Addgene

We narrowed to 5,043 results for: pcdna3.1

Showing: 3681 - 3700 of 5043 results

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YFP Gamma 10

Plasmid

#36108

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 10 (Gng10 Mouse)

Use

Tags

YFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
Const.3 EGFP-P2A-dTomato-T2A-TagBFP-PS9

Plasmid

#231907

Purpose

For packaging EGFP, dTomato and TagBFP mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Qi et al Nucleic Acids Research, Volume 53, Issue

Insert

EGFP-P2A-dTomato-T2A-TagBFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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Const.5 EGFP-P2A-dTomato-IRES-Luc-PS9

Plasmid

#231909

Purpose

For packaging EGFP, dTomato and firefly luciferase mRNA into SARS-CoV-2 virus-like particles (VLPs)

Depositor

Sriram Neelamegham

Article

Qi et al Nucleic Acids Research, Volume 53, Issue

Insert

EGFP-P2A-dTomato-IRES-Luc

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 4, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-S2-mRuby2-Vt

Plasmid

#233227

Purpose

Vinculin FRET tension sensor with coiled-coil S2 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S2-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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Vh-Clover-CC-M2-mRuby2-Vt

Plasmid

#233231

Purpose

Vinculin FRET tension sensor with coiled-coil M2 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-M2-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vinculin-Clover-CC-Mad-mRuby2

Plasmid

#233239

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-Mad-mRuby2

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-S1-mRuby2-Vt

Plasmid

#233230

Purpose

Vinculin FRET tension sensor with coiled-coil S1 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S1-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-S0-mRuby2-Vt

Plasmid

#233226

Purpose

Vinculin FRET tension sensor with coiled-coil S0 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S0-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-S4-mRuby2-Vt

Plasmid

#233228

Purpose

Vinculin FRET tension sensor with coiled-coil S4 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S4-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-S7-mRuby2-Vt

Plasmid

#233229

Purpose

Vinculin FRET tension sensor with coiled-coil S7 domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-S7-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vh-Clover-CC-Mad-mRuby2-Vt

Plasmid

#233232

Purpose

Vinculin FRET tension sensor with coiled-coil Mad domain

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vh-Clover-CC-Mad-mRuby2-Vt

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Vinculin-Clover-CC-S0-mRuby2

Plasmid

#233233

Purpose

Vinculin FRET tension sensor with coiled-coil S0 domain no vinculin force control

Depositor

Khalid Salaita

Article

Liu et al Angew Chem Int Ed Engl. 2025 Jan 13:e202

Insert

Vinculin-Clover-CC-S0-mRuby2

Use

Tags

Expression

Mammalian

Mutation

Residues 1-228 of Clover, residues 1-3 deleted fr…

Promoter

CMV

Available since

Feb. 27, 2025

Availability

Academic Institutions and Nonprofits only

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mproSAAS (21 kDa)

Plasmid

#231634

Purpose

Mammalian expression of the N-terminal domain of the mouse chaperone protein proSAAS-(1-180)

Depositor

Iris Lindberg

Article

Fortenberry et al J Biol Chem. 2002 Feb 15;277(7):5175-86.

Insert

Pcsk1N (Pcsk1n Mouse)

Use

Tags

Expression

Mammalian

Mutation

Deletion of C-terminus (aa181-225)

Promoter

Available since

Feb. 11, 2025

Availability

Academic Institutions and Nonprofits only

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pTBL435

Plasmid

#226628

Purpose

pcDNA-dTdT

Depositor

Chang Liu

Article

Carlson et al bioRxiv [Preprint]. 2024 Jun 11:2024.06.

Insert

catalytically deactivated TdT (DNTT Human)

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

D343E, D345E

Promoter

CMV

Available since

Oct. 23, 2024

Availability

Academic Institutions and Nonprofits only

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L2383-FRB-GPA-NLuc

Plasmid

#222902

Purpose

Positive control receptor chain for Nanoluciferase signal. FRB ectodomain, human Glycophorin A (GPA) scaffold, 20 GS linker, full Nanoluciferase.

Depositor

Joshua Leonard

Article

Dolberg et al ACS Synth Biol. 2024 Apr 19;13(4):1273-1

Insert

FRB-GPA-20GS-NanoLuc

Use

Tags

Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 12, 2024

Availability

Academic Institutions and Nonprofits only

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Cas9-FCPF

Plasmid

#220141

Purpose

Expresses Cas9-FCPF in mammalian cells

Depositor

Xinlai Cheng

Article

Gama-Brambila et al JACS Au. 2021 May 12;1(6):777-785. doi:

Insert

Cas9-FCPF (cas9 Synthetic, S. Pyogenes)

Use

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(E162A,M164A,R167A)-3xFLAG-pCDNA

Plasmid

#201964

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E162A,M164A,R167A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
TRAPa(ERED198-201AAAA)-3xFLAG-pCDNA

Plasmid

#201965

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

E198A, R199A,E200A, D201A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(RKRKR232-236AAAAA)-3xFLAG-pCDNA

Plasmid

#201967

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

R232A, K233A, R234A, K235A, R236A

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

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TRAPa(W255A,N262E,K266E)-3xFLAG-pCDNA

Plasmid

#201968

Purpose

Mammalian expression of mutated Translocon-associated protein subunit alpha

Depositor

Ville Paavilainen

Article

Karki et al EMBO Rep. 2023 Dec 6;24(12):e57910. doi:

Insert

SSR1 (SSR1 Human)

Use

Tags

3xFLAG-tag

Expression

Mammalian

Mutation

W255A,N262E,K266E

Promoter

Available since

Jan. 4, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge