1. p426 103Q GPD

   Plasmid

   #1184

   Depositor

   Susan Lindquist

   Article

   Krobitsch et al Proc Natl Acad Sci U S A 2000 Feb 15;97(

   Insert

   htt 103Q (HTT Human)
   

   Use

   Tags

   GFP

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Availability

   Academic Institutions and Nonprofits only

These results may also be relevant.

1. GPR180-COMP-blac-flag-his

   Plasmid

   #110955

   Purpose

   Expresses pentamerised full-length protein ectodomain with no N-linked glycans in mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag, COMP pentamerisation domain, flag- and 6xHis tags.

   Depositor

   Gavin Wright

   Article

   Knockel et al Mol Cell Proteomics. 2021;20:100038. doi

   Insert

   PF3D7_1213500 (PF3D7_1213500 )
   

   Use

   Tags

   Exogenous signal peptide of mouse origin and rat …

   Expression

   Mammalian

   Mutation

   All NXT/S sites mutated to NXA, where X is any am…

   Promoter

   CMV

   Available since

   Sept. 28, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pDONR223-ABL2

   Plasmid

   #23909

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   ABL2 (ABL2 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   K115M

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

3. pDONR223-MAP4K1

   Plasmid

   #23484

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   MAP4K1 (MAP4K1 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

4. pNLF1-C_EIF2D:1-584

   Plasmid

   #211085

   Purpose

   Mammalian expression of full-length human EIF2D. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   EIF2D:M1-K584 (EIF2D Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pNLF1-C_EXOSC6:1-272

   Plasmid

   #211084

   Purpose

   Mammalian expression of full-length human EXOSC6. C-terminal NanoLuc luciferase tag.

   Depositor

   Cheryl Arrowsmith

   Article

   Ackloo et al bioRxiv 2024.03.03.583197

   Insert

   EXOSC6:M1-P272 (EXOSC6 Human)
   

   Use

   Tags

   NanoLuc luciferase

   Expression

   Mammalian

   Mutation

   wild type

   Promoter

   CMV

   Available since

   March 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pDONR223-DKFZp761P0423

   Plasmid

   #23475

   Depositor

   David Root, William Hahn

   Article

   Johannessen et al Nature. 2010 Dec 16;468(7326):968-72. do

   Insert

   DKFZp761P0423 (SGK223 Human)
   

   Use

   Gateway donor vector

   Tags

   Expression

   Mutation

   GS inserted after aa343, del aa1068-1184

   Promoter

   Available since

   July 30, 2010

   Availability

   Academic Institutions and Nonprofits only

7. p3E_he1a:mCherry

   Plasmid

   #113881

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   mCherry

   Expression

   Mutation

   Promoter

   Available since

   Sept. 27, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. p3E_he1a:eYFP

   Plasmid

   #113879

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   eYFP

   Expression

   Mutation

   Promoter

   Available since

   Sept. 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. p3E_he1a:ECFP

   Plasmid

   #113880

   Purpose

   p3E entry vector for Three Multisite Gateway

   Depositor

   Marnie Halpern

   Insert

   he1a (he1.1 Zebrafish)
   

   Use

   Zebrafish danio expression

   Tags

   ECFP

   Expression

   Mutation

   Promoter

   Available since

   Jan. 24, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pLV-Enh eGFP Reporter-muKC2-INT2

    Plasmid

    #59287

    Purpose

    Lentiviral GFP reporter for testing enhancer activity in different cell types. Insert is an enhancer identified in the intronic region of the keratin type II cluster.

    Depositor

    Benjamin Yu

    Insert

    krt80-Intron 4
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Mu Hsp68 minimal promoter

    Available since

    Oct. 1, 2014

    Availability

    Academic Institutions and Nonprofits only

11. pSP64-Vasa-GFP

    Plasmid

    #135595

    Purpose

    Expression of Vasa-GFP mRNA for microinjection for visualization of Vasa protein dynamics in S. purpuratus

    Depositor

    Mamiko Yajima

    Article

    Yajima et al Development. 2015 Jun 1;142(11):1960-70.

    Insert

    Vasa-GFP
    

    Use

    In vitro transcription (mrna synthesis)

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    SP6

    Available since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pT2KXIGdeltaIn-MCS-birA-tagRFP

    Plasmid

    #58378

    Purpose

    tol2 transgenesis for biotin based TRAP - BirA ligase

    Depositor

    Didier Stainier

    Article

    Housley et al Development. 2014 Oct;141(20):3988-93. d

    Insert

    birA tagRFP
    

    Use

    Tags

    tagRFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pT2KXIGdeltaIn-MCS-birA-EGFP

    Plasmid

    #58377

    Purpose

    tol2 transgenesis for biotin based TRAP - BirA ligase

    Depositor

    Didier Stainier

    Article

    Housley et al Development. 2014 Oct;141(20):3988-93. d

    Insert

    birA EGFP
    

    Use

    Tags

    EGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pFA6a-link-ymScarletI-URA3

    Plasmid

    #168055

    Purpose

    Replaces Addgene plasmid 118457. Yeast tagging vector to create a gene fusion with yeast optimized mScarletI with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymScarletI
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pFA6a-link-ymTq2-URA3

    Plasmid

    #168057

    Purpose

    Replaces Addgene Plasmid 118458. Yeast tagging vector to create a gene fusion with yeast optimized mTurquoise2 with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymTq2
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pFA6a-link-ymYPET-URA3

    Plasmid

    #168056

    Purpose

    Replaces Addgene plasmid 118460. Yeast tagging vector to create a gene fusion with yeast optimized mYPET with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymYPET
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pFA6a-link-ytdTomato-URA3

    Plasmid

    #168054

    Purpose

    Replaces Addgene plasmid 118461. Yeast tagging vector to create a gene fusion with yeast optimized tdTomato with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ytdTomato
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pT2KXIGdeltaIn-MCS-Avi-EGFP-rpl10a

    Plasmid

    #58380

    Purpose

    tol2 transgenesis for biotin based TRAP - Avi tagged Ribosome

    Depositor

    Didier Stainier

    Article

    Housley et al Development. 2014 Oct;141(20):3988-93. d

    Insert

    avi-EGFP-rpl10a
    

    Use

    Tags

    Avi Tag and EGFP

    Expression

    Mutation

    Promoter

    Available since

    Aug. 4, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pFA6a-link-ymVenus-URA3

    Plasmid

    #168058

    Purpose

    Replaces Addgene plasmid 118459. Yeast tagging vector to create a gene fusion with yeast optimized mVenus with CaUra3 selection; Contains the linker region at the 5’ end of the FP

    Depositor

    Bas Teusink

    Article

    Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

    Insert

    ymVenus
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    June 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pCDNA3-ySet2

    Plasmid

    #51698

    Purpose

    Expresses full length yeast Set 2 gene in a mamalian expression vector

    Depositor

    Tom Misteli

    Article

    Luco et al Science. 2010 Feb 19;327(5968):996-1000.

    Insert

    yeast Set2 (SET2 Budding Yeast)
    

    Use

    Tags

    C-terminal FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    April 16, 2014

    Availability

    Academic Institutions and Nonprofits only