-
Plasmid#83538PurposeUsed to evaluate the expression output of RPL8B promoter with yEGFP used as the reporter gene.DepositorInsertRPL8B promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL8BAvailable sinceDec. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH3
Plasmid#83553PurposeUsed to evaluate the expression output of HXT7 promoter with yEGFP used as the reporter gene.DepositorInsertHXT7 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterHXT7Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFPH0
Plasmid#83552PurposeUsed to evaluate the expression output of TPS1 promoter with yEGFP used as the reporter gene.DepositorInsertTPS1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTPS1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP90
Plasmid#83547PurposeUsed to evaluate the expression output of TEF2 promoter with yEGFP used as the reporter gene.DepositorInsertTEF2 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterTEF2Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP82
Plasmid#83541PurposeUsed to evaluate the expression output of YEF3 promoter with yEGFP used as the reporter gene.DepositorInsertYEF3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterYEF3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP80
Plasmid#83540PurposeUsed to evaluate the expression output of RPL3promoter with yEGFP used as the reporter gene.DepositorInsertRPL3 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterRPL3Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pILGFP78
Plasmid#83539PurposeUsed to evaluate the expression output of SSB1 promoter with yEGFP used as the reporter gene.DepositorInsertSSB1 promoter-yEGFP
UseTagsExpressionYeastMutationPromoterSSB1Available sinceOct. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas59
Plasmid#82396PurposesgRNA targeting YFP +787 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas57
Plasmid#82395PurposesgRNA targeting YFP +111 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas56
Plasmid#82394PurposesgRNA targeting YFP +46 from TSS; template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas55
Plasmid#82393PurposesgRNA targeting YFP +172 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCas52
Plasmid#82392PurposesgRNA targeting YFP +52 from TSS; non-template strand; expressed from aTc inducible system in pEZ03 in the NS1 locus with kanamycin resistanceDepositorInsertgRNA
UseTagsExpressionMutationPromoterPEZ3Available sinceSept. 15, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK347
Plasmid#71833PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59D), and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationchanges histidine-59 to aspartatePromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
pJK348
Plasmid#71832PurposeCloned region contains Acetobacter aceti 1023 hypothetical protein AZ09_02685, purE(H59N), and purK genesDepositorInsertshypothetical protein
N5-carboxyaminoimidazole ribonucleotide mutase
N5-carboxyaminoimidazole ribonucleotide synthetase
UseTagsExpressionBacterialMutationchanges histidine-59 to asparaginePromoterAvailable sinceJan. 6, 2016AvailabilityAcademic Institutions and Nonprofits only -
23SrRNA.RAV12(wt).2508-2580.GAAAC.duplexH90
Plasmid#26192DepositorInsert23S ribosomal RNA fragment (modified) with duplex H90
UseTagsExpressionBacterialMutationfragment 2508-2580 of E. coli rRNA, with nucleoti…PromoterAvailable sinceDec. 8, 2010AvailabilityAcademic Institutions and Nonprofits only -
23SrRNA.RAV12(wt).2508-2580.GAAAC.G2553U
Plasmid#26191DepositorInsert23S ribosomal RNA fragment (modified) with G2553U mutation
UseTagsExpressionBacterialMutationfragment 2508-2580 of E. coli rRNA, with nucleoti…PromoterAvailable sinceOct. 28, 2010AvailabilityAcademic Institutions and Nonprofits only -
qTAG-N-Solo-mScarlet-TUBA1B
Plasmid#207764PurposeDonor template for mScarlet insertion into the N-terminus of the TUBA1B locus for tubulin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TUBA1B Addgene #207763DepositorArticleInsertTUBA1B Homology Arms flanking a mScarlet Tag (TUBA1B Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pREMI-ZA
Plasmid#183736PurposePlasmid for a restriction enzyme mediated integration (REMI) in Komagataella phaffiiDepositorInsertSh ble
UseRandom insertional gene deletion in komagataella …TagsExpressionMutationPromoterTEF1 promoterAvailable sinceMarch 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl_NHEJ
Plasmid#91249PurposeCRISPRi vector for Yarrowia lipolytica, repressing KU70 and KU80 for enhanced HRDepositorInsertsCodon optimized dCas9-Mxi1
KU70 sgRNA expression cassette
KU80a sgRNA expression cassette
KU80b sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceAug. 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
pCRISPRi_Mxi1_yl
Plasmid#91248PurposeCRISPR-dCas9-Mxi1 vector for Yarrowia lipolytica, expressing dCas9-Mxi1 and AvrII site for sgRNA insertionDepositorInsertsCodon optimized dCas9-Mxi1
sgRNA expression cassette
UseCRISPR and Synthetic BiologyTagsExpressionYeastMutationPromoterSCR1'-tRNA and UAS1B8-TEF(136)Available sinceAug. 25, 2017AvailabilityAcademic Institutions and Nonprofits only