We narrowed to 5,856 results for: pCas

1. p2T-U6-sgCTG

   Plasmid

   #232723

   Purpose

   SpCas9 guide RNA targeting CAG repeats for C-to-T base editing

   Depositor

   David Liu

   Article

   Matuszek et al Nat Genet. 2025 May 26. doi: 10.1038/s41

   Insert

   SpCas9 gRNA targeting CAG repeats
   

   Expression

   Mammalian

   Available Since

   March 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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2. pGES401

   Plasmid

   #190198

   Purpose

   For CRISPR-Cas9 mediated multiple gene editing in soybean, single transcript unit system driven by soybean elongation factor 1A promoter (pM4), Basta selection

   Depositor

   Yuefeng Guan

   Article

   Bai et al Mol Plant. 2022 Jul 4;15(7):1081-1083. d

   Insert

   spCas9
   

   Use

   CRISPR

   Tags

   3xFlag

   Expression

   Plant

   Mutation

   plant-codon optimized

   Promoter

   Glycine max elongation factor 1A(pM4)

   Available Since

   Sept. 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pMSCVpuro-Gcn5

   Plasmid

   #63706

   Purpose

   retroviral expression of mouse Gcn5 in mammalian cells

   Depositor

   Kai Ge

   Article

   Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

   Insert

   Gcn5 (Kat2a Mouse)
   

   Use

   Retroviral

   Tags

   None

   Expression

   Mammalian

   Available Since

   April 8, 2015

   Availability

   Academic Institutions and Nonprofits only

4. pcDNA™5/FRT/CMV_promoter-Cas9-E2A-mRFP

   Plasmid

   #164131

   Purpose

   Expresses SpCas9 and mRFP in mammalian cells (an insertion vector to generate Cas9-knockin cell lines)

   Depositor

   Hyongbum Kim

   Article

   Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

   Insert

   SpCas9 (cas9 Synthetic)
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Feb. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCC_09 - hU6-BsmBI-sgRNA(E+F)-barcode-EFS-KRAB-dCas9-NLS-2A-Puro-WPRE

   Plasmid

   #139094

   Purpose

   Expresses human codon-optimized inactive SpCas9 fused to a transcriptional repressor KRAB in mammalian cells. For cloning of sgRNAs using BsmBI. Contains a barcode downstream of sgRNA cassette.

   Depositor

   Neville Sanjana

   Article

   Legut et al Cell Rep. 2020 Mar 3;30(9):2859-2868.e5.

   Insert

   KRAB-dSpCas9
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   D10A, H840A

   Available Since

   March 6, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX335-NQL002-WAPL-sgRNA1

   Plasmid

   #175550

   Purpose

   For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL003-WAPL-sgRNA2 targeting construct.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nickase and sgRNA against mouse WAPL STOP Codon
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pX335-NQL003-WAPL-sgRNA2

   Plasmid

   #175551

   Purpose

   For transient expression of spCas9-nickase and one sgRNA targeting the mouse WAPL locus. Use together with pX335-NQL002-WAPL-sgRNA1 targeting construct.

   Depositor

   Elzo de Wit

   Article

   Liu et al Nat Genet. 2021 Jan;53(1):100-109. doi:

   Insert

   spCas9-nickase and sgRNA against mouse WAPL STOP Codon
   

   Use

   Mouse Targeting

   Expression

   Mammalian

   Available Since

   Nov. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2440

   Plasmid

   #72250

   Purpose

   Human expression plasmid for SpCas9-VQR-HF1 variant: CMV-T7-humanSpCas9-VQR-HF1(N497A, R661A, Q695A, Q926A, D1135V, R1335Q, T1337R)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 VQR-HF1(N497A/R661A/Q695A/Q926A/D1135V/R1335Q/T1337R)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, D1135V, R1335Q, and T…

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. pEJS-HZ96 AAV-SpABE8e-N-terminus_tracrRNA

   Plasmid

   #211817

   Purpose

   AAV vector expressing N-terminal of SpCas9-ABE8e and tracrRNA

   Depositor

   Erik Sontheimer

   Article

   Zhang et al Nucleic Acids Res. 2023 Nov 30:gkad1125.

   Insert

   N-terminus of SpCas9-ABE8e and tracrRNA
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   chicken β-actin promoter and U6 promoter

   Available Since

   Jan. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. SECURE miniABEmax-V82G (pJUL1828)

    Plasmid

    #131313

    Purpose

    CMV promoter expression plasmid for bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS (miniABEmax with V82G mutation).

