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Plasmid Kit#1000000240PurposeThe YSD Toolkit is a collection of 34 plasmids that can be used in conjunction with the MoClo Yeast Toolkit (YTK) to optimize secretion and cell surface display of proteins of interest in yeast.DepositorAvailable sinceApril 10, 2024AvailabilityAcademic Institutions and Nonprofits only
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pAAV-GFAP-hM3D(Gq)-mCherry
Plasmid#50478PurposeGq-coupled hM3D DREADD fused with mCherry under the control of GFAP promoterDepositorHas ServiceAAV5InserthM3D(Gq)-mCherry
UseAAVTagsmCherryExpressionMutationSee supplemental documents for DREADD mutationsPromoterGFAPAvailable sinceApril 17, 2014AvailabilityAcademic Institutions and Nonprofits only -
MCS-13X Linker-BioID2-HA
Plasmid#80899PurposeTo fuse your protein of interest to the N-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a flexible 25nm GS linker are present upstream of BioID2 and HA tags.DepositorHas ServiceCloning Grade DNATypeEmpty backboneUseTagsHA and humanized A. aeolicus BioID2 with R40GExpressionMammalianMutationPromoterCMVAvailable sinceOct. 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pSBbi-RP
Plasmid#60513PurposeSB-transposon with constitutive bi-directional promoter, one side: SfiI cloning site for GOI (contains filler DNA), other side: RFP and puromycin resistance geneDepositorTypeEmpty backboneUseTransposonTagsExpressionMammalianMutationPromoterEF1a/RPBSAAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
Halo-TOMM20-N-10
Plasmid#123284PurposeLocalization: Outer Mitochondrial MembraneDepositorInsertTOMM20 (TOMM20 Human)
UseTagsHaloTag7ExpressionMammalianMutationPromoterCMVAvailable sinceApril 24, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET28a-MSP1D1delltaH5
Plasmid#71714PurposeMembrane scaffold protein (MSP), helix 5 deletionDepositorInsertApolipoprotein A1 (APOA1 Human)
UseTags6xHis and TEV siteExpressionBacterialMutationPromoterAvailable sinceDec. 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mRNA-CleanCap-NanoLuc
Plasmid#188247PurposeTemplate plasmid to transcribe mRNA encoding NanoLucDepositorInsertNanoLuc
UseLuciferaseTagsExpressionMammalianMutationPromoterT7Available sinceSept. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS185 CMV-Cre
Plasmid#11916PurposeMammalian expression of Cre recombinase driven by the human cytomegalovirus IE promoterDepositorInsertcre (cre bacteriophage P1)
UseCre/LoxTagsExpressionMammalianMutationPromoterAvailable sinceJan. 4, 2007AvailabilityAcademic Institutions and Nonprofits only -
pHAGE hSPC-eGFP-W
Plasmid#36450DepositorInsertsUseLentiviralTagsExpressionMutationPromoterN/A and human SP-C promoterAvailable sinceMay 11, 2012AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-miRFP670nano3
Plasmid#184664PurposeMonomeric near-infrared fluorescent protein miRFP670nano3 in mammalian plasmidDepositorInsertmiRFP670nano3
UseTagsHisExpressionMammalianMutationPromoterCMVAvailable sinceJune 23, 2022AvailabilityAcademic Institutions and Nonprofits only -
pHR_Gal4UAS_pGK_mCherry
Plasmid#79124PurposeLentiviral vector for inducible expression of genes with Gal4DBDVP64 synNotch Receptors (no IRES)DepositorTypeEmpty backboneUseLentiviralTagsExpressionMutationPromoterAvailable sinceJuly 26, 2016AvailabilityAcademic Institutions and Nonprofits only -
ER-mRFP
Plasmid#62236PurposePlasmid expresses endoplasmic reticulum targeted mRFP in mammalian cells.DepositorInsertER-mRFP
UseTagsmRFP1ExpressionMammalianMutationPromotercmvAvailable sinceMarch 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
N174-MCS
Plasmid#81061PurposeLentiviral vector for constitutive gene expression.DepositorTypeEmpty backboneUseLentiviralTagsExpressionMammalianMutationPromoterEF-1αAvailable sinceSept. 9, 2016AvailabilityAcademic Institutions and Nonprofits only -
pAAV-mTH-GFP
Plasmid#99128PurposeAn AAV genome that expresses GFP from the mouse TH promoterDepositorInsertGFP
UseAAVTagsExpressionMammalianMutationPromotermTHAvailable sinceAug. 21, 2017AvailabilityAcademic Institutions and Nonprofits only -
pSB700
Plasmid#64046PurposeLentiviral vector for expressing U6 sgRNA and CAGGS Cerulean fluorescent proteinDepositorTypeEmpty backboneUseCRISPR, Lentiviral, and Synthetic BiologyTagsExpressionMammalianMutationPromoterU6, CAGGSAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
mEmerald-FilaminA-N-9
Plasmid#54098PurposeLocalization: Actin/Focal Adhesions, Excitation: 487, Emission: 509DepositorInsertFilaminA (FLNA Human)
UseTagsmEmeraldExpressionMammalianMutationD2626HPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pAC-GAMMAipi
Plasmid#53278PurposeHas ctrE, crtB, crtI, crtY, and idi genes of Erwinia herbicola (Pantoea agglomerans) Eho10 and crtYm gene of marine bacterium P99-3. and produces gamma-carotene in Escherichia coliDepositorInsertcrtYm
UseLow copy number bacterial cloning vectorTagsExpressionMutationPromoterendogenous promoterAvailable sinceJan. 21, 2015AvailabilityAcademic Institutions and Nonprofits only -
RA-Sec61β
Plasmid#153978PurposeDimerization dependent fluorescent protein RA domain fused to ER protein Sec61βDepositorInsertSec61 Transolocon Subunit Beta (SEC61B Human)
UseTagspAcGFP-C1ExpressionMammalianMutationPromoterAvailable sinceJuly 21, 2020AvailabilityAcademic Institutions and Nonprofits only -
Anti-Cav3.2 Ca2+ channel [N55/10R]
Plasmid#177562PurposeMammalian Expression Plasmid of anti-Cav3.2 Ca2+ channel (Human). Derived from hybridoma N55/10.DepositorInsertanti-Cav3.2 Ca2+ channel (Homo sapiens) recombinant mouse monoclonal antibody (CACNA1H Mouse)
UseTagsExpressionMammalianMutationPromoterDual CMVAvailable sinceNov. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
pZW1-FCS-circRNA
Plasmid#73449PurposeExpression of circular RNADepositorInsertPOLR2A (POLR2A Human)
UseTagsnoneExpressionMammalianMutationnonePromoterCMV promoterAvailable sinceFeb. 24, 2016AvailabilityAcademic Institutions and Nonprofits only