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Plasmid#175801PurposePlasmid for overexpression of recombinant AviTagged, His-tagged fusion protein eGFP-DspB(E184Q W330Y), used as a non-binding control for detection of biofilm polysaccharide PNAG.DepositorInsertDispersin B
UseTagsAviTag, Hexahistidine tag, and eGFPExpressionBacterialMutationE184Q W330YPromoterT7Available sinceOct. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-Coh-ddFLN4-ELP(MV7E2)3-His-ybbR
Plasmid#157672PurposeExpression of Coh (type III from Ruminococcus flavefaciens) with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertCohesin (R.f) with ddFLN4, ELP, Histag and ybbR tag
UseTagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialMutationPromoterT7Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-His-ELP(MV7E2)3-ddFLN4-SpyCatcher
Plasmid#157674PurposeExpression of SpyCatcher with ddFLN4, ELP linker, His6x tag, and ybbR Tag.DepositorInsertSpyCatcher with ddFLN4, ELP, Histag and ybbR tag
UseTagsddFLN4, ELP, Histag and ybbR tagExpressionBacterialMutationPromoterT7Available sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (BMB-KO, S199AzF)-HIS
Plasmid#153445PurposeE. coli expression (amber suppression) of Rc. XDocB binding mode B knock-out mutant with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
UseTags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG). Argi…PromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbr-XMod-Doc (BMA-KO, S199AzF)-HIS
Plasmid#153444PurposeE. coli expression (amber suppression) of Rc. XDocB binding mode A knock-out mutant with serine at position 199 replaced with amber codon.DepositorInsertRc.XDocB
UseTags6xHis and ybbr tagExpressionBacterialMutationSerine 199 was mutated to amber codon (TAG). Argi…PromoterT7 promoterAvailable sinceJuly 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pET-GST-TNRC6A-(935-984)-NES-mut
Plasmid#42051DepositorInsertTNRC6A (TNRC6A Human)
UseTagsGST and HisExpressionBacterialMutationcontain aa935-984, F951A, F955A, I958A, F960APromoterAvailable sinceApril 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
M61 pPET28c-Xenopus beta catenin domains R10C
Plasmid#17202DepositorInsertbeta catenin (ctnnb1.L Frog)
UseTagsHISExpressionBacterialMutationdomains R10CPromoterAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
M60 pPET28a-Xenopus beta catenin domains NR7
Plasmid#17201DepositorInsertbeta catenin (ctnnb1.L Frog)
UseTagsHISExpressionBacterialMutationdomains NR7PromoterAvailable sinceSept. 12, 2012AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 AD (325-645, C643A, Δ 368-492)/pET28HMT
Plasmid#111072PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 AD (325-645, C643A, Δ 368-492)PromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
M57 pPET28a-TEV-full-length human beta catenin
Plasmid#17198DepositorInsertbeta catenin (CTNNB1 Human)
UseTagsHISExpressionBacterialMutationPromoterAvailable sinceFeb. 1, 2008AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mCherry LIC cloning vector (MBP-mCherry)
Plasmid#29747DepositorTypeEmpty backboneUseTagsBiotin - His6 - MBP -TEV and mCherryExpressionBacterialMutationPromoterAvailable sinceSept. 22, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 MBP prescission LIC cloning vector (HMPKS)
Plasmid#29721DepositorTypeEmpty backboneUseTagsHis6-MBP-prescissionExpressionBacterialMutationPromoterAvailable sinceSept. 17, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28a-HIS-TagGFP2-TEV-GGG-ELP(120nm)-Cys
Plasmid#90473PurposeElastin Like Polypeptide: [(VPGEG)-(VPGVG)4-(VPGAG)2-(VPGGG)2-(VPGEG)]6, with N-Term TagGFP2, and a TEV-cleavage site, leaving an N-terminal Sortase Recognition Sequence GGG and a C-terminal CysteineDepositorInsertTagGFP2-TEV-GGG-ELP(120nm)-Cys
UseTagsCysteine and Sortase-TagExpressionBacterialMutationPromoterT7Available sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pAMS823 His6-MBP-3C-ORF2p (238-1061, ORFeus-Hs) in pET41
Plasmid#213024PurposeExpresses human LINE-1 ORF2p Core (238-1061) in E. coli as an N-MBP fusionDepositorInsertLINE-1 ORF2p (238-1061)
UseTagsHis6-MBP-3CExpressionBacterialMutationPromoterT7 / LacAvailable sinceMay 6, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET E. coli expression vector with BioBrick polypromoter restriction sites (14-A)
Plasmid#48307DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceNov. 5, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET His6 GFP TEV LIC cloning vector (1GFP)
Plasmid#29663DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHis6-GFP-TEVExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP mRuby2 LIC cloning vector (MBP-mRuby2)
Plasmid#86933PurposepET LIC cloning vector for Biotin-His6-MBP-N10-tev-yORF-mRuby2DepositorTypeEmpty backboneUseTagsBiotin, His6, MBP, Tev Recognition Sequence, and …ExpressionBacterialMutationPromoterT7Available sinceMarch 28, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET Biotin His6 mCherry LIC cloning vector (H6-mCherry)
Plasmid#29722DepositorTypeEmpty backboneUseTagsBiotin - His6 - TEV and mCherryExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET MBP GFP LIC cloning vector (MBP-msfGFP)
Plasmid#29750DepositorTypeEmpty backboneUseTagsBiotin - His6 - MBP -TEV and GFPExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET28 MBP NAAEF-AMPKa1 (13-476,529-550)-b2(76-272)-g1(24-327)
Plasmid#177850PurposeBacterial Expression of AMPKDepositorInsertAMPK (a1b2g1)
UseTagsMBPExpressionBacterialMutationPromoterAvailable sinceDec. 9, 2021AvailabilityAcademic Institutions and Nonprofits only