTagsHAExpressionMutationPromoterCBAvailable sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
1781_pAAV-U6-Ai9-Sa-gRNA1-CB-SACas9-HA-OLLAS-spA_C
Plasmid#135979PurposegRNA against the left loxP site of the Ai9 alleleDepositorInsertAi9 gRNA1
UseAAV, CRISPR, and Mouse TargetingTagsHAExpressionMutationPromoterU6Available sinceJan. 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSA53
Plasmid#35115DepositorInsertcallys5 flanked by loxp
UseYeast replicatingTagsExpressionMutationPromoterAvailable sinceAug. 29, 2012AvailabilityAcademic Institutions and Nonprofits only -
pBSIIKS_HIS3_I
Plasmid#109062Purposeyeast shuttle vector with deletable CEN6-ARSH4 cassette in pBIIKS backbone. LoxP site upstream of HIS3. For teaching purposes.DepositorInsertHIS3 (HIS3 Budding Yeast, Zygosaccharomyces rouxii)
UseCre/LoxTagsExpressionBacterial and YeastMutationPromoterHIS3Available sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only