Addgene

We narrowed to 5,235 results for: hwa

Showing: 3661 - 3680 of 5235 results

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pLNHA-C1-HsUPF3biso2_I

Plasmid

#146568

Purpose

Mammalian Expression of HsUPF3Biso2

Depositor

Elisa Izaurralde

Article

Kashima et al Genes Dev. 2010 Nov 1. 24(21):2440-50.

Insert

HsUPF3Biso2 (UPF3B Human)

Use

Tags

Expression

Mammalian

Mutation

one silent mutation T510C compared to the sequenc…

Promoter

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pRSFDuet-1_VNp-mNeongreen-Erythropoietin

Plasmid

#182423

Purpose

Bacterial expression of Vesicle Nucleating peptide-mNeongreen-Erythropoietin fusion

Depositor

Dan Mulvihill

Article

Eastwood et al Cell Rep Methods. 2023 Feb 2;3(2):100396

Insert

VNp-mNeongreen-eythropoietin (EPO Human)

Use

Tags

Vesicle Nucleating peptide (VNp) & mNeongreen

Expression

Bacterial

Mutation

Promoter

T7

Available since

Feb. 3, 2023

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYap1-4

Plasmid

#193670

Purpose

Tet inducible knockdown of Yap1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

Yap1 (Yap1 Mouse)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYAP1-6

Plasmid

#193667

Purpose

Tet inducible knockdown of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO-Tet-On shYAP1-2

Plasmid

#193665

Purpose

Tet inducible knockdown of YAP1

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 20, 2022

Availability

Academic Institutions and Nonprofits only

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pLKO.1 puro shYAP1-2

Plasmid

#193693

Purpose

Constitutive lentiviral expression of YAP1 shRNA

Depositor

William Hahn

Article

Kim et al Dev Cell. 2022 Jan 24;57(2):212-227.e8.

Insert

YAP1 (YAP1 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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DDX11-1

Plasmid

#155503

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

DDX11 (DDX11 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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CSTF3

Plasmid

#155487

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

CSTF3 (CSTF3 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-coHPDL H163A H258A 3xFLAG-V5

Plasmid

#174131

Purpose

Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Codon optimized, H163A, H258A

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pLenti6.2-coHPDL H258A 3xFlag-V5

Plasmid

#174130

Purpose

Expresses codon-optimized catalytically inactive HPDL with a 3xFlag-V5 tag in mammalian cells

Depositor

Michael Pacold

Article

Banh et al Nature. 2021 Sep 1. pii: 10.1038/s41586-

Insert

4-hydroxyphenylpyruvate dioxygenase like (HPDL Human)

Use

Lentiviral

Tags

3xFLAG-V5

Expression

Mammalian

Mutation

Codon optimized, H258A

Promoter

Available since

Sept. 20, 2021

Availability

Academic Institutions and Nonprofits only

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pInducer10-UBC9-sh1

Plasmid

#168986

Purpose

Expresses RFP cDNA and UBC9 shRNA 1 in mammalian cells

Depositor

Ji Luo

Article

Yu et al Proc Natl Acad Sci U S A. 2015 Apr 7;112

Insert

Ubiquitin Conjugating Enzyme 9 (UBE2I )

Use

Tags

Expression

Mammalian

Mutation

Promoter

Ubc promoter

Available since

June 1, 2021

Availability

Academic Institutions and Nonprofits only

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deltaVP1 + VP2C-MIOX

Plasmid

#166680

Purpose

Yeast integrative plasmid for expressing deltaVP1 (GAL1 promoter) and mouse myo-inositol oxygenase tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-MIOX
Murine polyomavirus deltaVP1 (NLS deletion mutant)

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

GAL1 and GAL10

Available since

April 9, 2021

Availability

Academic Institutions and Nonprofits only

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wtVP1 + VP2C-GFP

Plasmid

#166674

Purpose

Yeast integrative plasmid for expressing wild-type Murine polyomavirus VP1 (GAL1 promoter) and GFP tagged with VP2C (GAL10 promoter). Contains uracil auxotrophic marker (K. lactis URA3).

Depositor

Claudia Vickers

Article

Cheah et al ACS Synth Biol. 2021 Dec 17;10(12):3251-

Inserts

VP2C-GFP
Wild-type Murine polyomavirus VP1

Use

Synthetic Biology

Tags

Expression

Yeast

Mutation

Promoter

GAL1 and GAL10

Available since

April 8, 2021

Availability

Academic Institutions and Nonprofits only

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AdMKP-3 S159AS197A

Plasmid

#131523

Purpose

Expresses MKP-3 with S159A mutation and S197A mutation in mammalian cells

Depositor

Haiyan Xu

Article

Feng et al Mol Cell Endocrinol. 2012 Sep 25;361(1-2

Insert

Mitogen-activated protein kinase phosphatase 3 (Dusp6 Mouse)

Use

Adenoviral

Tags

No

Expression

Mutation

changed Serine 159 to Alanine and Serine 197 to A…

Promoter

CMV

Available since

Oct. 22, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR149

Plasmid

#106049

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR149; Opto-GPR149; E3)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR149 (GPR149 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR153

Plasmid

#106053

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR153; Opto-GPR153; E7)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR153 (GPR153 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR160

Plasmid

#106054

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR160; Opto-GPR160; E8)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR160 (GPR160 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR162

Plasmid

#106056

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR162; Opto-GPR162; E10)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR162 (GPR162 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR171

Plasmid

#106057

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR171; Opto-GPR171; E11)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR171 (GPR171 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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Opto-GPR174

Plasmid

#106059

Purpose

Expression of a chimeric G-protein coupled receptor (Rhodopsin-GPR174; Opto-GPR174; F1)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-GPR174 (GPR174 Human, Bovine)

Use

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge