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Showing: 3641 - 3660 of 4772 results

  2. p28-2iDMD-puro

    Plasmid
    #88895
    Purpose
    28°C full-length DMD luc-puro-red plasmid. Co-expresses human DMD, firefly luciferase, puroR and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites, but *cannot* be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    puromycin resistance enzyme
    mCherry
    Use
    Tags
    Expression
    Mammalian
    Mutation
    codon optimized
    Promoter
    Available since
    Jan. 5, 2018
    Availability
    Academic Institutions and Nonprofits only
    88895-plasmid-map-sequence-id-451851

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  5. pHEE401E

    Plasmid
    #71287
    Purpose
    Egg cell-specific promoter-controlled expression of 3×FLAG-NLS-zCas9-NLS. Contains gRNA scaffold for insertion of target sequence (U6-26 promoter), Hyg resistance
    Depositor
    Qi-Jun Chen
    Article
    Wang et al Genome Biol. 2015 Jul 21;16:144. doi: 10
    Inserts
    gRNA scaffold
    zCas9
    Use
    CRISPR; Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    Zea mays codon-optimized Cas9
    Promoter
    EC1.2 enhancer fused to EC1.1 promoter and U6-26p…
    Available since
    Jan. 14, 2016
    Availability
    Academic Institutions and Nonprofits only
    71287-plasmid-map-sequence-id-352460

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  6. pETM33_Nsp16

    Plasmid
    #156482
    Purpose
    Bacterial expression of Sars-CoV2 Nsp16 protein with His-tag and GST-tag
    Depositor
    Ylva Ivarsson
    Article
    Mihalic et al Nat Commun. 2023 Sep 13;14(1):5636. doi:
    Insert
    Nsp16 (ORF1ab Synthetic, Severe acute respiratory syndrome coronavirus 2)
    Use
    Tags
    His-GST
    Expression
    Bacterial
    Mutation
    Gene insert is codon optimized for Ecoli.
    Promoter
    T7/LacO
    Available since
    Aug. 4, 2020
    Availability
    Academic Institutions and Nonprofits only
    156482-plasmid-map-sequence-id-307736

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  7. lentiCRISPR - EGFP sgRNA 1

    Plasmid
    #51760
    Purpose
    Expresses human codon-optimized Cas9 protein and puromycin resistance from EFS promoter and an EGFP targeting synthetic single-guide RNA (sgRNA) element from U6 promoter. Lentiviral backbone.
    Depositor
    Feng Zhang
    Article
    Shalem et al Science. 2014 Jan 3;343(6166):84-7. doi:
    Inserts
    Cas9
    Puromycin resistance
    EGFP sgRNA 1
    Use
    CRISPR and Lentiviral
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    EFS and U6
    Available since
    March 11, 2014
    Availability
    Academic Institutions and Nonprofits only
    51760-plasmid-map-sequence-id-392672

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  8. pcDNA3/er-(n2)oxStayGold(c4)v2.0

    Plasmid
    #186296
    Purpose
    A new version of er-(n2)oxStayGold(c4), which gives a higher expression level. The StayGold gene has codon optimization for mammalian expression. For brighter ER imaging if permitted.
    Depositor
    Atsushi Miyawaki
    Article
    Hirano et al Nat Biotechnol. 2022 Apr 25. pii: 10.103
    Insert
    er-(n2)oxStayGold(c4)v2.0
    Use
    Tags
    Expression
    Mammalian
    Mutation
    Promoter
    CMV
    Available since
    July 8, 2022
    Availability
    Academic Institutions and Nonprofits only
    186296-plasmid-map-sequence-id-366145

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  9. Lenti-EFS-BirAopt-GSQ-RBXN-P2A-BLAST

    Plasmid
    #208048
    Purpose
    Enables constitutive expression of BirAopt alone; RBXN represents a EcoR1-BsiW1-Xba1-Not1 polylinker; to perform bioE3; selection with blasticidin
    Depositor
    James Sutherland, Rosa Barrio
    Article
    Barroso-Gomila et al Nat Commun. 2023 Nov 23;14(1):7656. doi:
    Insert
    BirAopt
    Use
    Lentiviral
    Tags
    Expression
    Mutation
    BirAopt is human-codon-optimized BirA (PMID 15707…
    Promoter
    EFS
    Available since
    Nov. 29, 2023
    Availability
    Academic Institutions and Nonprofits only
    208048-plasmid-map-sequence-id-410202

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  13. pMXS-mitoGshF-blasticidin

    Plasmid
    #210314
    Purpose
    Expresses mitochondrially targeted, humanized Streptococcus thermophilus GshF in mammalian cells
    Depositor
    Kivanc Birsoy
    Article
    Wang et al Nature. 2021 Nov;599(7883):136-140. doi:
    Insert
    Bifunctional glutamate-cysteine ligase/glutathione synthetase GshF (gshAB Streptococcus thermophilus)
    Use
    Retroviral
    Tags
    FLAG peptide, Homo sapiens ACO2 mitochondrial tar…
    Expression
    Mutation
    Codon optimized for expression in human cells
    Promoter
    Available since
    Jan. 9, 2024
    Availability
    Academic Institutions and Nonprofits only
    210314-plasmid-map-sequence-id-418848

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Showing: 3641 - 3660 of 4772 results