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Plasmid#120353PurposepiggyBac transposon for dox-inducible expression of the OKMS (Oct4, Klf4[10-483], c-Myc, Sox2) polycistronic reprogramming cassette (with mCherry). SpCas9 is constitutively expressed.DepositorInsertOKMS cassette
UseCRISPR; Piggybac transposonTagsExpressionMammalianMutationKlf4 [10-483]PromoterAvailable sinceApril 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
(565) pAAV Alb-AAT KRAB-SadCas9 U6-gSTOP
Plasmid#163030PurposeExpression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseAAVTagsKRAB, SV40 pA, and myc NLSExpressionMammalianMutationPromoterAlb-AATAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAFMW-Nbr-D435A/E437A
Plasmid#40829PurposeExpresses Flag, Myc tagged mutant Nbr in Drosophila cellsDepositorInsertNbr D435A/E437A (Nbr Fly)
UseTags3xFLAG and 6xMycExpressionInsectMutationD435A/E437A, part of the DEDD motif; mutations p…PromoterActin5CAvailable sinceSept. 4, 2013AvailabilityAcademic Institutions and Nonprofits only -
Lenti-evoA1-seBEN_empty
Plasmid#174701PurposeSmall-molecule controlled base editingDepositorInsertLenti-evoA1-seBEN
UseLentiviralTagsmyc tagExpressionMutationPromoterEFS/U6Available sinceNov. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
FRB_nPPVp
Plasmid#118966PurposeExpresses N-terminus of plum pox virus protease (PPVp) fused to FKBP-rapamycin binding (FRB) domainDepositorInsertsplit nPPVp in fusion with FRB
UseTagsMyc tagExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 22, 2019AvailabilityAcademic Institutions and Nonprofits only -
pAAV_CaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
Plasmid#163698PurposeAAV vector expressing CaMPARI2_F391W primarily in excitatory neurons in rodent cortexDepositorInsertCaMKII_NES-his-CaMPARI2-F391W-WPRE-SV40
UseAAVTagsFLAG-HA-myc and NES_hisExpressionMammalianMutationPromoterCaMKII fragmentAvailable sinceMarch 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
RalGDS-YPet
Plasmid#134929PurposeFluorescently tagged affinity reagentDepositorInsertRaIGDS RBD, YPet
UseTagsMycExpressionMammalianMutationPromoterAvailable sinceMarch 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
pHAGE2-EF1a-MCS-dOB-hPOT1_IRES-blast
Plasmid#166413Purposeexpress hPOT1 ΔOB in mammalian cellsDepositorInsertProtection Of Telomeres 1 (POT1 Human)
UseLentiviralTagsmycExpressionMammalianMutationΔOB deleted 125 a.aPromoteref1-aAvailable sinceMay 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PIWI(aa478-860)
Plasmid#21543DepositorInsertAgo2 (AGO2 Human)
UseTags3XFLAGExpressionMammalianMutationcontain only aa478-860PromoterAvailable sinceJuly 13, 2009AvailabilityAcademic Institutions and Nonprofits only -
pcDNA-Fah_W
Plasmid#163087Purposeexpressing mouse wild type FAH in mammalian cellsDepositorInsertFah_W
UseTagsMyc-HisExpressionMammalianMutationPromoterCMVAvailable sinceJan. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3 PBD HK-AA (Nigg AH13)
Plasmid#41161DepositorInsertPBD HK-AA (PLK1 Human)
UseTags3xMyc and PreScission SiteExpressionMammalianMutationContains Plk1-PBD only (aa 326–603); HK-AA (H538A…PromoterCMVAvailable sinceJune 7, 2013AvailabilityAcademic Institutions and Nonprofits only -
FLAG-PAZ(aa1-480)
Plasmid#21529DepositorInsertAgo2 (AGO2 Human)
UseTags3XFLAGExpressionMammalianMutationcontain only aa1-480PromoterAvailable sinceJuly 29, 2009AvailabilityAcademic Institutions and Nonprofits only -
pZ_P-GAP-ACS1
Plasmid#126712Purposeyeast plasmid complementing ACS1 from Komagataella phaffiiDepositorInsertacs1
UseTags6xHis,MycExpressionYeastMutationPromoterGAPAvailable sinceJune 19, 2019AvailabilityAcademic Institutions and Nonprofits only -
(517) SB KRAB-SadCas9 U6-gStop
Plasmid#163023PurposeSleeping Beauty TET-On expression of CRISPRi with gSTOP gRNADepositorInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianMutationPromoterTet-OnAvailable sinceJan. 8, 2021AvailabilityAcademic Institutions and Nonprofits only -
pMA3379
Plasmid#46874PurposeA lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3DepositorInsertsoluble guanylate cyclase 1alpha3 (Gucy1a3 Rat)
UseLentiviralTagsMycExpressionMutationPromoterTRE-TightAvailable sinceDec. 11, 2013AvailabilityAcademic Institutions and Nonprofits only -
pCI syn iGluf
Plasmid#106121PurposeFast indicator for synaptic glutamate imaging (synapsin promoter)DepositorInsertiGluSnFR E25D variant
UseTagsMyc and PDGFRExpressionMammalianMutationChanged Glu 25 to AspPromotersynapsinAvailable sinceJune 18, 2018AvailabilityAcademic Institutions and Nonprofits only -
(364) SB KRAB-SadCas9
Plasmid#163022PurposeSleeping Beauty TET-On expression of CRISPRi (without U6-gRNA)DepositorInsertMammalian codon-optimized SadCas9
UseTransposonTagsKRAB and myc NLSExpressionMammalianMutationPromoterTET-OnAvailable sinceJan. 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRS406-PHO5-GFP-CERT PH domain
Plasmid#58725PurposeExpresses GFP-Tagged CERT PH domain in yeastDepositorInsertcollagen, type IV, alpha 3 binding protein PH domain (CERT1 Human)
UseTagsGFP and MycExpressionYeastMutationPromoterPHO5Available sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pFRT-TODestFLAGHA_RBPMS_KDR_HindIII
Plasmid#65662PurposeFLAG-HA expression vector with RBPMS carrying mutation to introduce HindIII site and encoding silent mutation to render resistant to siRNA R2 (Farazi et al. 2014)DepositorInsertRBPMS (RBPMS Human)
UseTagsFLAG HAExpressionMammalianMutationintroduced HindIII site (final goal to exchange F…PromoterCMV, 2x TetAvailable sinceJune 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pMA3431
Plasmid#46876PurposeA lentiviral vector for the doxycycline-inducible expression of soluble guanylate cyclase1 alpha3 mutant R592QDepositorInsertsoluble guanylate cyclase 1alpha 3 (Gucy1a3 Rat)
UseLentiviralTagsMycExpressionMutationR592QPromoterTRE-TightAvailable sinceAug. 13, 2013AvailabilityAcademic Institutions and Nonprofits only