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Plasmid#220630PurposeFor expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceOct. 9, 2024AvailabilityAcademic Institutions and Nonprofits only
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P4-TadCBEd
Plasmid#225143PurposePackaging plasmid for TadCBEd in v3b PE-eVLP architectureDepositorInsertP4-TadCBEd
UseTagsExpressionMammalianMutationPromoterAvailable sinceSept. 17, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIA036
Plasmid#200425PurposeamyE::(Ppen-lacIΔ11-bsdronPA cm). PALM integration vector includes codon optimized dronPA fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorArticleInsertLacI
UseTagsbsdronPAExpressionBacterialMutationPromoterPpenAvailable sinceAug. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
Bneu-10
Plasmid#205215PurposeUnit Bactoneuron-10, expresses E2-crimsonDepositorInsertsE2-Crimson
LuxR
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pIA030
Plasmid#200424PurposethrC::(Ppen-lacIΔ11-PAmCherry1 erm2). PALM integration vector includes PAmCherry1 fusion tagged C-terminal of LacI for FROS imaging in B. subtilis.DepositorArticleInsertLacI
UseTagsPAmCherryExpressionBacterialMutationPromoterPpenAvailable sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA1.0
Plasmid#208297PurposeExpresses ABE-RA1.0 in mammalian cellsDepositorInsertTadA-RA1.0-SpCas9 D10A
UseTagsExpressionMammalianMutationD108GPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
ABE-RA1.1
Plasmid#208298PurposeExpresses ABE-RA1.1 in mammalian cellsDepositorInsertTadA-RA1.1-SpCas9 D10A
UseTagsExpressionMammalianMutationD108G, K161NPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-R334D-TSF
Plasmid#210841PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationR334DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-R326D-TSF
Plasmid#210840PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationR326DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-V314D-TSF
Plasmid#210839PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationV314DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-V44D-TSF
Plasmid#210843PurposeMammalian expression of FIP200 NTD mutantDepositorInsertFIP200 (RB1CC1 Human)
UseTagsExpressionMammalianMutationV44DPromoterAvailable sinceDec. 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_F31YDHFR_R127ATS
Plasmid#191312PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationF31Y DHFR/R127A TYMSPromoterT7 (folA), Tet (thyA)Available sinceOct. 19, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pJS237
Plasmid#200713PurposeADH2 terminator from yeast (MoClo 3´U +Ter)DepositorInsertADH2 Terminator
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-TSF
Plasmid#203545PurposeFIP200 NTD-TwinStrep-Flag mammalian expressionDepositorInsertFIP200 (RB1CC1 Human)
UseTagsTwinStrep FlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-GST
Plasmid#203544PurposeFIP200 NTD-MBP mammalian expressionDepositorInsertFIP200 (RB1CC1 Human)
UseTagsGSTExpressionMammalianMutationPromoterCMVAvailable sinceJuly 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200(1-640)-MBP
Plasmid#203543PurposeFIP200 NTD-GFP mammalian expressionDepositorInsertFIP200 (RB1CC1 Human)
UseTagsMBP-10HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAG-FIP200 (1-640)-msfGFP
Plasmid#203546PurposeFIP200 NTD-GFP-10His mammalian expressionDepositorInsertFIP200 (RB1CC1 Human)
UseTagsmsfGFP-10HisExpressionMammalianMutationPromoterCMVAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_D27NDHFR_Q33STS
Plasmid#191289PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationD27N DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 13, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTETDuet_M42FG121VDHFR_Q33STS
Plasmid#191292PurposeBacterial co-expression of E. coli folA (Dihydrofolate Reductase) and E. coli thyA (Thymidylate Synthase)DepositorInsertfolA, thyA
UseTagsExpressionBacterialMutationM42F/G121V DHFR, Q33S TYMSPromoterT7 (folA), Tet (thyA)Available sinceJune 7, 2023AvailabilityAcademic Institutions and Nonprofits only