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FUGW-Cytosol-TurboID

Plasmid

#200961

Purpose

3rd gen lentiviral plasmid for expressing cytosol localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Cytosol-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pHAGE-EF1aL-eGFP-W

Plasmid

#126686

Purpose

Constitutive, ubiquitous GFP expression.

Depositor

Darrell Kotton

Article

Jacob et al Nat Protoc. 2019 Dec;14(12):3303-3332. d

Insert

eGFP

Use

Lentiviral

Tags

Expression

Mutation

Promoter

EF1aL

Available since

June 26, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)_SpyCatcher-6aa-sfCherry2(1-10)

Plasmid

#117484

Purpose

Expresses tandem binder (SpyCatcher002-6aa-sfCherry2(1-10)) in mammalian cells

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Insert

SpyCatcher002-6aa-sfCherry2(1-10)

Use

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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EGFP-Rab11a-7

Plasmid

#56444

Purpose

Localization: Ras GTPase, Excitation: 488, Emission: 507

Depositor

Michael Davidson

Article

Fang et al Traffic. 2010 Jun;11(6):782-99. doi: 10.

Insert

Rab11a (RAB11A Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 13, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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FUGW-Golgi-TurboID

Plasmid

#200964

Purpose

3rd gen lentiviral plasmid for expressing golgi localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Golgi-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pDP085

Plasmid

#198425

Purpose

pUBQ10:BirA. A pGreen based plasmid expressing E. coli BirA after the Arabidopsis pUBQ10 promoter. This plasmid is originated from You et al., Nat Commun. 2017, doi: 10.1038/ncomms15120

Depositor

Markus Schmid

Article

You et al Nat Commun. 2017 May 17;8:15120. doi: 10

Insert

E. coli BirA

Use

Tags

Expression

Plant

Mutation

Promoter

pUBQ10

Available since

March 22, 2023

Availability

Academic Institutions and Nonprofits only

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Mito-TurboID

Plasmid

#200960

Purpose

Transiently expresses mitochondria localized TurboID with V5 tags

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

mito-TurboID

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pETDuet_sfCherry2(1-10)_sfCherry2(11)-SpyCatcher

Plasmid

#117656

Purpose

Bacterial expression vector for split sfCherry3 screening. The “sfCherry2(1-10)” and “sfCherry2(11)-SpyCatcher002” are expressed from two different T7 promoters.

Depositor

Bo Huang

Article

Feng et al Commun Biol. 2019 Sep 17;2:344. doi: 10.

Inserts

sfCherry2(1-10)
sfCherry2(11)-SpyCatcher002

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 29, 2019

Availability

Academic Institutions and Nonprofits only

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Lenti_SaCRISPR_GFP

Plasmid

#118636

Purpose

To clone sgRNA for SaCas9 (SauCas9)

Depositor

Christopher Vakoc

Article

Lu et al Cancer Cell. 2018 Dec 10;34(6):970-981.e

Type

Empty backbone

Use

Lentiviral

Tags

Expression

Mutation

Promoter

U6

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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LentiV_Neo_SIK3

Plasmid

#108103

Purpose

Lentiviral expression plasmid of human SIK3 cDNA with neomycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

SIK3 (SIK3 Human)

Use

CRISPR and Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

EFS promoter

Available since

April 5, 2018

Availability

Academic Institutions and Nonprofits only

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FUGW-ER-TurboID

Plasmid

#200965

Purpose

3rd gen lentiviral plasmid for expressing endoplasmic reticulum localized TurboID with EGFP

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

ER-TurboID

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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HBT-pcoCas9

Plasmid

#52254

Purpose

transient expression of pcoCas9 gene in plant cells

Depositor

Jen Sheen

Article

Li et al Nat Biotechnol. 2013 Aug;31(8):688-91. d

Insert

pcoCas9

Use

CRISPR; Plant expression

Tags

FLAG and NLS

Expression

Mutation

Promoter

hybrid constitutive promoter 35SPPDK

Available since

March 20, 2014

Availability

Academic Institutions and Nonprofits only

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Golgi-TurboID

Plasmid

#200959

Purpose

Transiently expresses golgi localized TurboID with HA tags

Depositor

Keriann Backus

Article

Yan et al Cell Chem Biol. 2023 Jul 20;30(7):811-82

Insert

Golgi-TurboID

Use

Tags

HA

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Aug. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pECE-M2-PAK2 wt

Plasmid

#31663

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

p21-activated protein kinase 2 (PAK2 Human)

Use

Tags

M2

Expression

Mammalian

Mutation

Promoter

Available since

April 11, 2012

Availability

Academic Institutions and Nonprofits only

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mNeonGreen-α-synuclein

Plasmid

#205713

Purpose

Mammalian expression of mNeonGreen-alpha-synuclein

Depositor

Randy Schekman

Article

Wu et al Elife. 2023 Jan 10;12:e85837. doi: 10.75

Insert

alpha-synuclein (SNCA Human)

Use

Tags

mNeonGreen

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 26, 2024

Availability

Academic Institutions and Nonprofits only

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TRAC_DARIC(2-3)_CD22-BBz_AAV6_Donor

Plasmid

#211906

Purpose

Vector for AAV6 donor production. Targeted CD22-BBz DARIC transgene integration to the TRAC locus via homology-directed repair.

Depositor

Yannick Doyon

Article

Levesque et al bioRxiv 2023.09.14.557485

Insert

DARIC-CD22-BBz

Use

AAV

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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PCMV-intron myc Rab1aWT

Plasmid

#46776

Purpose

expression of WT Rab1a in mammalian cells

Depositor

Terry Hébert

Article

Dupre et al J Biol Chem. 2006 Nov 10;281(45):34561-7

Insert

Rab1a (RAB1A Human)

Use

Tags

myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 14, 2013

Availability

Academic Institutions and Nonprofits only

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pYES1L-URA

Plasmid

#84301

Purpose

E.coli shuttle vector for large-scale DNA assembly in S. cerevisiae.

Depositor

George Church

Article

Ostrov et al Science. 2016 Aug 19;353(6301):819-22. d

Type

Empty backbone

Use

Tags

Expression

Bacterial and Yeast

Mutation

Promoter

Available since

May 17, 2018

Availability

Academic Institutions and Nonprofits only

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pBA407

Plasmid

#85970

Purpose

UPRE-mCh-Ubc-Neo

Depositor

Jonathan Weissman

Article

Adamson et al Cell. 2016 Dec 15;167(7):1867-1882.e21.

Inserts

mCherry
Neo

Use

Lentiviral

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Feb. 1, 2017

Availability

Academic Institutions and Nonprofits only

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pDest-303

Plasmid

#159678

Purpose

Gateway destination vector for mammalian protein expression

Depositor

Dominic Esposito

Article

Goswami et al Elife. 2020 Jan 20;9. pii: 47654. doi: 1

Type

Empty backbone

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 5, 2020

Availability

Academic Institutions and Nonprofits only

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