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Plasmid#112144PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with Cysteine 258 mutated to selenocysteine (U) and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
UseTagsExpressionMammalianMutationCysteine 258 mutated to selenocysteine (U)PromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pMX-SHP1C-ires-hCD8
Plasmid#183720PurposeExpresses the myc-tagged catalytic domains of wild-type SHP-1.DepositorInsertSHP-1
UseRetroviralTagsExpressionMutationcatalitic domain alonePromoterAvailable sinceJuly 19, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-FLAG-CCR5
Plasmid#98949PurposeMammalian expression plasmid for N-terminal FLAG-tagged human CCR5DepositorInsertCCR5 (CCR5 Human)
UseTagsFLAG (dykdddd) epitope tag and Signal/leader sequ…ExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2816 pPB: CAG-PYL1-VPR-p2A-GID1-ABI-WPRE PGK-GAI-tagBFP-SpdCas9
Plasmid#84261PurposeExpresses AND gate ABA/GA-inducible VPR-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
PYL1-VPR-p2a-GID1-ABI
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, GAI, GID1, HA tag, PYL1, p2a, …ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pGEX-6p-hDcp2 E147/8Q
Plasmid#72217Purposebacterial expression of GST-tagged human Dcp2 Q147/8 mutant (disrupts the decapping function of Dcp2)DepositorInsertDcp2 (DCP2 Human)
UseTagsGSTExpressionBacterialMutationE147Q, E148Q and F404L (please see depositor'…PromotertacAvailable sinceDec. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mCerulean-PA-Rac1
Plasmid#22030PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseTags6X His and mCeruleanExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mVenus-PA-Rac1-T17N
Plasmid#22017PurposeExpression of photoactivatable Rac1 (dominant-negative mutant)DepositorInsertPA-Rac1-T17N (RAC1 Avena sativa (oat), Human)
UseTags6X His and mVenusExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutation T17N.PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
LUC05 (L/Cys/P)
Plasmid#112146PurposeMammalian expression vector to test the effect of substituting selenocysteine for Cys on luciferase enzyme activityDepositorInsertLuciferase coding region with wild type Cysteine 258 and wild-type PHGPx SECIS in the 3' UTR (Gpx4 Photinus pyralis, Rat)
UseTagsExpressionMammalianMutationnonePromoterAvailable sinceJuly 6, 2018AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1K908E-VSV
Plasmid#139357PurposeExpression of kinase-dead JAK1-K908E proteinDepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
UseTagsVSVExpressionMammalianMutationK908EPromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450M
Plasmid#22034DepositorInsertmVenus-PA-Rac1-C450M (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsExpressionMammalianMutationThe LOV domain contains the mutation C450M.Rac1 s…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP-LpIA(wild-type)
Plasmid#61821PurposeEncodes a wild-type sequence of LplA and serves as the negative control for resorufin labelingDepositorInsertE. coli lipoic acid ligase
UseTags6XHis, EGFP, and FlagExpressionMammalianMutationPromoterCMVAvailable sinceAug. 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBabe-TetCMV-puro-mVenus-PA-Rac1-C450A
Plasmid#22023DepositorInsertmVenus-PA-Rac1-C450A (RAC1 Avena sativa (oat), Human)
UseRetroviralTagsExpressionMammalianMutationThe LOV domain contains the mutation C450A.Rac1 s…PromoterAvailable sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pScalps_puro_mIL-2Rβ
Plasmid#59916PurposeExpresses mouse interleukin-2 receptor Beta chainDepositorInsertinterleukin 2 receptor beta (Il2rb Mouse)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceOct. 3, 2014AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-JAK1P1084A-VSV
Plasmid#139358PurposeExpression of the catalytically impaired JAK1-P1084A mutant (corresponding to TYK2-P1104A)DepositorInsertJAK1 cDNA with 300nt of 5' UTR (JAK1 Human)
UseTagsVSVExpressionMammalianMutationP1084APromoterCMVAvailable sinceApril 23, 2020AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2821 pPB: CAG-GID1-VPR-IRES-Puro-WPRE PGK-GAI-tagBFP-SpdCas9-ABI
Plasmid#84257PurposeExpresses Sp dCas9 and GA-inducible VPR for OR gate/diametric regulationDepositorInsertsGAI-tagBFP-Sp dCas9-ABI
GID1-VPR
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), ABI, GAI, GID1, HA tag, IRES-Puro, …ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceFeb. 22, 2018AvailabilityAcademic Institutions and Nonprofits only -
pL90-Nat-2xHO
Plasmid#139069PurposeCRISPR/Cas9 engineering of the HO endonuclease gene with nourseothricin selectionDepositorInsertTwo guide RNAs targeting the HO endonuclease
UseTagsExpressionBacterial and YeastMutationPromoterTDH3Available sinceMay 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pTriEx-mPA-GFP-PA-Rac1
Plasmid#22022PurposeExpression of photoactivatable Rac1DepositorInsertPA-Rac1 (RAC1 Avena sativa (oat), Human)
UseTags6X His and mPA-GFPExpressionBacterial, Insect, and Mamm…MutationRac1 starts at I4 and contains mutations Q61L, E9…PromoterCMV, p10Available sinceSept. 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2813 pPB: CAG-GID1-KRAB-IRES-Puro-WPRE PGK-ABI-tagBFP-SpdCas9
Plasmid#84242PurposeExpresses GA-inducible KRAB-Sp dCas9DepositorInsertsGAI-tagBFP-Sp dCas9
GID1-KRAB
UseCRISPR and Synthetic Biology; PiggybacTags2xNLS (SV40), GAI, GID1, HA tag, IRES-Puro, and t…ExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-FLAG-LplA(AAG)
Plasmid#61307PurposeContains FLAG epitope for immunofluorescence detection of resorufin ligase for mammalian cell expressionDepositorInsertE. coli lipoic acid ligase
UseTags6xHis, Flag, T7 tag, and Xpress epitopeExpressionMammalianMutationE20A, F147A, H149GPromoterCMVAvailable sinceMarch 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
SIGLE-bio-His
Plasmid#53337PurposeExpresses full-length Sialic acid-binding Ig-like lectin 12 precursor ectodomain in mammalian cells. C-terminal rat Cd4d3+4 tag, biotinylation sequence and His tag.DepositorInsertSIGLE (SIGLEC12 Human)
UseTagsHis tag, enzymatic biotinylation sequence, and ra…ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 27, 2015AvailabilityAcademic Institutions and Nonprofits only