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Plasmid#216503PurposeRas activity dependent proximity labeler, Key only, untargetedDepositorInsertRas-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Ras-LOCKR-PL Cage
Plasmid#216502PurposeRas activity dependent proximity labeler, Cage only, untargetedDepositorInsertRas-LOCKR-PL Cage
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 9, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PM-Ras-LOCKR-PL Key
Plasmid#216504PurposeRas activity dependent proximity labeler, Key only, plasma membrane localizedDepositorInsertPM-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Golgi-Ras-LOCKR-PL Key
Plasmid#216505PurposeRas activity dependent proximity labeler, Key only, Golgi localizedDepositorInsertGolgi-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 ER-Ras-LOCKR-PL Key
Plasmid#216506PurposeRas activity dependent proximity labeler, Key only, ER localizedDepositorInsertER-Ras-LOCKR-PL Key
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 Mito-Ras-LOCKR-PL Key
Plasmid#216507PurposeRas activity dependent proximity labeler, Key only, mitochondria localizedDepositorInsertMito-Ras-LOCKR-PL
UseTagsExpressionMammalianMutationPromoterAvailable sinceApril 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
FB395
Plasmid#203631PurposeTU for the expression of luciferase under a synthetic promoter containing the target sequence for gRNA1 (1xLuc).DepositorInsertR1:G1aG2b.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB396
Plasmid#203632PurposeTU for the expression of luciferase under a synthetic promoter containing two copies of the target sequence for gRNA1 (2xLuc).DepositorInsertR1:G1ab.1:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
FB397
Plasmid#203633PurposeTU for the expression of luciferase under a synthetic promoter containing three copies of the target sequence for gRNA1 (3xLuc).DepositorInsertR1:G1abc.3:mPAF:Luc:TtrpC
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceSept. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
LV TRE-GFP-TMD-DLL1-Nbox-mutant
Plasmid#194937PurposeLV construct encoding a mutated GFP-TMD-DLL1 ligand containing an ablated Nbox site under dox-inducible TRE promoter and containing a PGK-rtTA-M2-IRES-Puro regulator/resistance marker cassette.DepositorInserteGFP-(PDGFR)TMD-rDLL1 ICD-Nbox-AlaX3-mutant
UseLentiviralTagsExpressionMutationPromoterAvailable sinceMarch 3, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL714
Plasmid#140384PurposeExpresses CtcS-regulated 3WJdB RNA aptamer driven by T7 promoter with the ctcO sequence 5BP downstream from the promoterDepositorInsertT7-ctcO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 4, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL720
Plasmid#140390PurposeExpresses QacR-regulated 3WJdB RNA aptamer driven by T7 promoter with the qacO sequence 5BP downstream from the promoterDepositorInsertT7-qacO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJBL722
Plasmid#140392PurposeExpresses TtgR-regulated 3WJdB RNA aptamer driven by T7 promoter with the ttgO sequence 5BP downstream from the promoterDepositorInsertT7-ttgO-3WJdB-T
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
YIP sHRPa-Aga1P
Plasmid#73151PurposeLarge fragment of split HRP fused to the N-terminus of the yeast mating protein Aga1P. When linearized, this vector incorporates into the yeast genome.DepositorInsertsHRPa-Aga1P
UseTagsV5 tagExpressionYeastMutationPromoterglyceraldehyde-3-phosphate dehydrogenase GPD prom…Available sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
mEGFP-NbALFA
Plasmid#159986PurposeFor the expresson and purification of the EGFP-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmEGFP-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mEGFP and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
mCherry-NbALFA
Plasmid#159987PurposeFor the expresson and purification of the mCherry-tagged anti-ALFA tag nanobody (NbALFA)DepositorInsertmCherry-NbALFA
UseTags8histidine-HRV3C protease cleavage siteExpressionBacterialMutation3GS linker between mCherry and NbALFAPromoterAvailable sinceOct. 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
CAG sHRPb-FKBP-post mGRASP
Plasmid#73146PurposeSmall fragment of split HRP and FKBP fused to the extracellular terminus of the "post mGRASP" scaffold published by Magee, in which the majority of the NLG1 extracellular domain is deleted.DepositorInsertsHRPb-FKBP-post mGRASP
UseTagsHA tagExpressionMammalianMutationPromoterCAGAvailable sinceJune 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCTCON2 Aga2P-HRP
Plasmid#73152PurposeFull-length horseradish peroxidase (HRP) fused to the C-terminus of the yeast mating protein Aga2P. Galactose-inducible expression.DepositorInsertAga2P-HRP
UseTagsHA tag and myc tagExpressionYeastMutationPromoterGAL1Available sinceJune 21, 2016AvailabilityAcademic Institutions and Nonprofits only -
pNCS mOrange
Plasmid#91764PurposeBacterial expression of a fluorescent protein, monomeric Orange (mOrange)DepositorInsertmonomeric Orange
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 9, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNCS tdTomato
Plasmid#91767PurposeBacterial expression of a fluorescent protein, tandem dimer Tomato (tdTomato)DepositorInserttandem dimer Tomato
UseTagsHexa-Histidine tag, Xpress epitope for detection …ExpressionBacterialMutationPromoterT7Available sinceJune 27, 2017AvailabilityAcademic Institutions and Nonprofits only