Addgene

We narrowed to 5,235 results for: hwa

Showing: 3601 - 3620 of 5235 results

Results per page:

Sort results by:

pHAGE-FKBP-mEGFP-WIPI2 IDR (364-425aa)

Plasmid

#223758

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI2 IDR (WIPI2 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Expression

Mutation

aa 364-425 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pHAGE-FKBP-mEGFP-WIPI2d (R108E/R125E)

Plasmid

#223770

Purpose

Stable expression and inducible recruitment of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI2d (WIPI2 Human)

Use

Lentiviral

Tags

FKBP-mEGFP

Expression

Mutation

R108E/R125E

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGenlenti_ATG13-WT

Plasmid

#223771

Purpose

Stable expression of protein in mammalian cell culture, rescue experiments

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 (ATG13 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet1_6xHis-TEV-mCherry-WIPI2d (K87A/K88A)

Plasmid

#223751

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI2d (WIPI2 Human)

Use

Tags

6xHis-TEV-mCherry

Expression

Bacterial

Mutation

K87A/K88A

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pETDuet1_6xHis-TEV-EGFP-FIP200-CTR(1429-1591aa)

Plasmid

#223724

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

FIP200-CTR (RB1CC1 Human)

Use

Tags

6xHis-TEV-EGFP

Expression

Bacterial

Mutation

aa 1429-1591 only

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1_GST-LC3C

Plasmid

#223727

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

LC3C (MAP1LC3C Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-126 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGEX-4T1_GST-GBRP

Plasmid

#223728

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

GABARAP (GABARAP Human)

Use

Tags

GST

Expression

Bacterial

Mutation

aa 2-116 only, with Glycine exposed

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGenLenti V5-NIX

Plasmid

#223731

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

NIX (BNIP3L) (BNIP3L Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pGenLenti V5-BNIP3

Plasmid

#223732

Purpose

Stable expression of protein in mammalian cell culture

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

BNIP3 (BNIP3 Human)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG_mCherry-ATG13 (WT)

Plasmid

#223735

Purpose

Expression of recombinant protein for purification

Depositor

Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG13 (ATG13 Human)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG_ATG101

Plasmid

#223796

Purpose

Expression of recombinant protein for purification (co-expression with ATG13)

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

ATG101 (ATG101 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAG_GST-TEV-WIPI1

Plasmid

#223798

Purpose

Expression of recombinant protein for purification

Depositor

James Hurley, Sascha Martens

Article

Adriaenssens et al bioRxiv [Preprint]. 2024 Aug 28:2024.08.

Insert

WIPI1 (WIPI1 Human)

Use

Tags

GST-TEV

Expression

Mammalian

Mutation

Promoter

Available since

March 25, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
shRNA_circSMAD2_2

Plasmid

#215222

Purpose

Supression of shcircSMAD2(2,7)_2 expression

Depositor

Sefi Rosenbluh

Article

Liu et al Nat Cell Biol. 2024 Aug;26(8):1359-1372.

Insert

circSMAD2 shRNA 2 (SMAD2 Human)

Use

Lentiviral

Tags

Expression

Mutation

Promoter

Available since

March 17, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pPB-EF1A-mSTING (1-339)

Plasmid

#229740

Purpose

Expresses mouse STING amino acids 1-339 in mammalian cells. Stable expression with piggybac transposase.

Depositor

Shawn Ferguson

Article

Bentley-DeSousa et al J Cell Biol. 2025 Feb 3;224(2):e20231015

Insert

STING (Sting1 Mouse)

Use

Tags

None

Expression

Mammalian

Mutation

Contains amino acids 1-339

Promoter

EF1A

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pSW031

Plasmid

#231144

Purpose

Used to express cyclic sortase cW11 (recognizes APXTG motif) from E. coli.

Depositor

Philip Cole

Article

Whedon et al J Am Chem Soc. 2024 Nov 25. doi: 10.1021

Insert

S. aureus Sortase A mutant

Use

Tags

Nostoc punctiform split intein C-terminal fragmen…

Expression

Bacterial

Mutation

P94S, E108Q, S116V, D160N, T164Q, V168M, L169H, D…

Promoter

T7

Available since

Feb. 3, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLJC2-DHFR-3xFLAG

Plasmid

#217428

Purpose

Lentiviral overexpression of human DHFR

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

DHFR (DHFR Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Mutation

Insert sequence is a codon-optimized gBLOCK (IDT)

Promoter

CMV

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgABCC4_1

Plasmid

#217433

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 1 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLentiCRISPR-v1-sgABCC4_3

Plasmid

#217434

Purpose

Lentiviral vector expressing Cas9 and an sgRNA targeting human ABCC4

Depositor

Jason Cantor

Article

Flickinger et al Sci Adv. 2024 Oct 4;10(40):eadq3591. doi

Insert

sgRNA 3 targeting ABCC4 (ABCC4 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

U6

Available since

Jan. 31, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pcDNA5 FRT TO GFP11-LRRK2

Plasmid

#231174

Purpose

Expresses GFP-11-LRRK2 (wildtype, full length) in mammalian cells

Depositor

Samara Reck-Peterson

Article

Raig et al bioRxiv [Preprint]. 2025 Feb 13:2024.09.

Insert

GFP11-LRRK2 (LRRK2 Human)

Use

Tags

GFP11

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pLKO.1_puro_shNRF2-1

Plasmid

#230086

Purpose

shRNA silencing human NRF2 gene

Depositor

Xingguo Zhu

Article

Xi et al Commun Biol. 2025 Jan 6;8(1):15. doi: 10

Insert

Nrf2 (Nuclear factor-erythroid factor 2-related factor 2) (NFE2L2 Human)

Use

Lentiviral and RNAi

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 10, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge