Addgene

We narrowed to 6,744 results for: chm

Showing: 3641 - 3660 of 6744 results

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pET22b-mEB2-eGFP-6xHis

Plasmid

#130982

Purpose

Bacterial expression of mouse EB2-GFP-6xHis for protein purification.

Depositor

Anne Straube

Article

Roth et al J Cell Sci. 2018 Oct 31;132(4). pii: jcs

Insert

EB2-GFP-6xHis (Mapre2 Mouse)

Use

Tags

eGFP-6xHis

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pBABE-ProDOL

Plasmid

#206866

Purpose

Expression of ProDOL probe - LynAnchor-eGFP-SNAP-tag-HaloTag

Depositor

Dirk-Peter Herten

Article

Tashev et al Nat Methods (2024)

Insert

ProDOL

Use

Retroviral

Tags

HaloTag, His-Tag, SNAP-tag, and eGFP

Expression

Mammalian

Mutation

Contains Lyn amino acids 1-16

Promoter

Available since

Aug. 8, 2024

Availability

Academic Institutions and Nonprofits only

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cpVenus-Gγ2

Plasmid

#69626

Purpose

The heterotrimeric G-protein subunit, Gγ2, tagged with circular permutated Venus (cp173V).

Depositor

Dorus Gadella

Article

van Unen et al PLoS One. 2016 Jan 22;11(1):e0146789. do

Insert

cp173Venus-Ggamma2 (GNG2 Synthetic)

Use

Tags

ciruclar permutated Venus (cp173V)

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 25, 2016

Availability

Academic Institutions and Nonprofits only

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TARDBP

Plasmid

#156073

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

TARDBP (TARDBP Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 8, 2022

Availability

Academic Institutions and Nonprofits only

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pCAGImC_V5-CSNK1A1

Plasmid

#92014

Purpose

Expresses V5-CSNK1A1, includes IRES-mCherry

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-TRE3_ mTFP1- Vamp2

Plasmid

#201214

Purpose

AAV expression of TRE(TRE3G)-driven, Vamp2-conjugated mTFP1 expression for fluorescent labling of axon terminals

Depositor

Tetsuo Yamamori

Article

Watakabe et al Neuron. 2023 May 9:S0896-6273(23)00338-0

Insert

mTFP1 (from addgene #54613)

Use

AAV

Tags

mouse Vamp2

Expression

Bacterial and Mammalian

Mutation

Promoter

TRE3G

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1-FH-AGO2-WT

Plasmid

#91978

Purpose

Expresses FLAG-HA-AGO2 (WT)

Depositor

Joshua Mendell

Article

Golden et al Nature. 2017 Feb 9;542(7640):197-202. do

Insert

AGO2 (AGO2 Human)

Use

Lentiviral

Tags

FLAG-HA

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 25, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A_BAF_G47E

Plasmid

#101774

Purpose

Expresses mutant BAF (G47E) in human cells (with EGFP produced from the same transcript as expression control)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP-P2A

Expression

Mammalian

Mutation

G47E mutation

Promoter

EF1a

Available since

Dec. 12, 2017

Availability

Academic Institutions and Nonprofits only

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pIG-831_HA-GD2-28z_CAR_TFAP4_Retroviral

Plasmid

#207508

Purpose

This plasmid can be used to generate retrovirus.

Depositor

Alexander Marson

Article

Blaeschke et al Cell. 2023 Sep 14;186(19):4216-4234.e33.

Insert

TFAP4, HA-GD2-28z_CAR (TFAP4 Human)

Use

Retroviral

Tags

Expression

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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fluoPEER-GFP>BFP

Plasmid

#185477

Purpose

Fluorescent reporter for a Y66H mutation to convert a GFP to BFP.

