Addgene

We narrowed to 12,309 results for: EGFP

Showing: 3721 - 3740 of 12309 results

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pcDNA5-hygro-eGFP-HEC1-9D

Plasmid

#114029

Purpose

Inducible expression of eGFP-tagged HEC1-9D in FLPin Trex cell lines

Depositor

Geert Kops

Article

Etemad et al Nat Commun. 2015 Dec 1;6:8987. doi: 10.1

Insert

eGFP-HEC1 - 9D (NDC80) (NDC80 Human)

Use

Tags

Expression

Mammalian

Mutation

Hec1 - 9D

Promoter

Available since

Sept. 6, 2018

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta-DC-mutant-G435R

Plasmid

#64684

Purpose

expresses single mutated WRAP53beta at G435R, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at G435R

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-R398W

Plasmid

#64683

Purpose

expresses single mutated WRAP53beta at R398W, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at R398W

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
EB3-eGFP-2xC2-iLID

Plasmid

#114419

Purpose

anchor iLID domain to plasma membrane with slower diffusion

Depositor

Dorus Gadella

Article

Van Geel et al Small GTPases. 2018 Sep 5:1-10. doi: 10.

Insert

iLID domain

Use

Tags

eGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 12, 2018

Availability

Academic Institutions and Nonprofits only

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pTox-EGFPsite1_NGA-PAM (BPK754)

Plasmid

#181747

Purpose

p11-lacY-wtx1 derivative toxic plasmid encoding an SpCas9 target site for EGFP site 1 with an NGA PAM

Depositor

Benjamin Kleinstiver

Article

Christie et al Nat Biotechnol. 2022 Oct 6. pii: 10.1038

Insert

ccdB toxin gene and SpCas9 target site (EGFP site 1 NGA PAM)

Use

Selection plasmid

Tags

Expression

Mutation

Promoter

Available since

March 31, 2022

Availability

Academic Institutions and Nonprofits only

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pCAG-AaCas12b(R911A)-2AeGFP

Plasmid

#121961

Purpose

Mammalian expression, Transcription regulation, deactivated AaCas12b

Depositor

Wei Li

Article

Teng et al Cell Discov. 2018 Nov 27;4:63. doi: 10.1

Insert

Catalytically deactivated AaCas12b

Use

Tags

3xHA tag

Expression

Mammalian

Mutation

R911A

Promoter

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pMXs-IP-EGFP-ULK1(1-828)

Plasmid

#38198

Depositor

Noboru Mizushima

Article

Hara et al J Cell Biol. 2008 May 5. 181(3):497-510.

Insert

a novel protein kinase structurally related to C. elegans UNC-51 (Ulk1 Mouse)

Use

Retroviral

Tags

EGFP

Expression

Mammalian

Mutation

deleted amino acids 829-1051

Promoter

Available since

Sept. 24, 2012

Availability

Academic Institutions and Nonprofits only
pENTR/pTER shEGFP #1 (628-2)

Plasmid

#17470

Purpose

Gateway entry vector for EGFPshRNA#1 under H1/TO promoter

Depositor

Eric Campeau, Paul Kaufman

Article

Campeau et al PLoS One. 2009 Aug 6;4(8):e6529.

Insert

Enhanced green fluorescent protein shRNA #1

Use

RNAi; Entry vector

Tags

Expression

Mutation

Promoter

Available since

Aug. 12, 2009

Availability

Academic Institutions and Nonprofits only

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pBabe bleo IRES-EGFP

Plasmid

#14432

Depositor

L. Miguel Martins

Insert

IRES-EGFP

Use

Retroviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 16, 2007

Availability

Academic Institutions and Nonprofits only

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pci-EGFP-UPF1-DEAA

Plasmid

#135996

Purpose

DEAA UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Use

Tags

EGFP

Expression

Mammalian

Mutation

DE647AA

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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PasCas13b-gRNA-EGFP(W58stop)

