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We narrowed to 13,821 results for: pET

Showing: 3761 - 3780 of 13821 results

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AAV-Synapsin-NLS-YPET-optoNES-WPRE

Plasmid

#159942

Purpose

optical regulation of nuclear export

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

synthetic

Promoter

hSyn1

Available since

Oct. 23, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-YPET-optoNES.F434L-WPRE

Plasmid

#159975

Purpose

optical regulation of nuclear export with shorter relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.F434L

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

F434L

Promoter

hSyn1

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-YPET-optoNES.V416I-WPRE

Plasmid

#159973

Purpose

optical regulation of nuclear export with extended relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.V416I

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

V416I

Promoter

hSyn1

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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AAV-Synapsin-NLS-YPET-optoNES.I427V-WPRE

Plasmid

#159974

Purpose

optical regulation of nuclear export with shorter relaxation time

Depositor

Michael Courtney

Article

Li et al Nat Commun. 2020 Oct 9;11(1):5107. doi:

Insert

NLS-YPET-optoNES.I427V

Use

Adenoviral

Tags

Expression

Mammalian

Mutation

I427V

Promoter

hSyn1

Available since

Oct. 22, 2020

Availability

Academic Institutions and Nonprofits only

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pET-45b-TBP(38Q)-IDR-mCherry

Plasmid

#145283

Purpose

Bacterial Expression of 6xHis TBP(38Q) IDR mCherry Fusion Protein

Depositor

Denes Hnisz

Article

Basu et al Cell. 2020 May 28;181(5):1062-1079.e30.

Insert

TBP(38Q)-IDR (TBP Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Sept. 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a-ybbr-XMod-Doc (WT, S199AzF)-HIS

Plasmid

#153443

Purpose

E. coli expression (amber suppression) of Rc. XDocB with serine at position 199 replaced with amber codon.

Depositor

Michael Nash

Article

Liu et al Nat Commun. 2020 Aug 28;11(1):4321. doi:

Insert

Rc.XDocB

Use

Tags

6xHis and ybbr tag

Expression

Bacterial

Mutation

Serine 199 was mutated to amber codon (TAG)

Promoter

T7 promoter

Available since

July 23, 2020

Availability

Academic Institutions and Nonprofits only

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pET28a human Tankyrase2 full length Y920E

Plasmid

#132639

Purpose

expresses human Tankyrase2 full length Y920E in E. coli

Depositor

Wenqing Xu

Article

DaRosa et al Protein Sci. 2016 Sep;25(9):1744-52. doi

Insert

Tankyrase2 Y920E

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pET11a asyn Q79N Q109N (TAC-->TAT)

Plasmid

#105738

Purpose

Bacterial expression of mutant human alpha synuclein

Depositor

Hilal Lashuel

Insert

alpha-synuclein Q79N Q109N (TAC-->TAT) (SNCA Human)

Use

Tags

Expression

Bacterial

Mutation

Q79N/Q109N

Promoter

T7

Available since

March 29, 2018

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)-Hcp1 K321D

Plasmid

#89288

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100, mutated Lys-321 to Asp

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)-Hcp1 R325D

Plasmid

#89289

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100, mutated Arg-325 to Asp

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)-Hcp1 E322A

Plasmid

#87748

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100, mutated Glu-322 to Ala

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)-Hcp1 R325A

Plasmid

#87749

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100, mutated Arg-325 to Ala

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pET151-Vps4(101-437)-Hcp1 K321A

Plasmid

#87747

Purpose

Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

Depositor

Chris Hill

Article

Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

Insert

Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)

Use

Tags

6xHis-V5-TEV and Hcp1

Expression

Bacterial

Mutation

deleted amino acids 1-100, mutated Lys-321 to Ala

Promoter

T7

Available since

April 10, 2017

Availability

Academic Institutions and Nonprofits only

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pETDUET1-hUCH37 (ISF1) - hNFRKB (1-156) GSGS

Plasmid

#61940

Purpose

Co-expression of hUCH37 (ISF1) and hNFRKB (1-156) GSGS in E.coli

Depositor

Chris Hill

Article

VanderLinden et al Mol Cell. 2015 Feb 17. pii: S1097-2765(1

Inserts

hUCH37 (ISF1) (UCHL5 Human)
hNFRKB (1-156) GSGS (NFRKB Human)

Use

Tags

6x HIS

Expression

Bacterial

Mutation

Construct ends at residue 156, residues 97-100 (N…

Promoter

T7

Available since

Oct. 9, 2015

Availability

Academic Institutions and Nonprofits only
pET-22HT-MSI1(7-192)R53E/R61E

Plasmid

#60354

Purpose

R53E R61E double mutant of the mouse MSI1 RBD

Depositor

Sean Ryder

Article

Clingman et al Elife. 2014 Jun 16:e02848. doi: 10.7554/

Insert

mouse MSI1 (7-192) (Msi1 Mouse)

Use

Tags

His and TEV protease site

Expression

Bacterial

Mutation

R53E and R61E mutations; contains amino acids 7-1…

Promoter

T7

Available since

Nov. 6, 2014

Availability

Academic Institutions and Nonprofits only
pET-21a-GFP-Patronin CC 534–868

Plasmid

#59054

Purpose

for E. coli expression of Patronin CC truncation

Depositor

Ron Vale

Article

Hendershott et al Proc Natl Acad Sci U S A. 2014 Apr 22;11

Insert

Patronin (Patronin Fly)

Use

Tags

GFP

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 27, 2014

Availability

Academic Institutions and Nonprofits only

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HOXD1 (murine, partial) HIS-tag pET

Plasmid

#8560

Depositor

Corey Largman

Insert

HOXD1 (Hoxd1 Mouse)

Use

Tags

His and T7

Expression

Bacterial

Mutation

Partial protein containing 10 AA N-terminal to HD…

Promoter

Available since

May 30, 2006

Availability

Academic Institutions and Nonprofits only
Pet28a His6-HSPB7 (zebrafish) C49S/C117S

Plasmid

#110073

Purpose

Express C49S/C117S double mutant of Zebrafish His6-HSPB7 in bacteria

Depositor

Yimon Aye

Article

Surya et al ACS Chem Biol. 2018 Feb 8. doi: 10.1021/

Insert

His6-HSPB7 (hspb7 Zebrafish)

Use

Tags

His6

Expression

Bacterial

Mutation

Cysteine 49 to Serine, Cysteine 117 to Serine

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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Kv7.5 AB (361-545, Δ395-511)/pET28HMT

Plasmid

#111267

Purpose

Protein expression in E.Coli

Depositor

Dan Minor

Article

Chang et al Neuron. 2018 Feb 21;97(4):836-852.e6. do

Insert

potassium voltage-gated channel subfamily KQT member 5 isoform 1 [Homo sapiens] (KCNQ5 Human)

Use

Tags

His Tag and MBP Tag

Expression

Bacterial

Mutation

Kv7.5 AB (361-545, Δ395-511)

Promoter

T7

Available since

Availability

Academic Institutions and Nonprofits only

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pET His6 GST TEV LIC cloning vector (1G)

Plasmid

#29655

Depositor

Scott Gradia

Type

Empty backbone

Use

Tags

His6-GST-TEV

Expression

Bacterial

Mutation

Promoter

Available since

June 7, 2011

Availability

Academic Institutions and Nonprofits only

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