Depositor

Sabine Fuchs

Article

Schene et al Nat Commun. 2022 Mar 1;13(1):1028. doi:

Insert

GFP-Y66-editing-site

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMGF196

Plasmid

#97005

Purpose

Expresses LEMD2-mCherry in mammalian cells under a crippled promoter

Depositor

Wesley Sundquist, Adam Frost

Article

Gu et al Proc Natl Acad Sci U S A. 2017 Mar 14;11

Insert

LEMD2 (LEMD2 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV delta 5

Available since

Aug. 14, 2017

Availability

Academic Institutions and Nonprofits only

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pEGFP SNAP23

Plasmid

#101914

Purpose

Expresses SNAP23 in mammalian cells

Depositor

Thierry Galli

Article

Kuster et al J Biol Chem. 2015 Nov 20;290(47):28056-6

Insert

SNAP 23 (SNAP23 Human)

Use

Tags

GFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Oct. 6, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV.CMV.SV40.THBS1-deltaTSR1-HA.SV40(polyA)

Plasmid

#156162

Purpose

Expresses murine Thrombospondin-1 (THBS1) with deletion (AA 383-548) and HA-tag at C-terminus

Depositor

Kevin Park

Article

Bray et al Neuron. 2019 Aug 21;103(4):642-657.e7. d

Insert

Thrombospondin-1 (Thbs1 Mouse)

Use

AAV

Tags

HA-tag

Expression

Mammalian

Mutation

THBS1 with deletion of amino acid residues 383-548

Promoter

CMV

Available since

Aug. 21, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA5FRT-EF-Pdgfrbeta-EGFPN

Plasmid

#66790

Purpose

Expression of murine PDGFRbeta tagged with GFP under the control of EF1a promoter

Depositor

Lotte Pedersen

Article

Nielsen et al J Cell Sci. 2015 Oct 1;128(19):3543-9. d

Insert

PDGFRbeta (Pdgfrb Mouse)

Use

Tags

C-term EGFP

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

Aug. 18, 2015

Availability

Academic Institutions and Nonprofits only

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pTwist-WIV1-CoV Δ18

Plasmid

#164439

Purpose

Encodes WIV1-CoV Spike Protein lacking 18 C-terminal amino acids for pseudovirus production

Depositor

Alejandro Balazs

Article

Garcia-Beltran et al Cell. 2021 Jan 21;184(2):476-488.e11. do

Insert

WIV1-CoV Spike truncated to remove 18 amino acids

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 24, 2021

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapsin1.OxLight-ctr

Plasmid

#169793

Purpose

Control non-binding fluorescent reporter for orexin neuropeptides

Depositor

Tommaso Patriarchi

Article

Duffet et al Nat Methods. 2022 Feb;19(2):231-241. doi

Insert

OxLight-ctr

Use

AAV

Tags

Expression

Mutation

E54K; T111A

Promoter

human Synapsin-1

Available since

Feb. 11, 2022

Availability

Academic Institutions and Nonprofits only

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ADD1

Plasmid

#155397

Purpose

For use in RBP tethering screen

Depositor

Eugene Yeo

Article

Luo et al Nat Struct Mol Biol. 2020 Oct;27(10):989

Insert

ADD1 (ADD1 Human)

Use

Tags

V5-MCP

Expression

Mammalian

Mutation

none

Promoter

EF-1a

Available since

Nov. 4, 2022

Availability

Academic Institutions and Nonprofits only

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Integrin beta5-2XEGFP

Plasmid

#139779

Purpose

Detecting Integrin beta5 by fluorescence microscopy

Depositor

Staffan Stromblad

Article

Lock et al Nat Cell Biol. 2018 Nov;20(11):1290-1302

Insert

Integrin subunit beta5 (ITGB5 Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

April 24, 2020

Availability

Academic Institutions and Nonprofits only

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pCAGGS_HA-VP35_EBOV

Plasmid

#103053

Purpose

Mammalian expression vector encoding HA-tagged Ebola virus VP35 gene under control of the CAG promoter

Depositor

Elke Mühlberger

Article

Nelson et al J Virol. 2016 Jul 27;90(16):7268-84. doi

Insert

VP35 (VP35 Zaire ebolavirus, Mayinga)

Use

Tags

HA-tag

Expression

Mammalian

Mutation

Promoter

CAG

Available since

Dec. 20, 2017

Availability

Academic Institutions and Nonprofits only

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EGFP-P2A_BAF

Plasmid

#101773

Purpose

Expresses BAF in human cells (with EGFP produced from the same transcript as expression control)

Depositor

Daniel Gerlich

Article

Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

Insert

BAF (BANF1) (BANF1 Human)

Use

Lentiviral

Tags

EGFP-P2A

Expression

Mammalian

Mutation

Promoter

EF1a

Available since

March 5, 2018

Availability

Academic Institutions and Nonprofits only

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