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Showing: 901 - 950 of 23234 results

  2. pEVOL-pAzF

    Plasmid
    #31186
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-azido-l-phenylalanine, into proteins in E coli response to the amber codon, TAG
    Depositor
    Peter Schultz
    Article
    Chin et al J Am Chem Soc. 2002 Aug 7;124(31):9026-7
    Insert
    M.j. p-azidohenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32T, E107N, D158P, I159L, L162Q, D286R
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31186-plasmid-map-sequence-id-334781

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  3. pCVD437

    Plasmid
    #63758
    Purpose
    7 Kb Bg1II I fragment of pCVD 436 cloned into BamHI site of pTTQ181 (incorrect orientation for expression off of pTTQ181 promoter)
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 21, 2015
    Availability
    Academic Institutions and Nonprofits only

  4. pEVOL-pBpF

    Plasmid
    #31190
    Purpose
    tRNA synthetase/tRNA pair for the in vivo incorporation of a photocrosslinker, p-benzoyl-l-phenylalanine into proteins in E coli in response to the amber codon, TAG.
    Depositor
    Peter Schultz
    Article
    Chin et al Proc Natl Acad Sci U S A. 2002 Aug 20;99
    Insert
    M.j. p-benzoylphenylalanine RS (2 copies +tRNA)
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Y32G, E107P, D158T, I159S
    Promoter
    Available since
    Dec. 9, 2011
    Availability
    Academic Institutions and Nonprofits only
    31190-plasmid-map-sequence-id-418765

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  6. pUAS-kkv-R896K::NG

    Plasmid
    #138957
    Purpose
    Used to generate a transgene that will express an inactive fluorescent Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv-R896K::NG (kkv Fly)
    Use
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Mutation
    R896K
    Promoter
    UAS
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138957-plasmid-map-sequence-id-274787

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  7. pUAS-kkv-OH

    Plasmid
    #138956
    Purpose
    Used to generate a transgene that will express an epitope tagged Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv with C terminal epitope tag (kkv Fly)
    Use
    Tags
    ollas and his-6 added to C terminus of protein co…
    Expression
    Insect
    Mutation
    Promoter
    UAS
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138956-plasmid-map-sequence-id-274764

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  8. pHD-DsRed-kkv-3::NG

    Plasmid
    #138960
    Purpose
    Used to edit the endogenous Drosophila kkv gene to encode a fluorescent Chitin Synthase.
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    3' end of kkv and neon green (kkv Fly)
    Use
    An editing vector for drosophila
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Mutation
    Promoter
    Available since
    Sept. 27, 2023
    Availability
    Academic Institutions and Nonprofits only
    138960-plasmid-map-sequence-id-274768

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  9. pMis1_1B

    Plasmid
    #192079
    Purpose
    A pBBR-1 based vector with IncP group plasmid compatibility for expression in Methylorubrum extorquens using rhamnose dependent induction
    Depositor
    Markus Buchhaupt
    Article
    Poschel et al Microbiologyopen. 2022 Oct;11(5):e1325.
    Inserts
    KanR
    rhaR
    rhaS
    pBBR1 rep
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    Dec. 15, 2022
    Availability
    Academic Institutions and Nonprofits only
    192079-plasmid-map-sequence-id-380008

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  12. pcDNA3.1_ spike_del19

    Plasmid
    #155297
    Purpose
    Expression of spike with C-term deletion of 19 aa, used to generate high efficiency SARS-CoV-2-pseudotyped lentiviral particles
    Depositor
    Raffaele De Francesco
    Article
    Conforti et al Mol Ther. 2021 Sep 20. pii: S1525-0016(2
    Insert
    SARS-Cov2 spike_deleted (S SARS-Cov2)
    Use
    Generation of sars-cov-2-pseudotyped lentiviral p…
    Tags
    Expression
    Mutation
    Deletion in C-term 19 aa of spike
    Promoter
    CMV
    Available since
    Aug. 5, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    155297-plasmid-map-sequence-id-307858

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  18. E3_pENTR_L3-L2_NI_G1333-DddA11-N

    Plasmid
    #191609
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191609-plasmid-map-sequence-id-379112

