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Plasmid#172490PurposeEncodes the anti-GFP LaG-9 nanobody with a cysteine near the C-terminus, LaG-9(S151C).DepositorInsertLaG9(S151C)
UseTags6xHis, PPX cleavage site, and pelB leader sequenc…ExpressionBacterialMutationS151CPromoterT7Available sinceMarch 7, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm1_A
Plasmid#145886PurposeInsect Expression of Dm-Lsm1DepositorInsertDm-Lsm1
UseTagsExpressionInsectMutationTwo silent mutation compared to the sequence give…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm4_A
Plasmid#145889PurposeInsect Expression of Dm-Lsm4DepositorInsertDm-Lsm4
UseTagsExpressionInsectMutationone non silent mutation G127R compared to the seq…PromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm7_A
Plasmid#145891PurposeInsect Expression of DmLSm7DepositorInsertDmLSm7 (LSm7 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceFeb. 25, 2022AvailabilityAcademic Institutions and Nonprofits only -
pUC19-pleCopyCatcher
Plasmid#174061PurposeCopyCatcher insertion donor for the pale locusDepositorInsertPale (Pale Fly, Synthetic)
UseTagsExpressionBacterialMutationPromoterAvailable sinceSept. 13, 2021AvailabilityAcademic Institutions and Nonprofits only -
UUUUA-pHG165c
Plasmid#170107PurposeConstitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the UreR urea ligand binding domain.DepositorInsertUUUUA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
MMMMA-pHG165c
Plasmid#170108PurposeConstitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the MelR L-melibiose ligand binding domain.DepositorInsertMelR
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
XXXXA-pHG165c
Plasmid#170110PurposeConstitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.DepositorInsertXXXXA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
XXXXA_Q182A_R287H-pHG165c
Plasmid#170111PurposeConstitutively expressed , mutationally-enhanced chimeric transcription activator comprising the AraC DNA binding domain and the XylS benzoate ligand binding domain.DepositorInsertXXXXA_Q182A_R278H
UseTagsExpressionBacterialMutationQ182A, R287HPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
RRRAA-pHG165c
Plasmid#170112PurposeConstitutively expressed, chimeric transcription activator comprising the AraC DNA binding domain and the RhaR rhamnose ligand binding domains.DepositorInsertRRRAA
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
AraC-pHG165c WT
Plasmid#170114PurposeConstitutively expressed, wild-type E coli AraC transcription activator; activated by L-Arabinose.DepositorInsertAraC
UseTagsExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pAc5.1B-lambdaN-HA-DmLSm3_A
Plasmid#145888PurposeInsect Expression of DmLSm3DepositorInsertDmLSm3 (LSm3 Fly)
UseTagsExpressionInsectMutationPromoterAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(2)
Plasmid#164271PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 7 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Nup54 no FG-mEGFP494(1)
Plasmid#164270PurposeMammalian expression of Nup54 without the FG-Nup domain, with mEGFP (missing the first 5 amino acids) at amino acid position 494 with a rigid alpha helix of 6 amino acids at the carboxyl end of mEGFPDepositorInsertNup54 (NUP54 Human)
UseTagsmEGFPExpressionMammalianMutationdeleted the FG region of Nup54PromoterCMVAvailable sinceFeb. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
pYPQ265H
Plasmid#149554PurposeGateway entry clone for CRISPR-iSpyMacCas9 hAID C-T base editing at NAAR PAMsDepositorInserthAID-ziSpyMacCas9(D10A)-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceAug. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ266B
Plasmid#149555PurposeGateway entry clone for CRISPR-iSpyMacCas9 PmCDA1 C-T base editing at NAAR PAMsDepositorInsertziSpyMacCas9(D10A)-PmCDA1-UGI
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
pYPQ262m3
Plasmid#149556PurposeGateway entry clone for CRISPR-iSpyMacCas9 ABE7.10 A-G base editing at NAAR PAMsDepositorInsertecTadAwt-ecTadAmut-ziSpyMacCas9(D10A)
UseCRISPRTagsExpressionPlantMutationD10A, R221K, N394K, Protospacer interacting domai…PromoterAvailable sinceJuly 20, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-C3H
Plasmid#133386PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
BII-B3HTK
Plasmid#133389PurposePiggybac vector for SRIRACCHA-mediated mutation enrichment (surrogate target for HDR)DepositorTypeEmpty backboneUseTagsExpressionMammalianMutationPromoterAvailable sinceJan. 3, 2020AvailabilityAcademic Institutions and Nonprofits only -
GB1-His-TET1-878-911-L900A(pL91)
Plasmid#124395PurposeE.Coli produced TET1-SID mutants (L900A) purified by FPLC nickel columnDepositorInsertGB1-His-TET1-878-911-L900A (TET1 Human)
UseTagsGB1-HistidineExpressionBacterialMutationL900APromoterAvailable sinceMay 23, 2019AvailabilityAcademic Institutions and Nonprofits only