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Plasmid#190069PurposeExpressing bioluminescent pH indicator-GeNL- in Arabidopsis thaliana apoplastDepositorInsertCel1 signal peptide-Green-enhanced Nano-lantern
UseTagsExpressionPlantMutationPromoterCaMV 35SAvailable sinceFeb. 9, 2023AvailabilityAcademic Institutions and Nonprofits only -
mKO2-SLBP(18-126)
Plasmid#83914PurposeFluorescent probe for S/G2 cell cycle transitionDepositorInsertmKO2-SLBP(18-126) (SLBP Synthetic, Human)
UseLentiviralTagsExpressionMammalianMutationPromoterCMVAvailable sinceNov. 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pALOD4 non-binding mutant
Plasmid#111027PurposeExpresses non-binding mutant version of p-ALOD4, has six inactivating mutations (G501A, T502A, T503A, L504A, Y505A, and P506A)DepositorInsertDomain 4 of Anthrolysin O
UseTagsHis6 tagExpressionBacterialMutationMutations: S404C, C472A, G501A, T502A, T503A, L5…PromoterT7Available sinceJune 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP3 CKK
Plasmid#59033Purposeexpresses CKK domain of CAMSAP3 in E. coliDepositorInsertCAMSAP3 CKK domain (CAMSAP3 Human)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceSept. 2, 2014AvailabilityAcademic Institutions and Nonprofits only -
pCEP4-CCR5
Plasmid#98945PurposeMammalian expression plasmid for untagged human CCR5DepositorInsertCCR5 (CCR5 Human)
UseTagsExpressionMammalianMutationFull-length, wildtypePromoterCMVAvailable sinceApril 24, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1-Hyg-RFPKDEL
Plasmid#138660PurposeReporter plasmid expressing the ER-located RFPDepositorInsertRFP-KDEL
UseTagsKDELExpressionMammalianMutationPromoterCMVAvailable sinceMarch 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
pFAB815
Plasmid#47854PurposeBIOFAB reporter plasmid for measuring rnpB T1 termination efficiencyDepositorInsertrnpB T1 terminator
UseSynthetic BiologyTagsExpressionBacterialMutationPromoterpLTetO9Available sinceDec. 3, 2013AvailabilityAcademic Institutions and Nonprofits only -
pC029 - Lw2Cas13a from Leptotrichia wadei F0279
Plasmid#91919PurposeExpresses Lw2Cas13a for bacterial expression and contains backbone for spacer cloningDepositorInsertLw2Cas13a and guide expression cassette
UseCRISPRTagsExpressionBacterialMutationPromoterAvailable sinceOct. 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
MSP2262
Plasmid#70703PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 1: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #1DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A & H557A in SaCas9PromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-eDHFR-MCS
Plasmid#172108PurposeMammalian expression of a protein of interest fused to the C-terminus of EGFP-eDHFRDepositorInsertEGFP-eDHFR-20aa (folA E. coli)
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceAug. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCFP-LacI-NLS
Plasmid#103840PurposeFlourescent marker for lacO arraysDepositorInsertCFP-LacI-NLS
UseTagsExpressionMammalianMutationLacI: C19T (silent, L7L), T264C (silent, A88A), C…PromoterSV2Available sinceJan. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP2 CKK
Plasmid#59032Purposeexpresses CKK domain of CAMSAP2 in E. coliDepositorInsertCAMSAP2 CKK domain (CAMSAP2 Human)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
pET-28a+GFP-CAMSAP1 CKK
Plasmid#59031Purposeexpresses CKK domain of CAMSAP1 in E. coliDepositorInsertCAMSAP1 CKK domain (CAMSAP1 Human)
UseTagsHis, Strep, TEV, and sfGFPExpressionBacterialMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
MSP2279
Plasmid#70706PurposeBacterial expression plasmid for Sa-dCas9 (D10A/H557A) & sgRNA targeted to site 2: T7-humanSadCas9-NLS-3xFLAG-T7-Sa-sgRNA(84) #2DepositorInsertsmammalian codon-optimized Staphylococcus aureus dCas9
SaCas9 sgRNA (+84)
UseCRISPRTagsNLS-3xFLAGExpressionBacterialMutationD10A and H557A in SaCas9PromoterT7Available sinceDec. 4, 2015AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2840 pPB: CAG-Puro-WPRE PGK-VPR-tagBFP-SadCas9
Plasmid#84246PurposeExpresses direct fusion VPR-Sa dCas9DepositorInsertsVPR-tagBFP-Sa dCas9
Puro
UseCRISPR and Synthetic Biology; PiggybacTagsNucleoplasmin NLS, SV40 NLS, VPR, and tagBFPExpressionMammalianMutationPromoterCAG and PGKAvailable sinceNov. 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCI pHluorin Syt11
Plasmid#184512PurposeMammalian expression of Synaptotagmin 11 with pH-sensitive fluorescent proteinDepositorInsertSynaptotagmin 11 (Syt11 Mouse)
UseTagsSuper Ecliptic pHluorinExpressionMammalianMutationPromoterAvailable sinceMay 15, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ2804 pHR: U6-SpsgTRE3G CMV-PYL1-VPR-IRES-mCherry
Plasmid#84258PurposeExpresses Sp sgTRE3G gRNA with ABA-inducible VPR and mCherry for OR gateDepositorInsertsSp sgTRE3G
PYL1-VPR
UseCRISPR and LentiviralTagsIRES-mCherry and PYL1ExpressionMammalianMutationPromoterCMV and mouse U6Available sinceNov. 28, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFasBac+GFP-CAMSAP3 CC+CKK
Plasmid#59041Purposefor baculovirus expression of mouse CAMSAP3 CC+CKK in Sf9 cellsDepositorInsertCAMSAP3 CC+CKK domain (Camsap3 Mouse)
UseTagsHis and eGFPExpressionInsectMutationPromoterAvailable sinceAug. 27, 2014AvailabilityAcademic Institutions and Nonprofits only -
IBMc071
Plasmid#161943PurposeContains Type IIS restriction site free elements for expressing protein to be split in IBM. ECF20 expression plasmidDepositorInsertpSB3T5(BsaI-)-PBAD(SapI-)-B33-ECF20-T
UseTagsExpressionBacterialMutationPromoterPBADAvailable sinceMarch 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pRC/CMV-TYK2P1104A-VSV
Plasmid#139350PurposeExpression of the TYK2-P1104A variant, rs34536443DepositorInsertTYK2 cDNA with 267nt of 5' UTR (TYK2 Human)
UseTagsVSVExpressionMammalianMutationV362F-P1104APromoterCMVAvailable sinceMarch 24, 2020AvailabilityAcademic Institutions and Nonprofits only