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pJL1-mRFP1 gRNA

Plasmid

#117052

Purpose

In vitro expression of anti-mRFP1 Cas9 sgRNA from the T7 promoter

Depositor

Michael Jewett

Article

Stark et al ACS Synth Biol. 2019 May 7. doi: 10.1021

Insert

anti-mRFP1 SpCas9 gRNA

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

May 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-gp120(BaL)-CD4

Plasmid

#98957

Purpose

Mammalian expression plasmid for CD4-fused gp120 from HIV-1 BaL isolate

Depositor

Erik Procko

Article

Heredia et al J Immunol. 2018 Apr 20. pii: jimmunol.18

Insert

HIV-1 (BaL) gp120

Use

Tags

CD4 (domains D1-D2), CD5 leader sequence, FLAG ep…

Expression

Mammalian

Mutation

Codon-optimized synthetic gene

Promoter

CMV

Available since

April 24, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3.0_mitoLAMA-G97 IRES ShadowG-mScarlet

Plasmid

#130709

Purpose

Plasmid encoding outer mitochondrial localization of LAMA-G97 for GFP as a SNAP-Tag-fusion and ShadowG-mScarlet

Depositor

Kai Johnsson

Article

Farrants et al Nat Methods. 2020 Feb 17. pii: 10.1038/s

Insert

NTOM20-SNAP-Tag-LAMA-G97 IRES ShadowG-mScarlet

Use

Tags

NTOM20 and SNAP-Tag

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 26, 2019

Availability

Academic Institutions and Nonprofits only

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TXD13-CD4d3+4-bio

Plasmid

#73108

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding TXD13 with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

TXD13 (TMX4 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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bio-CD4d3+4-ADL1

Plasmid

#73090

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding ADL1 with bio-rat CD4d3+4

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

ADL1 (NCEH1 Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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MET7A-CD4d3+4-bio

Plasmid

#73127

Purpose

EXPRESs plasmid for human erythrocyte surface proteins encoding MET7A with rat CD4d3+4-bio

Depositor

Gavin Wright

Article

Crosnier et al Nature. 2011 Nov 9;480(7378):534-7. doi:

Insert

MET7A (METTL7A Human)

Use

Tags

biotinylation peptide and ratCD4d3+4

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 29, 2016

Availability

Academic Institutions and Nonprofits only

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pCIBN-EYFP

Plasmid

#103815

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Use

Tags

EYFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRSET-pr-mEos4b (mEosFP4b-V69T)

Plasmid

#99226

Purpose

Bacterial expression of the primed conversion capable protein pr-mEos4b (mEos4b-V69T).

Depositor

Periklis Pantazis

Article

Mohr et al Angew Chem Int Ed Engl. 2017 Jun 29. doi

Insert

pr-mEos4b

Use

Tags

Expression

Bacterial

Mutation

V69T for primed conversion applications

Promoter

T7

Available since

Oct. 23, 2017

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_1

Plasmid

#73533

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago1_2

Plasmid

#73534

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago1

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO1

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only
pGEX-5X1 Flag hDPY30

Plasmid

#15556

Depositor

Kai Ge

Article

Cho et al J Biol Chem. 2007 Jul 13. 282(28):20395-

Insert

DPY30 (DPY30 Human)

Use

Tags

Flag and GST

Expression

Bacterial

Mutation

Promoter

Available since

Aug. 16, 2007

Availability

Academic Institutions and Nonprofits only
Arp2_pLib

Plasmid

#173676

Purpose

Arp2 subunit of the Human Arp2/3 complex in a library vector for the biGBac system of insect expression

Depositor

Roberto Dominguez

Article

Zimmet et al Sci Adv. 2020 Jun 5;6(23). pii: 6/23/eaa

Insert

Arp2 (ACTR2 Human)

Use

Tags

Expression

Insect

Mutation

Promoter

polyhedrin

Available since

Sept. 1, 2021

Availability

Academic Institutions and Nonprofits only

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pCDNA5/FRT/TO_CEP41_HaloTag9

Plasmid

#169331

Purpose

Expression of HaloTag9 located at the microtubules of mammalian cells

Depositor

Kai Johnsson

Article

Frei et al Nat Methods. 2021 Dec 16. pii: 10.1038/s

Insert

CEP41-HaloTag9 (CEP41 Human)

Use

Tags

HaloTag9

Expression

Mammalian

Mutation

HaloTag9 = HaloTag7-Q165H-P174R

Promoter

Available since

Nov. 9, 2021

Availability

Academic Institutions and Nonprofits only

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pGEX Supt6h-SH2

Plasmid

#46518

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Supt6h (SUPT6H Human)

Use

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 1317-1441

Promoter

Tac

Available since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pLJM1 FRB-myc-RagA

Plasmid

#112740

Purpose

express FRB-myc-RagA

Depositor

Roberto Zoncu

Article

Lawrence et al Nat Cell Biol. 2018 Sep;20(9):1052-1063.

Insert

FRB-myc-RagA (RRAGA Human)

Use

Lentiviral

Tags

FRB, myc

Expression

Mutation

Promoter

Available since

Oct. 3, 2018

Availability

Academic Institutions and Nonprofits only

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pEY61

Plasmid

#191058

Purpose

acr-2(3.7k) fluorescent neural reporter driving nuclear mNeptune2.5 expression (refer to NeuroPAL paper for expression)

Depositor

Eviatar Yemini, Oliver Hobert

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

acr-2(3.7k) (acr-2 Nematode)

Use

Tags

Expression

Worm

Mutation

None

Promoter

Available since

Oct. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pBabe-PI(WT)-Rac1(WT)-mCherry

Plasmid

#87550

Purpose

PI(WT): Photo-inhibitable/blue light sensitive Rac1

Depositor

Klaus Hahn

Article

Dagliyan et al Science. 2016 Dec 16;354(6318):1441-1444

Inserts

LOV2
Rac1 (RAC1 Human)

Use

Tags

mCherry

Expression

Bacterial and Mammalian

Mutation

Promoter

CMV

Available since

April 27, 2017

Availability

Academic Institutions and Nonprofits only

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pBS NL4-3 envFS eGFP 5'CMV Δtat

Plasmid

#101347

Purpose

tat ATG->ACG (silent in vpr reading frame), nt 78 mutated T->G to change Tyr to stop codon, nt 116 mutated T->C to disrupt Met, 5'LTR replaced with CMV-R-U5 from pALPS for tat-independent transcription in HEK293E cells.

Depositor

Jeremy Luban

Article

McCauley et al Nat Commun. 2018 Dec 13;9(1):5305. doi:

Insert

NL4-3

Use

Lentiviral

Tags

Expression

Mutation

delta Tat

Promoter

Available since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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pCIBN-mCherry

Plasmid

#103816

Purpose

Soluble BLInCR construct that can interact with PHR constructs upon illumination with blue light

Depositor

Karsten Rippe

Article

Rademacher et al J Cell Sci. 2017 Dec 15;130(24):4213-422

Insert

CIBN (CIB1 Mustard Weed)

Use

Tags

mCherry

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pX458-sgRNA_Ago2_3

Plasmid

#73531

Purpose

Expresses SpCas9, GFP, and sgRNA targeting Ago2

Depositor

Constance Ciaudo

Article

Ngondo et al Stem Cell Reports. 2018 Jan 24. pii: S22

Insert

AGO2

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

hU6

Available since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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