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Plasmid#122589PurposeExpression of human inositol 1,4,5-trisphosphate 3-kinase BDepositorInsertinositol 1,4,5-trisphosphate 3-kinase B (ITPKB Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 10, 2019AvailabilityAcademic Institutions and Nonprofits only -
AcpP pet29a C-terminal His Tag
Plasmid#75016PurposeExpresses the acyl carrier protein (ACP) from the E. coli fatty acid synthaseDepositorInsertAcpP (acpP E. coli)
UseTagsHis TagExpressionBacterialMutationPromoterT7Available sinceMay 18, 2016AvailabilityAcademic Institutions and Nonprofits only -
CMV Marina-T2A-nls-mCherry
Plasmid#74216Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertfluorescent protein voltage sensor
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterCMVAvailable sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Golden-Gate-Esp3I-Destination
Plasmid#51278PurposeCAG promoter-driven expression vector suitable for ligation of multiple inserts by Golden Gate Cloning using Esp3IDepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterCAGAvailable sinceMarch 4, 2014AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam mse LaminA
Plasmid#182674PurposeLentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.DepositorInsertLamin A (Lmna Mouse)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianMutationPromoterHSPAvailable sinceApril 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
pLenti GW V5 Eco/Dam hum LaminA
Plasmid#182673PurposeLentiviral eukaryotic expression vector with E.Coli Dam methylation fused to human Lamin A. Used in DamID experiments to methylate DNA that interacts in the proximity of Lamin A.DepositorInsertLamin A (LMNA Human)
UseLentiviralTagsE. Coli Dam and V5ExpressionMammalianMutationPromoterHSPAvailable sinceMarch 22, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2D)
Plasmid#29774DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pPW3760 : CMVd3-HsIRE1-K599A_KinaseDead-HaloTag
Plasmid#185682PurposeLow-level transient expression of kinase-dead K599A HsIRE1 with a C-terminal HaloTag.DepositorInsertERN1 (ERN1 Synthetic, Human)
UseTagsHaloTagExpressionMammalianMutationK599APromoterAvailable sinceMarch 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
K84M kinase-dead Nuak1-3xflag
Plasmid#97219PurposePlasmid expressing kinase-dead mutant Nuak1DepositorInsertNuak1 (NUAK1 Human)
UseTagsFLAGExpressionBacterial and MammalianMutationKinase-dead K84MPromoterT7Available sinceAug. 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
hSyn Marina-T2A-nls-mCherry
Plasmid#85843Purposegenetically-encoded fluorescent voltage indicatorDepositorInsertGEVI Marina
UseTagsmCherryExpressionMammalianMutationCi-VSP contains R217Q mutation; super ecliptic pH…PromoterhSyn1Available sinceJan. 30, 2017AvailabilityAcademic Institutions and Nonprofits only -
inactive cdk1 FRET sensor (2328)
Plasmid#26065DepositorInsertCdk1 FRET Sensor- Inactive
UseTagsCFP variant, FLAG, and YFP variantExpressionMammalianMutationSer 126 mutated to AlaPromoterAvailable sinceSept. 15, 2010AvailabilityAcademic Institutions and Nonprofits only -
pET empty polycistronic destination vector (2Z)
Plasmid#29776DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pMD19-Cas9_handle-S.pyogenes_terminator-PBBa_J23117-Cmr
Plasmid#190794PurposeTemplate vector to amplify pieces for creating multiplex sgRNA-arrays.DepositorInsertsgRNA-scaffold only
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterBBa_J23117Available sinceNov. 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaPH
Plasmid#22930PurposeMammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PH domainDepositorInsertPHLPP1-deltaPH (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationIncludes 127-1205 aa. Deleted amino-terminal PH d…PromoterCMVAvailable sinceFeb. 3, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-HA-PHLPP1(N-terminal truncation)-deltaC
Plasmid#22931PurposeMammalian expression of a HA tagged, N-terminal truncation of human PHLPP1 containing a deletion of the PDZ binding motif (delta C)DepositorInsertPHLPP1-deltaC (PHLPP1 Human)
UseTagsHAExpressionMammalianMutationPHLPP-deltaC, deletion of PDZ binding domain--the…PromoterCMVAvailable sinceFeb. 4, 2010AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-NES-DEVD-mCardinal-NLS
Plasmid#164052PurposeFluorescent reporter for caspase-3DepositorInsertNES-mCardinal-NLS
UseTagskozak sequenceExpressionMammalianMutationPromoterCMV promotorAvailable sinceFeb. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS416-PHO5-GFP-Opi1 + terminator
Plasmid#58836PurposeExpresses GFP-Tagged Opi1 + Terminator in yeastDepositorInsertOpi1p (OPI1 Budding Yeast)
UseTagsGFPExpressionYeastMutationPromoterPHO5Available sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
413-Met25 Kar2 TorsinA GFP
Plasmid#32119DepositorInsertTorsinA (TOR1A Human)
UseTagsGFP and Kar2ExpressionYeastMutationPromoterAvailable sinceSept. 30, 2011AvailabilityAcademic Institutions and Nonprofits only -
pGEX6P1-human LIC1 C-terminal half
Plasmid#74599Purposeused for expression in E. coli and purification. N-terminal GST tag and C-terminal Strep tag, aa 389-523DepositorInserthuman dynein light intermediate chain 1 amino acids 389-523 (DYNC1LI1 Human)
UseTagsN-terminal GST and Strep IIExpressionBacterialMutationamino acids 389-523 of LIC1PromoterT7Available sinceMay 2, 2016AvailabilityAcademic Institutions and Nonprofits only -
pPuro3.1(+)_Strep-Lrrk2(delKinase)-Myc
Plasmid#161586Purposeexpression of Myc-tagged mouse Lrrk2 without kinase domainDepositorInsertLrrk2 (Lrrk2 Mouse)
UseTagsMyc tag and StrepIIExpressionMammalianMutationdeletion: AA1871-2540PromoterCMVAvailable sinceDec. 1, 2020AvailabilityAcademic Institutions and Nonprofits only