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pNB200
Plasmid#169111PurposeTESEC backbone for target expressionDepositorInsertlacZ (lacZ Escherichia coli K12 MG1655)
UseSynthetic BiologyTagsExpressionBacterialMutationCodon optimized for E. coliPromoterAvailable sinceJuly 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
EcMscL
Plasmid#92418PurposeE. coli Mechanosensitive Channel of Large ConductanceDepositorInsertMscL (mscL E. coli)
UseTags6x Histidine Tag and Thrombin Cleavage Site betwe…ExpressionBacterialMutationPromoterT7 PromoterAvailable sinceJuly 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (KmR)
Plasmid#177207PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterSynthetic constitutive promoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
p416-GPD-HA-PPK
Plasmid#183943PurposeExpresses HA-tagged E. coli PPK from yeast GPD promoterDepositorInsertPPK (ppk E. coli)
UseTagsHAExpressionYeastMutationWTPromoterGPDAvailable sinceJune 2, 2022AvailabilityAcademic Institutions and Nonprofits only -
pmr101A-cPr-mCherry (ErmR)
Plasmid#177206PurposePlasmid with p15A origin for constitutive mCherry expression in E. coliDepositorInsertmCherry
UseTagsExpressionBacterialMutationPromoterSynthetic constitutive promoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAIP4_1TJE
Plasmid#234203PurposeHeterologous protein expression of threonine--tRNA ligase (EC 6.1.1.3) (Threonyl-tRNA synthetase) in Escherichia coliDepositorInsert1TJE
UseTags10x HisTag-Smt3ExpressionBacterialMutationPromoterT7Available sinceMarch 6, 2025AvailabilityIndustry, Academic Institutions, and Nonprofits -
p416-GPD-PPK(mutant)
Plasmid#183942PurposeExpresses catalytic mutant of E. coli PPK from yeast GPD promoterDepositorInsertPPK(mutant) (ppk E. coli )
UseTagsExpressionYeastMutationmutations in His435, His454, and His592 to AlaninePromoterGPDAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK649
Plasmid#72399PurposeProduces Escherichia coli phosphoribosylpyrophosphate amidotransferase (EcPurF)DepositorInsertamidophosphoribosyltransferase
UseTagsExpressionBacterialMutationPromoterT7Available sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-zwf
Plasmid#65825Purposezwf targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertzwf guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceSept. 22, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-udhA
Plasmid#65818PurposeudhA targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertudhA guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceAug. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCASCADE-gltA2
Plasmid#65817PurposegltA targeting guide RNA E. coli , Low Phosphate InductionDepositorInsertgltA2 guide
UseTagsExpressionBacterialMutationPromoterE . coli ugpB gene promoter, low phosphate induc…Available sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
pHL1914
Plasmid#53042PurposePconNoHindM2:hfqlead::gfp, PconNoHind:RBS(st3):tetRDepositorInsertsGFP
tetR
UseSynthetic BiologyTagsRBS(st3) and hfqleadExpressionBacterialMutationPromoterPconNoHindM2 and pLlacO-1Available sinceMay 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCK302
Plasmid#87768PurposeAs pCK301 (E. coli rhaBAD promoter upstream of sfGFP, sfGFP can be replaced with any gene of interest), but rhaS cloned downstream of ampR, which allows non-metabolisable inducer L-mannose to be usedDepositorInsertsPrhaBAD-sfGFP
rhaS from E. coli with its native RBS from E. coli
UseSynthetic BiologyTags6xHis TagExpressionBacterialMutationPromoterPrhaBAD rhamnose-inducible promoter from E. coli …Available sinceMay 1, 2017AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_R166QTS
Plasmid#191298PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, R166Q TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pKDsgRNA-FRT
Plasmid#183241Purposeanhydrotetracycline (aTC) inducible sgRNA that targets FRT scar sites and arabinose inducible lambda RedDepositorInsertsgRNA-FRT
UseTagsExpressionBacterialMutationPromoterP-tetAvailable sinceApril 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBB541
Plasmid#27394DepositorUseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 13, 2012AvailabilityAcademic Institutions and Nonprofits only -
pMBIS
Plasmid#17817DepositorInsertmevalonate kinase, phosphomevalonate kinase, mevalonate pyrophosphate decarboxylatse, IPP isomerase, FPP synthase
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterAvailable sinceJuly 27, 2008AvailabilityAcademic Institutions and Nonprofits only -
psgRNA
Plasmid#114005Purposeexpress sgRNA. ColE1, KanDepositorInsertgRNA
UseTagsExpressionMutationPromoterBBa_J23119Available sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only -
psgRNAc
Plasmid#114006Purposeexpress sgRNA, p15A, CRMDepositorInsertgRNA
UseTagsExpressionMutationPromoterBBa_J23119Available sinceFeb. 11, 2019AvailabilityAcademic Institutions and Nonprofits only