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    bpNLS-TadA7.10(V82G)-32AA linker-hSpCas9n(D10A)-bpNLS-P2A-EGFP-NLS
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Sept. 28, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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11. pLenti-EF1a-SPdCas9-DNMT3A-2A-Blast

    Plasmid

    #71216

    Purpose

    dCas9 fused to human DNMT3A catalytic domain

    Depositor

    Yonglun Luo

    Article

    Lin et al Gigascience. 2018 Mar 1;7(3):1-19. doi:

    Insert

    dSpCas9-DNMT3A catalytic domain
    

    Use

    Lentiviral

    Tags

    dSpCas9-DNMT3A

    Expression

    Mammalian

    Available Since

    June 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. hA3A-BE3 (pRZ215)

    Plasmid

    #131314

    Purpose

    CAG promoter expression plasmid for hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hA3A-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pMSCVpuro-Gcn5(D608A)

    Plasmid

    #63707

    Purpose

    retroviral expression of enzyme-dead mouse Gcn5 in mammalian cells

    Depositor

    Kai Ge

    Article

    Jin et al EMBO Rep. 2014 Nov;15(11):1192-201. doi:

    Insert

    Gcn5 (Kat2a Mouse)
    

    Use

    Retroviral

    Tags

    None

    Expression

    Mammalian

    Mutation

    D608A mutation

    Available Since

    April 8, 2015

    Availability

    Academic Institutions and Nonprofits only

14. pCK668

    Plasmid

    #192637

    Purpose

    Expresses dCas9-NG and MCP-SoxS(R93A/S101A) on p15A-CmR

    Depositor

    Jesse Zalatan

    Article

    Kiattisewee et al ACS Synth Biol. 2022 Nov 15. doi: 10.102

    Inserts

    Sp.pCas9-dCas9-NG
    BBa_J23107-MCP-SoxS(R93A, S101A)
    

    Use

    CRISPR and Synthetic Biology

    Mutation

    SoxS has R93A and S101A mutations and dCas9-NG ha…

    Promoter

    BBa_J23107 and Sp.pCas9

    Available Since

    Jan. 10, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. lenti-Cas9-VQR-Blast

    Plasmid

    #87155

    Purpose

    lentiviral expression of SpCas9-VQR (NGA PAM restricted)

    Depositor

    Daniel Bauer

    Article

    Canver et al Nat Genet. 2017 Feb 20. doi: 10.1038/ng.

    Insert

    SpCas9-VQR(D1135V,R1335Q,T1337R)
    

    Use

    Lentiviral

    Mutation

    D1135V,R1335Q,T1337R

    Available Since

    Feb. 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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16. hAID-BE3 (pRZ352)

    Plasmid

    #131316

    Purpose

    CAG promoter expression plasmid for hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP.

    Depositor

    Keith Joung

    Article

    Grunewald et al Nat Biotechnol. 2019 Sep 2. pii: 10.1038

    Insert

    hAID-XTEN linker-hSpCas9n(D10A)-UGI-NLS-P2A-EGFP
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Oct. 24, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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17. LF701: pMAGIC (L1-R5) hU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121817

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty human U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLCHKO_GFP_Luciferase

    Plasmid

    #155079

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases (CHyMErA system), respectively

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting GFP and Luciferase using SpCas9 and LbCas12a nucleases
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLCHKO_TK1_HPRT1_Lb_1

    Plasmid

    #155059

    Purpose

    Lentiviral plasmid for expressing U6 driven hybrid guide (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a (CHyMErA system)

    Depositor

    Benjamin Blencowe, Jason Moffat

    Article

    Gonatopoulos-Pournatzis et al Nat Biotechnol. 2020 May;38(5):638-648.

    Insert

    (hg)RNA targeting TK1 using SpCas9 and HPRT1 using LbCas12a
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 12, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. LG002: pMAGIC (L1-R5) mU6::xCas9(3.7) gRNA scaffold

    Plasmid

    #121816

    Purpose

    pMAGIC L1-R5 entry plasmid, contains empty mouse U6-driven xCas9(3.7) (i.e. SpCas9) gRNA scaffold for 4-component MultiSite Gateway Pro assembly. Protospacer motif can be inserted after BsaI digestion

    Depositor

    Christopher Newgard

    Article

    Haldeman et al Nucleic Acids Res. 2018 Dec 27. pii: 526

    Type

    Empty backbone

    Use

    Synthetic Biology; Pmagic gateway entry plasmid

    Available Since

    June 27, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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