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Plasmid#60349PurposeH83F mutant of the mouse MSI1 RBDDepositorInsertmouse MSI1 (7-192) (Msi1 Mouse)
UseTagsHis and TEV protease siteExpressionBacterialMutationH83F; contains amino acids 7-192 of Msi1PromoterT7Available sinceOct. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET28a-ybbR-HIS-Spec(x2)-DocI
Plasmid#58713PurposeCharacteristic fingerprint molecule to perform single molecule force spectroscopy experiments; covalently immobilized via ybbr tagDepositorInsertSpec(x2)-DocI
UseTagsHIS, HRV 3C, and ybbRExpressionBacterialMutationPromoterT7Available sinceAug. 25, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-851 A50I mutation
Plasmid#46174DepositorInsertVcl 1-851 A50I (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-851 A50I mutation (talin binding domain mut…PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET15b/GgVcl 1-258 A50I mutation
Plasmid#46175DepositorInsertVcl 1-258 A50I (VCL Chicken)
UseTagsHisExpressionBacterialMutationaa 1-258 (VD1) A50I mutation; talin binding defic…PromoterT7Available sinceJuly 10, 2013AvailabilityAcademic Institutions and Nonprofits only -
pET28b(+)_S. cerevisiae Hbs1(162-611)
Plasmid#39337DepositorInsertElongation factor 1 alpha-like protein
UseTagsHis-tagExpressionBacterialMutationPromoterT7Available sinceSept. 27, 2012AvailabilityAcademic Institutions and Nonprofits only -
pET-21a(+)-DR RecR(K23A/R27A)
Plasmid#12619DepositorInsertDeinococcus radiodurans RecR (recR Deinococcus radiodurans)
UseTagsHisExpressionBacterialMutationchanged Lysine 23 to Alanine, and Arginine 27 to …PromoterAvailable sinceJan. 5, 2007AvailabilityAcademic Institutions and Nonprofits only -
p141 pETF-GST RanBP10 G308R
Plasmid#11094DepositorInsertRanBP10 (Ranbp10 Mouse)
UseTagsGSTExpressionBacterialMutationG308RPromoterAvailable sinceDec. 16, 2005AvailabilityAcademic Institutions and Nonprofits only -
Kv7.4 BD (522-645, C643A)/pET28HMT
Plasmid#111073PurposeProtein expression in E.ColiDepositorInsertpotassium voltage-gated channel subfamily KQT member 4 isoform a [Homo sapiens] (KCNQ4 Human)
UseTagsHis Tag and MBP tagExpressionBacterialMutationKv7.4 BD (522-645, C643A)PromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
Pet28a His6-HSPB7 (zebrafish) C117S
Plasmid#110072PurposeExpress C117S mutant of Zebrafish His6-HSPB7 in bacteriaDepositorInsertHis6-HSPB7 (hspb7 Zebrafish)
UseTagsHis6ExpressionBacterialMutationCysteine 117 to SerinePromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET His10-Draper delta ITAM
Plasmid#126671PurposeExpression of His10-Draper delta ITAM mutant for purification from E. coliDepositorInsertDraper cytoplasmic ITAM residues mutated to Phe
UseTagsExpressionBacterialMutationCytoplasmic ITAM residues mutated to PhePromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B1-DK
Plasmid#117981PurposeSdrG B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B1
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrG_B2-DK
Plasmid#117982PurposeSdrG B2 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET28a-MGGG-ybbr-HIS-SdrD_B1-DK
Plasmid#117983PurposeSdrD B1 domain with N-terminal ybbr and sortase tags for covalent immobilization and C-terminal DK peptide for thethering with ClfBDepositorInsertSdrG_B2
UseTagsHIS, sortase, and ybbrExpressionBacterialMutationPromoterT7Available sinceAvailabilityAcademic Institutions and Nonprofits only -
pET-Duet1-His-TEV-mCherry-p62
Plasmid#199780PurposeUsed for the expression of mCherry labelled p62 (SMC Internal No. 391).DepositorInsertp62 (NUP62 Human)
UseTags6x His, TEV, mCherryExpressionBacterialMutationPromoterAvailable sinceApril 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET GFP LIC cloning vector (2GFP-T)
Plasmid#29716DepositorTypeEmpty backboneUseTagsHis6-GFP-TEVExpressionBacterialMutationPromoterAvailable sinceAug. 17, 2011AvailabilityAcademic Institutions and Nonprofits only -
pET GFP LIC cloning vector (u-msfGFP)
Plasmid#29772DepositorTypeEmpty backboneUseTagsGFPExpressionBacterialMutationPromoterAvailable sinceJune 9, 2011AvailabilityAcademic Institutions and Nonprofits only -
pETM6-G6-vioABE-4A6-vioC-3A2-vioD
Plasmid#73440PurposeViolacein pathway (vioABECD) in monocistronic configuration, transcriptionally driven by orthogonal T7-lac promoter variants G6 (vioABE), 4A6 (vioC), and 3A2 (vioD) for orthogonal flux redirection.DepositorInsertPG6-vioABE-P4A6-vioC-P3A2-vioD
UseCRISPR and Synthetic BiologyTagsExpressionBacterialMutationPromoterPG6, P4A6, and P3A2 (orthogonal T7-lac variants)Available sinceMarch 1, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET His6 LIC cloning vector (2Bc-T)
Plasmid#37236DepositorTypeEmpty backboneUseTagsHis6ExpressionBacterialMutationPromoterT7Available sinceAug. 2, 2012AvailabilityAcademic Institutions and Nonprofits only -
p36-p37-p38-p40-pET-Duet-1
Plasmid#175049PurposeOverexpression of human Rfc2,Rfc3,Rfc4,Rfc5 in E.coliDepositorUseTagsExpressionBacterialMutationPromoterT7 promoterAvailable sinceSept. 24, 2021AvailabilityAcademic Institutions and Nonprofits only -
VR124 pETv2 10His-pG-Tn5 (E54K, L372P)
Plasmid#198468PurposeE. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.DepositorInsert10His-pG-Tn5 (E54K, L372P)
UseTags10His-pGExpressionBacterialMutationE54K, L372PPromoterConsensus φ10 T7 PromoterAvailable sinceApril 14, 2023AvailabilityAcademic Institutions and Nonprofits only