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Plasmid#225502PurposeInducible expression of FLAG-tagged mutated p37 proteinDepositorInsertp37 (UBXN2B Human)
UseLentiviral; Gateway destination vector, doxycycli…TagsFLAGExpressionMammalianMutationF215A/E218A/QKL220-222AAAPromoterTRE promoter, Tet ONAvailable sinceOct. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pUC57 mini-FLAG-HA-Regnase-3 WT
Plasmid#222651PurposeFor in vitro transcription of mouse Zc3h12c tagged with FLAG-HA at N-termDepositorInsertZc3h12c (Zc3h12c Mouse)
UseIn vitro transcriptionTagsFLAG and HAExpressionMutationPromoterT7Available sinceOct. 8, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCI_FLAG-GFP(Y39TAG)-6His
Plasmid#223507PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
UseTags6xHis and FLAGExpressionMammalianMutationY39TAGPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pBAD_FLAG-GFP(Y39TAG)-6His
Plasmid#223510PurposeFluorescent reporter plasmid for genetic code expansion which consists of a modified GFP with a Amber stop codon on position Y39DepositorInsertGFP(Y39TAG)
UseTags6xHis and FLAGExpressionBacterialMutationY39TAGPromoterAvailable sinceOct. 7, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS puro 3XFLAG-hATXN7L3
Plasmid#225725PurposeTransfection of ATXN7L3 with 3XFLAG tag in mammalian cellsDepositorInsertATXN7L3 (ATXN7L3 Human)
UseTags3XFLAGExpressionMammalianMutationPromoterAvailable sinceSept. 18, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/TO-FLAG-msMMS22L
Plasmid#221015Purposemammalian expression vector of Flag tagged mouse MMS22LDepositorInsertMMS22L (Mms22l Mouse)
UseTagsFlagExpressionMammalianMutationnaturally siResistant to siMMS22L (human) aagacuu…PromoterCMVAvailable sinceSept. 5, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pCMV-FLAG-p31(comet)
Plasmid#221692PurposeMammalian expression of FLAG-tagged human p31(comet)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCMV-FLAG-p31(comet)-QF
Plasmid#221693PurposeMammalian expression of FLAG-tagged human p31(comet) (Q83A/F191A)DepositorInsertMAD2BP1
UseTagsExpressionMammalianMutationQ83A, F191APromoterAvailable sinceJuly 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-Flag-RECQL5
Plasmid#222620PurposeMammalian expression vector of flag-tagged RECQL5DepositorInsertRECQL5 (RECQL5 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-FRT/T0-Flag-BRIP1
Plasmid#222619PurposeMammalian expression vector of flag-tagged BRIP1DepositorInsertBRIP1 (BRIP1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJuly 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pJL1-sFLAG-p5-PRB4_R7-9-FETCH1
Plasmid#210651PurposeIn vitro expression of polyProline FETCH reporter from the T7 promoterDepositorInsertsFLAG-p5-PRB4_R7-9-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sFLAG-p1-PRB4_R7-9-FETCH1
Plasmid#210652PurposeIn vitro expression of polyProline FETCH reporter from the T7 promoterDepositorInsertsFLAG-p1-PRB4_R7-9-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
pJL1-sFLAG-a5-PRB4_R7-9A-FETCH1
Plasmid#210653PurposeIn vitro expression of polyProline FETCH control from the T7 promoterDepositorInsertsFLAG-a5-PRB4_R7-9A-FETCH1
UseSynthetic BiologyTagsExpressionMutationPromoterT7Available sinceJuly 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
FP5369 KIF17MD-flag- FIREmate
Plasmid#216724PurposeExpresses motor domain of KIF17 fused to FIREmate in mamallian cellsDepositorInsertKIF17 (KIF17 Human)
UseTagsFIREmate and FlagExpressionMammalianMutation1-547PromoterCMVAvailable sinceJuly 10, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-FRT/T0-3xFLAG-NFATC2IP
Plasmid#221208PurposeMammalian expression vector of triple Flag tagged NFATC2IPDepositorInsertNFATC2IP (NFATC2IP Human)
UseTags3XFlagExpressionMammalianMutation1st Methionine deletedPromoterCMVAvailable sinceJuly 3, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA5-3xFLAG-TAF5-∆ex8
Plasmid#209050PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8)DepositorInsertTAF5 deltaexon-8
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-TAF5-FL_WobbleMut
Plasmid#209055PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged full length TAF5 isoform mutated to be resistant to TAF5 siRNADepositorInsertTAF5 full length
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5-3xFLAG-TAF5-∆ex8_WobbleMut
Plasmid#209056PurposeVector for generating Flp-In cell lines allowing dox-inducible mammalian expression of 3xFlag-tagged TAF5 isoform lacking the alternative exon-8 (∆ex8) mutated to be resistant to TAF5 siRNADepositorInsertTAF5 deltaexon-8
UseTagsFLAGExpressionMammalianMutationPromoterCMV/TO inducible promoterAvailable sinceJune 20, 2024AvailabilityAcademic Institutions and Nonprofits only -
pHIV-EGFP.Flag-EZH2-mt1
Plasmid#220244PurposeExpresses an EZH2 mutant for knockout/rescue experiments in mammalian cells.DepositorInsertEZH2 mt 1 (EZH2 Human)
UseLentiviralTagsFlagExpressionMammalianMutationR27A, R31A, F165APromoterEF-1αAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3-TEX264-SIM1_mut-cFlag
Plasmid#220205PurposeMammalian expression of TEX264 with SIM1 motif mutated, C-terminal FlagDepositorInsertTEX264 (TEX264 Human)
UseTagsFlagExpressionMammalianMutationp.111LIDLY>AAAAA; c.331C>G,332T>C,334A&g…PromoterCMVAvailable sinceJune 12, 2024AvailabilityAcademic Institutions and Nonprofits only