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Plasmid#29653DepositorTypeEmpty backboneUseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJune 7, 2011AvailabilityAcademic Institutions and Nonprofits only
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pET264-pUC 24xMS2V6 Loxp KANr Loxp
Plasmid#104393PurposeYeast endogenous mRNA taggingDepositorInsert24xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8r
Plasmid#208318PurposeOverxpression MBP-TadA8rDepositorInsertTadA8r
UseTagsExpressionBacterialMutationmutations in TadA are described in the paperPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET Flag TEV LIC cloning vector (2L-T)
Plasmid#29715DepositorTypeEmpty backboneUseTagsFLAG-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET296-YcpLac111 CYC1p-MCP-NLS-2xyeGFP
Plasmid#104394PurposeYeast MCP-GFP expressionDepositorInsertMCP-NLS-2xyeGFP
UseTagsExpressionBacterial and YeastMutationMCP fused to NLS(SV40) and 2x yeast codon optimiz…PromoterCYC1Available sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8.20
Plasmid#194702PurposeExpresses N-terminal MBP fused TadA8.20 in bacteriaDepositorInsertTadA8.20
UseTags6xHis and MBPExpressionBacterialMutationmutations in TadA are described in the paperPromoterT7 promoterAvailable sinceFeb. 6, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET28a 6xHis PreScission SARS-CoV-2 nsp13
Plasmid#159390PurposeExpresses the SARS-CoV-2 nsp13 protein with an N-terminal His6 tag followed by an HRV 3C/PreScission cleavage site.DepositorInsertN-terminal His tag PreScission SARS-CoV-2 nsp13, optimized for insect cell expression
UseTagsHis6 tagExpressionBacterialMutationPromoterT7Available sinceSept. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
VR122 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198467PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET11a_His-TEV-PLpro (nsp3) (SARS-CoV-2)
Plasmid#169192PurposeTo express SARS-CoV-2 Papain-like protease in E. coliDepositorInsert6His-TEV-nsp3_E746-K1060 (pp1ab_E1564-K1878) (ORF1ab SARS-CoV-2, Synthetic)
UseUnspecifiedTags6His-TEVExpressionMutationCodon optimised for E. coliPromoterT7Available sinceJuly 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET28-SpRY-HF1-NLS-6xHis (gbr2102)
Plasmid#181744Purposebacterial expression plasmid for SpRY-HF1 with an n-terminal MKIEE MBP tag, and c-terminal bi-partite NLS and 6x-His tagDepositorInsertbacterial codon optimized SpRY-HF1
UseIn vitro transcription; t7 promoterTagsBPNLS(SV40)-6xHis and MKIEE MBP tagExpressionBacterialMutationSpRY-HF1, SpRY=A61R/L1111R/D1135L/S1136W/G1218K/E…PromoterT7Available sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-His-MBP-NLS-RfxCas13d-NLS-HA
Plasmid#171586PurposeBacterial expression of NLS-RfxCas13d-NLS-HADepositorInsertHis-MBP-NLS-RfxCas13d-NLS-HA
UseTagsHAExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28-MKIEE-NLS-RfxCas13d-NLS-HA-6His
Plasmid#171587PurposeBacterial expression of NLS-RfxCas13d-NLS 6HisDepositorInsertMKIEE-NLS-RfxCas13d-NLS-HA-6His
UseTagsHA-6HIS and MKIEEExpressionBacterialMutationPromoterAvailable sinceOct. 12, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET mCherry LIC cloning vector (u-mCherry)
Plasmid#29769DepositorTypeEmpty backboneUseTagsmCherryExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
HIV-1 integrase F185K/C280S in pET-15b
Plasmid#61669PurposeHIV-1 integrase F185K/C280S in pET-15bDepositorInsertHIV-1 integrase F185K/C280S
UseSynthetic BiologyTagsHisExpressionMutationPromoterT7 promotorAvailable sinceFeb. 11, 2015AvailabilityAcademic Institutions and Nonprofits only -
pET251-pUC 12xMS2V6 Loxp KANr Loxp
Plasmid#104392PurposeYeast endogenous mRNA taggingDepositorInsert12xMS2V6
UseTagsExpressionBacterialMutationAll Loops are U-variants, interspaced by 50 nucle…PromoterAvailable sinceJan. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET28a-6xHis-MBP-TEV site-TadA8e
Plasmid#208319PurposeOverxpression MBP-TadA8eDepositorInsertTadA8e
UseTagsExpressionBacterialMutationmutations in TadA are described in the paperPromoterAvailable sinceJan. 2, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-Caspase-3(D3A: D175A D28A D9A)
Plasmid#90090PurposeExpresses caspase 3 D3A mutant (D175A D28A D9A) in bacterial cellsDepositorInsertCaspase 3 (CASP3 Human)
UseTagsHis tagExpressionBacterialMutationD175A D28A D9APromoterAvailable sinceMay 2, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET mOrange LIC cloning vector (u-mOrange)
Plasmid#29770DepositorTypeEmpty backboneUseTagsmOrangeExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET His6 TEV LIC cloning vector (2B-T)
Plasmid#29666DepositorTypeEmpty backboneUseTagsHis6-TEVExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET mCerulean LIC cloning vector (H6-mCerulean)
Plasmid#29726DepositorTypeEmpty backboneUseTagsBiotin - His6 - TEV and mCeruleanExpressionBacterialMutationPromoterAvailable sinceNov. 15, 2011AvailabilityAcademic Institutions and Nonprofits only