Plasmid

#180507

Purpose

PasCas13b-gRNA targeting EGFP(W58stop) for RNA editing

Depositor

Masaharu Somiya

Article

Somiya et al bioRxiv 2022.01.25.477620

Insert

PasCas13b-gRNA targeting EGFP(W58stop)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

March 17, 2022

Availability

Academic Institutions and Nonprofits only

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pci-EGFP-UPF1-R615A

Plasmid

#135995

Purpose

R615A UPF1 inserted with GFP tagged on the N-terminus used to stably integrate into cells

Depositor

Anthony Leung

Article

Fischer et al Mol Cell. 2020 Apr 2;78(1):70-84.e6. doi

Insert

UPF1

Use

Tags

EGFP

Expression

Mammalian

Mutation

R615A

Promoter

Available since

Feb. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pEGFP-WRAP53beta-DC-mutant-F164L

Plasmid

#64681

Purpose

expresses single mutated WRAP53beta at F164L, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at F164L

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
pEGFP-WRAP53beta-DC-mutant-H376Y

Plasmid

#64682

Purpose

expresses single mutated WRAP53beta at H376Y, found in Dyskeratosis congenita patients

Depositor

Marianne Farnebo

Article

Henriksson et al Genes Dev. 2014 Dec 15;28(24):2726-38. d

Insert

WD40-encoding RNA antisense to P53 (WRAP53 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

mutation at H376Y

Promoter

CMV

Available since

May 21, 2015

Availability

Academic Institutions and Nonprofits only
GFP-Clavesin1 (pEGFP-C1-Sec14a)

Plasmid

#47421

Depositor

Peter McPherson

Article

Katoh et al J Biol Chem. 2009 Oct 2;284(40):27646-54

Insert

Clavesin1 (CLVS1 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2014

Availability

Academic Institutions and Nonprofits only
pEGFP-RNF138-delta 86-105

Plasmid

#78922

Purpose

Mammalian expression of RNF138 with delta 86-105 and an EGFP fusion

Depositor

Michael Hendzel

Article

Ismail et al Nat Cell Biol. 2015 Nov;17(11):1446-57.

Insert

RNF138 (RNF138 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

delta 86-105

Promoter

CMV

Available since

June 27, 2016

Availability

Academic Institutions and Nonprofits only
pEGFP-YOD1-MutS2-C160S

Plasmid

#85668

Purpose

Expression of human YOD1 I292Q V295Q C160S mutant with N-terminal GFP tag

Depositor

Hemmo Meyer

Article

Papadopoulos et al EMBO J. 2016 Oct 17. pii: e201695148.

Insert

YOD1 (YOD1 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

C160S and I292Q and V295Q

Promoter

CMV

Available since

April 28, 2017

Availability

Academic Institutions and Nonprofits only

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pDRF1-GW yoeGFP-T2A-mCherry

Plasmid

#118446

Purpose

Produces equimolar expression of yeast codon-optimized eGFP and mCherry

Depositor

Bas Teusink

Article

Botman et al Sci Rep. 2019 Feb 19;9(1):2234. doi: 10.

Insert

yoeGFP-T2A-mCherry

Use

Tags

Expression

Yeast

Mutation

Promoter

Available since

June 3, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-RIα(D/D+linker)-EGFP

Plasmid

#181842

Purpose

EGFP-tagged docking/dimerization (D/D) and linker region of PKA RIα; for mammalian expression.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

RIα-(D/D+linker)EGFP (PRKAR1A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Contains only residues 12-112 of PKA RIα.

Promoter

CMV

Available since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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Nano-lantern-Actin/pEGFP-C1

Plasmid

#51972

Purpose

mammalian expression of Nano-lantern, a luminescent protein for high-speed single-cell and whole body imaging, fused to actin

Depositor

Takeharu Nagai

Article

Saito et al Nat Commun. 2012;3:1262. doi: 10.1038/nc

Insert

Nano-lantern

Use

Tags

Actin

Expression

Mammalian

Mutation

S257G in Rluc8

Promoter

Available since

March 24, 2014

Availability

Academic Institutions and Nonprofits only