Addgene

We narrowed to 59,462 results for: cloning

Showing: 361 - 380 of 59462 results

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CJW4617

Bacterial Strain

#177118

Purpose

E. coli strain carrying IPTG-inducible gfp-muNS gene for monitoring the mobility of cytoplasmic particles.

Depositor

Christine Jacobs-Wagner

Article

Parry et al Cell. 2014 Jan 16;156(1-2):183-94. doi:

Bacterial Resistance

Kanamycin

Available since

Jan. 6, 2022

Availability

Academic Institutions and Nonprofits only
pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

Plasmid

#48295

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

His6

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)

Plasmid

#48291

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Sumo

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)

Plasmid

#48292

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Thioredoxin

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C)

Plasmid

#48286

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and MBP

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pET His6 Mocr TEV cloning vector with BioBrick polycistronic restriction sites (9O)

Plasmid

#48289

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and Mocr

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 24, 2013

Availability

Academic Institutions and Nonprofits only

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pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

Plasmid

#187320

Purpose

dCas9 expression vector with cloning site for C terminal fusions

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al bioRxiv. (PrePrint)

Type

Empty backbone

Use

CRISPR and Lentiviral

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

Available since

May 6, 2024

Availability

Academic Institutions and Nonprofits only

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pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)

Plasmid

#48287

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6 and msfGFP

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

Plasmid

#48285

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6

Expression

Bacterial

Mutation

Promoter

Available since

Nov. 5, 2013

Availability

Academic Institutions and Nonprofits only

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CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

Plasmid

#53959

Purpose

allows Cre-mediated expression of transgene

Depositor

Jeffrey Robbins

Article

Nakamura et al Proc Natl Acad Sci U S A. 2009 Sep 8;106

Type

Empty backbone

Use

Cre/Lox

Tags

Lox-CAT-Lox

Expression

Mammalian

Mutation

Promoter

CMV enhancer, chicken actin promoter

Available since

June 30, 2014

Availability

Academic Institutions and Nonprofits only
pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

Plasmid

#48296

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

His6 and MBP

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

Plasmid

#161926

Purpose

Lentiviral backbone for GoldenGate cloning effectors as a fusion with rTetR(SE-G72P)-3XFLAG

Depositor

Michael Bassik, Lacra Bintu

Article

Tycko et al Cell. 2020 Dec 9. pii: S0092-8674(20)315

Insert

rTetR(SE-G72P)

Use

Lentiviral and Synthetic Biology

Tags

3XFLAG

Expression

Mammalian

Mutation

Promoter

pEF

Available since

Feb. 10, 2021

Availability

Academic Institutions and Nonprofits only

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rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

Plasmid

#122450

Purpose

Expression of inositol 1,4,5-trisphosphate 3-kinase A

Depositor

Christophe Erneux

Article

Takazawa et al Biochem J. 1990 Nov 15;272(1):107-12.

Insert

inositol 1,4,5-trisphosphate 3-kinase A (Itpka Rat)

Use

Tags

beta-galactosidase

Expression

Bacterial

Mutation

coding sequence from V66 till stop codon

Promoter

Available since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

Plasmid

#48297

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

StrepII and msfGFP

Expression

Insect

Mutation

Promoter

Available since

Sept. 23, 2013

Availability

Academic Institutions and Nonprofits only

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pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

Plasmid

#55217

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Available since

July 9, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

Plasmid

#55216

Depositor

Scott Gradia

Article

Gradia et al Methods Enzymol. 2017;592:1-26. doi: 10.

Type

Empty backbone

Use

Tags

SNAPf and TwinStrepII

Expression

Insect

Mutation

Promoter

Available since

June 16, 2014

Availability

Academic Institutions and Nonprofits only

Enlarge
pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

Plasmid

#198054

Purpose

All-in-one lentiviral backbone for doxycycline-inducible expression of a 3xFLAG-tagged protein of interest, which can be inserted with Golden Gate cloning

Depositor

Lacra Bintu

Article

Ludwig et al Cell Syst. 2023 Jun 21;14(6):482-500.e8.

Inserts

rTetR(SE-G72P)-VP48-T2A-mCherry-BSD
3xFLAG-insert

Use

Lentiviral and Synthetic Biology

Tags

3xFLAG

Expression

Mammalian

Mutation

Promoter

TRE3G and pEF

Available since

June 27, 2023

Availability

Academic Institutions and Nonprofits only

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UQ4884

Bacterial Strain

#37161

Depositor

Gary Roberts

Article

Kerby et al J Bacteriol. 2008 May;190(9):3336-43. Ep

Bacterial Resistance

Ampicillin

Species

Burkholderia xenovorans

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
UQ4802

Bacterial Strain

#37160

Depositor

Gary Roberts

Article

Kerby et al J Bacteriol. 2008 May;190(9):3336-43. Ep

Bacterial Resistance

Ampicillin

Species

Burkholderia xenovorans

Available since

June 21, 2012

Availability

Academic Institutions and Nonprofits only
Corynebacterium glutamicum::RFP

Bacterial Strain

#79642

Purpose

Expresses RFP from the chromosome of Corynebacterium glutamicum ATCC 13032 (ptac promoter unrepressed; no LacI present)

Depositor

Timothy Lu

Article

Cleto et al ACS Synth Biol. 2016 Feb 16.

Bacterial Resistance

Gentamicin

Available since

Nov. 4, 2016

Availability

Academic Institutions and Nonprofits only

Showing: 361 - 380 of 59462 results

Results per page:

Sort results by:

CJW4617

pFastBac His6 TEV cloning vector with BioBrick polycistronic restriction sites (11B)

pET His6 Sumo TEV cloning vector with BioBrick polycistronic restriction sites (9S)

pET His6 Thioredoxin TEV cloning vector with BioBrick polycistronic restriction sites (9T)

pET His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (9C)

pET His6 Mocr TEV cloning vector with BioBrick polycistronic restriction sites (9O)

pJT216 Lenti pEF-dCas9-3XFLAG-LibCloneSite-T2A-tagBFP-P2A-Blast

pET His6 msfGFP TEV cloning vector with BioBrick polycistronic restriction sites (9GFP)

pET C-terminal TEV His6 cloning vector with BioBrick polycistronic restriction sites (9Bc)

CAG-lox-CAT-lox-unique Bam cloning site-hGH polyA (Clone 17)

pFastBac His6 MBP TEV cloning vector with BioBrick polycistronic restriction sites (11C)

pJT126 lenti pEF-rTetR(SE-G72P)-3XFLAG-LibCloneSite-T2A-mCherry-BSD-WPRE

rat brain inositol 1,4,5-trisphosphate 3-kinase A (Clone C5, partial sequence from V66)

pFastBac StrepII msfGFP TEV cloning vector with BioBrick polycistronic restriction sites(11R-GFP)

pFastBac TEV SNAPf Prescission TwinStrepII cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V3)

pFastBac TwinStrepII Prescission SNAPf TEV cloning vector with BioBrick polycistronic restriction sites (11-SNAP-V1)

pCL040 lenti TRE-3xFLAG-LibCloneSite pEF-rTetR(SE-G72P)-VP48-T2A-mCherry-BSD-WPRE

UQ4884

UQ4802

Corynebacterium glutamicum::RFP

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