Addgene

We narrowed to 6,564 results for: ski

Showing: 3601 - 3620 of 6564 results

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pBabe-PI(WT)-Rac1(Q61L)-mCherry

Plasmid

#87553

Purpose

PI(WT): Photo-inhibitable/blue light sensitive; const. active Rac1

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Rac1 (RAC1 Human)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Q61L

Promoter

CMV

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mCherry-T2A-miniSOG-C-Far

Plasmid

#193915

Purpose

Expresses miniSOG targeted to the plasma membrane of neurons, joined by a T2A cleavage site with mCherry; for AAV production

Depositor

Xiao Wang

Article

Sessler et al Sci Adv. 2022 Dec 9;8(49):eade1136. doi:

Insert

miniSOG

Use

AAV

Tags

Farnesylation signal sequence and Flag tag

Expression

Mammalian

Mutation

Promoter

hSyn

Available since

Jan. 24, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM1 FRB-myc-RagC

Plasmid

#112741

Purpose

express FRB-myc-RagC

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

FRB-myc-RagC (RRAGC Human)

Use

Lentiviral

Tags

FRB, myc

Expression

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX ShcA-SH2

Plasmid

#46486

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

ShcA (SHC1 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 476-584

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago2_4

Plasmid

#73532

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX Nck-SH2

Plasmid

#46457

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Nck (NCK1 Human)

Use

Tags

GST and Thrombin site

Expression

Bacterial

Mutation

contains amino acids 275-377

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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ama1CD4d3+4-blac

Plasmid

#36146

Purpose

EXPRESs plasmid for P. falciuparum merozoite surface proteins encoding P. falciparum ama1 with rat CD4d3+4-blac

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ama1 (PF3D7_1133400 Plasmodium falciparum)

Use

Tags

beta-lactamase, pentamerization domain of rat car…

Expression

Mammalian

Mutation

Promoter

CMV

Available since

May 9, 2012

Availability

Academic Institutions and Nonprofits only

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pPHR-mCherry-NLS

Plasmid

#103819

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-mCherry-NLS (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 30, 2017

Availability

Academic Institutions and Nonprofits only

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pPHR-EYFP-NLS

Plasmid

#103820

Purpose

Soluble BLInCR mock effector that is recruited to 'localizer' sites upon blue light illumination

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

PHR-EYFP-NLS (CRY2 Mustard Weed)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Dec. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pNAS1b split mCherry NEDD4-2 WW2

Plasmid

#111883

Purpose

Mode #2 phosphosite expression (split mCherry, using NEDD4-2 WW2 binding domain)

Depositor

Jesse Rinehart

Article

Barber et al Nat Biotechnol. 2018 Aug;36(7):638-644.

Type

Empty backbone

Use

Tags

Split mCherry system: NEDD4-2 WW2 fused to C-term…

Expression

Bacterial

Mutation

Promoter

Available since

June 20, 2018

Availability

Academic Institutions and Nonprofits only

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pGEX SH2A-SH2

Plasmid

#46481

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

SH2A (SH2D4A Human)

Use

Tags

AviTag, GST, and PreScission

Expression

Bacterial

Mutation

contains amino acids 338-445

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.mTagBFP2.let-7c

Plasmid

#169310

Purpose

Constitutive overexpression of let-7c with mTagBFP2 as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

let-7c (MIRLET7C Human)

Use

Lentiviral

Tags

TagBFP2

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dTomato.let-7c

Plasmid

#169301

Purpose

Constitutive overexpression of let-7c with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

let-7c (MIRLET7C Human)

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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SIN40C.SFFV.dTomato.miR-125b-2

Plasmid

#169302

Purpose

Constitutive overexpression of miR-125b-2 with dTomato as a reporter fluorescent protein

Depositor

Jan-Henning Klusmann

Article

Alejo-Valle et al Blood. 2021 Sep 27. pii: S0006-4971(21)0

Insert

miR-125b-2 (MIR125B2 Human)

Use

Lentiviral

Tags

dTomato

Expression

Mutation

Promoter

SFFV

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pBabe_puro_DEST_Flag_PRKCI

Plasmid

#45263

Depositor

William Hahn

Article

Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

Insert

PKCI (PRKCI Human)

Use

Retroviral

Tags

Flag

Expression

Mammalian

Mutation

Promoter

Available since

June 14, 2013

Availability

Academic Institutions and Nonprofits only
pGEX Slap2-SH2

Plasmid

#46501

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Slap2 (SLA2 Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 84-197

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pGEX Lnk-SH2

Plasmid

#46451

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Lnk (Sh2b3 Mouse)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 325-466

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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Rescue Septin 11 mRNA

Plasmid

#122022

Purpose

Rescue mRNA for Septin 11 Knock Down with sh1 shRNA. Splice variant II (EU711415) with 5 silent mutations at the SEPT11 sh1 shRNA target site.

Depositor

Angel de Blas

Article

Li et al J Biol Chem. 2009 Jun 19;284(25):17253-6

Insert

SEPT11 (Sept11 Rat)

Use

Tags

Expression

Mammalian

Mutation

Silent mutations are in 5 consecutive codons (AUU…

Promoter

Available since

Feb. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pSECB sgMTHFD2L_1

Plasmid

#106306

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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pSECB sgMTHFD2L_2

Plasmid

#106307

Purpose

Express Cas9 and sgRNA targeting MTHFD2L

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

sgRNA targeting MTHFD2L (MTHFD2L Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 27, 2018

Availability

Academic Institutions and Nonprofits only

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