Addgene

We narrowed to 613 results for: ca2

Showing: 361 - 380 of 613 results

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pRSM42

Plasmid

#113942

Purpose

Constitutive bacterial expression of red fluorescent Ca2+ sensor jRGECO1a

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

jRGECO1a

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-R-GECO1

Plasmid

#113943

Purpose

Bacterial expression of red fluorescent Ca2+ sensor R-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

R-GECO1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pBAD-CAR-GECO1

Plasmid

#113939

Purpose

Bacterial expression of red fluorescent Ca2+ sensor CAR-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

CAR-GECO1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1s-3NLS-P2A-EGFP-3NLS

Plasmid

#217478

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1s

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1s

Use

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-CMV-Nier1b-3NLS-P2A-EGFP-3NLS

Plasmid

#217479

Purpose

Mammalian expression plasmid for far-red fluorescent Ca2+ indicator Nier1b

Depositor

Peng Zou

Article

Lin et al Cell Rep Methods. 2023 Jul 27;3(8):10054

Insert

Nier1b

Use

Tags

3xNLS, P2A-EGFP-3xNLS

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 22, 2024

Availability

Academic Institutions and Nonprofits only

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pRSM41

Plasmid

#113940

Purpose

Constitutive bacterial expression of red fluorescent Ca2+ sensor REX-GECO1

Depositor

Thomas Hughes, Robert Campbell

Article

Molina et al Biophys J. 2019 May 21;116(10):1873-1886

Insert

REX-GECO1

Use

Tags

6xHis

Expression

Bacterial

Mutation

Promoter

Available since

Dec. 3, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pGL3-NFAT luciferase

Plasmid

#17870

Purpose

3x NFAT binding sequence upstream of a luciferase reporter; can be used to assay signal transduction through Ca2+, calcineurin and NFAT in any tissue

Depositor

Jerry Crabtree

Article

Clipstone et al Nature. 1992 Jun 25. 357(6380):695-7.

Insert

3x NFAT binding sequence (IL2 )

Use

Luciferase

Tags

IL2 promoter and Luciferase

Expression

Mammalian

Mutation

Promoter

IL-2 promoter

Available since

April 29, 2008

Availability

Academic Institutions and Nonprofits only

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5HT6-mCherry-G-GECO1.0

Plasmid

#47500

Purpose

Fluorescent reporter for Ca2+ inside primary cilia with mCherry reference

Depositor

Takanari Inoue

Article

Su et al Nat Methods. 2013 Sep 22. doi: 10.1038/n

Insert

G-GECO1.0

Use

Tags

5HT6 and mCherry

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pCAG-CalfluxCTN

Plasmid

#190068

Purpose

Expresses BRET Ca2+ sensor CalfluxCTN in mammalian cells

Depositor

Carl Johnson

Article

Cumberbatch et al Sci Signal. 2022 Aug 16;15(747):eabq7618

Insert

CalfluxCTN

Use

Tags

Expression

Mammalian

Mutation

Promoter

CAG promoter

Available since

Sept. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pFSW GAP43-jRGECO1a P2A Syp-Halo

Plasmid

#185565

Purpose

Expression of axonally-targeted jRGECO1a, a red Ca2+ sensor, along with synaptophysin-HaloTag in neuronal cells, separated by a P2A sequence

Depositor

Edwin Chapman

Article

Bradberry et al J Physiol. 2022 Feb;600(3):645-654. doi:

Insert

GAP43-jRGECO1a P2A Syp-Halo

Use

Lentiviral

Tags

HaloTag

Expression

Mutation

Promoter

Available since

Oct. 20, 2022

Availability

Academic Institutions and Nonprofits only

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hBACCS2

Plasmid

#67625

Purpose

Mammalian expression of hBACCS2, a human blue light-activated Ca2+ channel switch

Depositor

Takao Nakata

Article

Ishii et al Nat Commun. 2015 Aug 18;6:8021. doi: 10.

Insert

hBACCS2

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 21, 2015

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Na0.5

Plasmid

#198634

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Na0.5

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pTorPE-Y-GECO2f

Plasmid

#105065

Purpose

A yellow fluorescent Ca2+ indicator variant with low binding affinity and fast kinetics for expression in bacterial

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2f

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

April 9, 2018

Availability

Academic Institutions and Nonprofits only

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CMV-Y-GECO2m

Plasmid

#105070

Purpose

A yellow fluorescent Ca2+ indicator variant with medium binding affinity and kinetics for expression in mammalian cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2m

Use

Tags

6x His tag

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 11, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pTorPE-Y-GECO2.2s

Plasmid

#105069

Purpose

A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

Depositor

Robert Campbell

Article

Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

Insert

Y-GECO2.2s

Use

Tags

6x His tag

Expression

Bacterial

Mutation

Promoter

pBAD

Available since

March 22, 2018

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-145-146

Plasmid

#198600

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-145-146

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-HaloGFP-169-170

Plasmid

#198612

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-169-170

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-HaloGFP-Ca0.5

Plasmid

#198632

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca0.5

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pBad-HaloGFP-Ca0.2

Plasmid

#198631

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca0.2

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pBad-HaloGFP-Ca1

Plasmid

#198633

Purpose

Green fluorescent Ca2+ ion indicator for bacterial expression.

Depositor

Robert Campbell

Article

Zhu et al Nat Chem Biol. 2023 Jan;19(1):38-44. doi

Insert

HaloGFP-Ca1

Use

Tags

Expression

Bacterial

Mutation

Promoter

araBAD promoter

Available since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge