Addgene

We narrowed to 6,285 results for: sms

Showing: 3601 - 3620 of 6285 results

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pAPM-miR30-DDX3X-ts3

Plasmid

#174237

Purpose

DDX3X knockdown

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

DDX3X shRNA (DDX3X Human)

Use

Lentiviral and RNAi

Tags

Expression

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-huMDA5-miR30-L1221

Plasmid

#174223

Purpose

All-in-one huMDA5 rescue-control shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pPU-huMDA5-miR30-MDA5

Plasmid

#174224

Purpose

All-in-one huMDA5 rescue-MDA5 shRNA knockdown vector

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MDA5delta2CARD

Plasmid

#174225

Purpose

Expresses human MDA5 that lacks 2 CARD domains

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

deletion of 2CARD

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MDA5-S88D

Plasmid

#174226

Purpose

Expresses human MDA5 S88D mutant

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S88D

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pscALPSpuro-MDA5-S88E

Plasmid

#174227

Purpose

Expresses human MDA5 S88E mutant

Depositor

Jeremy Luban

Article

Guney et al Proc Natl Acad Sci U S A. 2024 Jul 16;12

Insert

MDA5 (IFIH1 Human)

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

S88E

Promoter

SFFV

Available since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pKE12-ISceI-fabp10a:loxP-BFP-loxP-Xla.Ctnnb1

Plasmid

#198240

Purpose

For I-SceI-mediated transgenesis in zebrafish; fabp10a promoter drives expression of activated β-catenin preceded by a blue fluorescent protein (BFP)-STOP cassette flanked by loxP sites

Depositor

Didier Stainier

Article

Kalasekar et al Biol Open. 2019 Oct 17;8(10):bio047829.

Inserts

Zebrafish fabp10a promoter:floxedBFP, Xenopus pt-beta-catenin coding sequence, polyA (fabp10a Frog)
Zebrafish cryaa promoter:mcherry fluorescent protein coding sequence (cryaa Zebrafish)

Use

Zebrafish transgenesis

Tags

Expression

Mutation

Promoter

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pENN491_TALEcharm_MmT8x

Plasmid

#220836

Purpose

TALEcharm Kv2 expression in mammalian cells; targets Prnp

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

TALEcharm

Use

Lentiviral and TALEN

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EF1a

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pENN456_TALEcharm_Kv2_MmT8x

Plasmid

#220835

Purpose

TALEcharm expression in mammalian cells

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

TALEcharm Kv2

Use

Lentiviral and TALEN

Tags

P2A-TagBFP

Expression

Mammalian

Mutation

N/A

Promoter

EF1a

Available since

July 3, 2024

Availability

Academic Institutions and Nonprofits only

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pTMB398_ZFcharm_Prnp_SMM

Plasmid

#220845

Purpose

AAV genome expressing ZFcharm targeting Prnp; single mismatch self-silencing binding site

Depositor

Jonathan Weissman

Article

Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

Insert

ZFcharm

Use

AAV

Tags

HAtag

Expression

Mammalian

Mutation

N/A

Promoter

EFS

Available since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-bIV spectrin (repeats 13-15)

Plasmid

#213728

Purpose

Mammalian cell expression vector for overexpression of bIV spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bIV spectrin (repeats 13-15) (Sptbn4 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-3xFLAG-bII spectrin (repeats 13-15)

Plasmid

#213729

Purpose

Mammalian cell expression vector for overexpression of bII spectrin (repeats 13-15) in NIH/3T3 cells.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

bII spectrin (repeats 13-15) (Spnb3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AnkG ZU5in-3xHA

Plasmid

#213730

Purpose

Mammalian cell expression vector for overexpression of AnkG ZU5in isoform in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkG ZU5in (Ank3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AnkB ZU5ex-3xHA

Plasmid

#213732

Purpose

Mammalian cell expression vector for overexpression of AnkB ZU5ex isoform in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkB ZU5ex (Ank2 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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pCAGGS-AnkG ZU5ex-3xHA

Plasmid

#213731

Purpose

Mammalian cell expression vector for overexpression of AnkG ZU5ex isoform in motor neurons.

Depositor

Chaolin Zhang

Article

Jacko et al Neuron. 2018 Feb 21;97(4):853-868.e6. do

Insert

AnkG ZU5ex (Ank3 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 12, 2024

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-LUC7L2_sgRNA1

Plasmid

#201602

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L2 (LUC7L2 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Dec. 8, 2023

Availability

Academic Institutions and Nonprofits only

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TRE-UBC-Qa1 SCT-T2A-GFP

Plasmid

#205453

Purpose

lentiviral plasmid for expression of mouse Qa1 SCT

Depositor

Deepta Bhattacharya

Article

Pizzato et al Stem Cell Reports. 2023 Dec 22:S2213-671

Insert

Qa1 SCT (H2-T23 Mouse)

Use

Tags

Expression

Mammalian

Mutation

Promoter

UBC

Available since

Nov. 8, 2023

Availability

Academic Institutions and Nonprofits only

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pLV_PGK_3xHA-LTB4R(T308A,S310A)

Plasmid

#208094

Purpose

Mammalian expression of the human LTB4 receptor LTB4R with the T308A and S310A mutations with an N-terminal 3x HA tag.

Depositor

Sean R Collins

Article

Lundgren et al Sci Signal. 2023 Oct 3;16(805):eadd1845.

Insert

3xHA-LTB4R(T308A,S310A) (LTB4R Human)

Use

Lentiviral

Tags

3xHA

Expression

Mammalian

Mutation

T308A, S310A

Promoter

PGK

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pROF148

Plasmid

#155330

Purpose

Binary vector for expression of the PULSE system under the control of AtUbi10 promoter. It contains a plant selection cassette.

Depositor

Matias Zurbriggen

Article

Ochoa-Fernandez et al Nat Methods. 2020 Jul;17(7):717-725. doi

Insert

LB_Tnos-nptII-Pnos_PAtUbi10-SRDX-NLS-EL222-Tnos_PAtUbi10-E-PIF6-NLS-Tnos_PAtUbi10-PhyB-VP16-NLS-Tnos_RB

Use

Synthetic Biology

Tags

Expression

Plant

Mutation

Promoter

Available since

Oct. 26, 2023

Availability

Academic Institutions and Nonprofits only

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pWPI-LUC7L-3xFLAG

Plasmid

#201640

Purpose

Over-expression of 3xFLAG-tagged human LUC7L

Depositor

Vamsi Mootha, Alexis Jourdain

Article

Jourdain et al Mol Cell. 2021 May 6;81(9):1905-1919.e12

Insert

LUC7L (LUC7L Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mutation

Promoter

Available since

July 26, 2023

Availability

Academic Institutions and Nonprofits only

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