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  19. E3_pENTR_L3-L2_NN_G1333-DddA11-N

    Plasmid
    #191610
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191610-plasmid-map-sequence-id-379111

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  20. E1_pENTR_L1-L4_HD_G1333-DddA11-C

    Plasmid
    #191599
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191599-plasmid-map-sequence-id-379206

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  21. E1_pENTR_L1-L4_NG_G1333-DddA11-C

    Plasmid
    #191600
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191600-plasmid-map-sequence-id-379205

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  22. E1_pENTR_L1-L4_NI_G1333-DddA11-C

    Plasmid
    #191601
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191601-plasmid-map-sequence-id-379204

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  23. E1_pENTR_L1-L4_NN_G1333-DddA11-C

    Plasmid
    #191602
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL1 and L4
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191602-plasmid-map-sequence-id-379199

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  24. E3_pENTR_L3-L2_HD_G1333-DddA11-N

    Plasmid
    #191607
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191607-plasmid-map-sequence-id-379194

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  25. E3_pENTR_L3-L2_NG_G1333-DddA11-N

    Plasmid
    #191608
    Purpose
    ptpTALECD_v2mod vector assembly, Entry clone 1 with attL2 and L3
    Depositor
    Shin-ichi Arimura
    Insert
    C- and N termini of platinum TALEN, a half of the evolved DddA (DddA11), and a uracil glycosylase inhibitor
    Use
    Other
    Tags
    Expression
    Mutation
    Promoter
    Available since
    Feb. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    191608-plasmid-map-sequence-id-379113

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  26. pET-Impact-T4gp32(L301G)

    Plasmid
    #228209
    Purpose
    Expression in BL21(DE3) of single strand DNA binding protein L301G
    Depositor
    Stephen Benkovic
    Insert
    61
    Use
    Tags
    Sce VMA intein-chitin binding domain
    Expression
    Bacterial
    Mutation
    Mutated leucine 301 to glycine (L301G), improves …
    Promoter
    T7
    Available since
    March 6, 2025
    Availability
    Academic Institutions and Nonprofits only
    228209-plasmid-map-sequence-id-453946

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  32. pUAS-kkv::NG

    Plasmid
    #138953
    Purpose
    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv (NEWENTRY Fly)
    Use
    Uas transgene vector
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Mutation
    Promoter
    Available since
    Nov. 1, 2020
    Availability
    Academic Institutions and Nonprofits only
    138953-plasmid-map-sequence-id-274788

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  36. pCVD438

    Plasmid
    #63759
    Purpose
    eae gene (3476 bp) from pJY21 removed by SamI/SphI digestion, cloned into EcoRV/SphI sites of pACYC184. Insertional inactivation of tetracycline gene.
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  39. pCVD434

    Plasmid
    #63757
    Purpose
    EPEC eae probe. 1.1 kb Sa1I/StuI fragment of E2348/69 (from gene bank cosmid II E1) cloned into Sa1I/SmaI site of pUC18.
    Depositor
    James Kaper
    Article
    Jerse et al Proc Natl Acad Sci U S A. 1990 Oct;87(20
    Insert
    eae probe
    Use
    Tags
    Expression
    Bacterial
    Mutation
    Promoter
    Available since
    April 6, 2015
    Availability
    Academic Institutions and Nonprofits only

  43. pUAS-kkv-mos::NG

    Plasmid
    #138958
    Purpose
    Used to generate a transgene that will express a fluorescent Chitin Synthase in Drosophila where the active site is converted to the mosquito sequence. Expression by using Gal4
    Depositor
    Paul Adler
    Article
    Adler et al bioRxiv 2020
    Insert
    kkv (NEWENTRY Fly)
    Use
    Uas transgene vector
    Tags
    Neon Green fused to C terminus of Kkv
    Expression
    Insect
    Mutation
    8 bp that are in the region that encodes the cata…
    Promoter
    UAS
    Available since
    Feb. 17, 2023
    Availability
    Academic Institutions and Nonprofits only
    138958-plasmid-map-sequence-id-274779

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Showing: 901 - 950 of 23234 results