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wt Cus2p
Plasmid#73046PurposeWild-type S. cerevisiae Cus2p gene for recombinant expression and crystallizationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationPromoterT7Available sinceMay 14, 2019AvailabilityAcademic Institutions and Nonprofits only -
L284F Cus2p
Plasmid#73059PurposeS. cerevisiae Cus2p gene with L284F MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationLeucine 284 to PhenylalaninePromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
D282N Cus2p
Plasmid#73060PurposeS. cerevisiae Cus2p gene with D282N MutationDepositorArticleInsertCUS2p (CUS2 Budding Yeast)
UseTags6x His tag fused to TEV cleavage siteExpressionBacterial and YeastMutationAsp 282 to AsnPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pCRII-Topo S100a10 in situ probe
Plasmid#45603DepositorInserts100a10 in situ probe (S100a10 Mouse)
UseIn situTagsExpressionMutationfragment contains bp# 21-275 of BC025044PromoterAvailable sinceJuly 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
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pFAB3869
Plasmid#47842PurposeBIOFAB RFP reporter plasmid for measuring promoter 11 + BCD1 efficiency.DepositorInsertpromoter 11 and BCD1
UseSynthetic BiologyTagsExpressionBacterialMutationPromotersee insertAvailable sinceAug. 29, 2013AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CB
Plasmid#12526DepositorInsertPIK3CB (PIK3CB Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro Myr HA PIK3CB
Plasmid#12527DepositorInsertPIK3CB (PIK3CB Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationMyristoylation. Constitutively active allele.PromoterAvailable sinceNov. 25, 2019AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro Myr HA PIK3CA
Plasmid#12523DepositorInsertPIK3CA Myr (PIK3CA Human)
UseRetroviralTagsHA and MyrExpressionMammalianMutationMyristoylation. Constitutively active allele; I14…PromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA
Plasmid#12522PurposeRetroviral expression of HA tagged phosphoinositide 3-kinaseDepositorInsertPIK3CA (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA E545K
Plasmid#12525DepositorInsertPIK3CA E545K (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationE545K. Tumor-derived allele.PromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
pBabe puro HA PIK3CA H1047R
Plasmid#12524PurposeRetroviral expression of HA tagged phosphoinositide 3-kinase H1047R (Tumor-derived allele)DepositorInsertPIK3CA H1047R (PIK3CA Human)
UseRetroviralTagsHAExpressionMammalianMutationH1047R. Tumor-derived allele.PromoterAvailable sinceAug. 18, 2006AvailabilityAcademic Institutions and Nonprofits only -
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5JJX (SART3)
Plasmid#101879PurposeBacterial expression for structure determination; may not be full ORFDepositorInsertSART3 (SART3 Human)
UseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
5JJW (USP15_SART3)
Plasmid#101880PurposeBacterial expression for structure determination; may not be full ORFDepositorUseTags6xHis, TEVExpressionBacterialMutationPromoterT7Available sinceApril 30, 2018AvailabilityIndustry, Academic Institutions, and Nonprofits -
pFL-EGFP-3xFlag-HLTF
Plasmid#193055PurposeBaculovirus expression of human HLTF for recombinant protein productionDepositorInsertHLTF (HLTF Human)
UseTags3xFlagExpressionInsectMutationPromoterpolyhedronAvailable sinceFeb. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B
Plasmid#133308PurposeExpression of luciferase driven by eIF3B promoter regionDepositorInserteIF3B promoter (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pGL4-eIF3B MBE3 mutant
Plasmid#133310PurposeExpression of luciferase driven by eIF3B promoter region mutated at the E-box binding motif 3DepositorInserteIF3B promoter MBE3 mutant (EIF3B Human)
UseLuciferaseTagsExpressionMammalianMutationccacgtgacc changed to cAaAAAAaccPromotereIF3BAvailable sinceOct. 28, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCZGY3163
Plasmid#101248PurposeGFP11::rps-18, ribosomal protein subunit, bound to C-terminal part of GFP. Shows GFP localized to ribosomes when combined with GFP 1-10.DepositorInsertgfp11::rps-18 (rps-18 Synthetic, Nematode)
UseGatewayTagsGFP11ExpressionMutationPromoterAvailable sinceSept. 29, 2017AvailabilityAcademic Institutions and Nonprofits only
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