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Plasmid#59018PurposeExpresses a fusion of yellow fluorescent protein (YFP) to the N-terminus of the Escherichia coli Tn10 tet repressor (TetR) driven by the alpha tubulin promoter for use in T. gondiiDepositorInsertYFP-TetR
UseToxoplasma expressionTagsExpressionMutationPromoteralpha-tubulinAvailable sinceSept. 29, 2014AvailabilityAcademic Institutions and Nonprofits only -
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pYNL-N1_TUBB5
Plasmid#65678PurposeExpresses beta-tubulin-YNL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsYNLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
pRvTUB-mEGFP-NLS
Plasmid#205011PurposeThe vector contains 1kbp of the upstream and downstream regions of the tubulin gene from Ramazzottius varieornatus, with the mEGFP gene with NLS sequence positioned in between.DepositorInsertmEGFP-NLS flanked by 1kbp upstream and downstream of the tubulin gene from R. varieornatus
UseTardigrade expressionTagsExpressionMutationPromoterAvailable sinceAug. 22, 2023AvailabilityAcademic Institutions and Nonprofits only -
pIW086
Plasmid#134132PurposeBacterial expression of His-TEV-Ska1 and Ska2DepositorInsertHis-TEV-human Ska1 ; human Ska2
UseTagsHis-TEVExpressionBacterialMutationK183A K184APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDNA3 humanN-Flag SadBS
Plasmid#66881PurposeMammalian expression vector for expressing N-terminally Flag-tagged short isoform of SadBDepositorInsertSadB (BRSK1 Human)
UseTagsFlagExpressionMammalianMutationPromoterCMVAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
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pGex2t SadBs
Plasmid#66882PurposeBacterial expression vector encoding N-terminally GST-tagged short isoform of human SadBDepositorInsertSadB (BRSK1 Human)
UseTagsGSTExpressionBacterialMutationPromoterAvailable sinceJune 25, 2015AvailabilityAcademic Institutions and Nonprofits only -
pONL-N1_TUBB5
Plasmid#65680PurposeExpresses beta-tubulin-ONL in mammalian cellsDepositorInsertbeta-tubulin class V
UseLuciferaseTagsONLExpressionMammalianMutationPromoterCMVAvailable sinceJune 18, 2015AvailabilityAcademic Institutions and Nonprofits only -
1114H
Plasmid#200640PurposeAn. gambiae transgenesis plasmid. Expresses gRNAs targeting B2-tubulin, ZPG, and DSXF. Crossing to Cas9 generates sterile malesDepositorInsertgRNA targeting DSXF, ZPG, and B2-tubulin. Actin5c-CFP and Vasa2-EYFP reporters.
UseCRISPRTagsExpressionInsectMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pDK616
Plasmid#134202PurposeBacterial expression of human Ska1 microtubule binding domain (MTBD) mutantDepositorInsertHis-Precission-human Ska1 MTBD (SKA1 Human)
UseTagsExpressionBacterialMutationK223A K226APromoterAvailable sinceFeb. 18, 2020AvailabilityAcademic Institutions and Nonprofits only -
pJS263
Plasmid#134141PurposeBacterial expression of C. elegans Ska1 microtubule binding domain (MTBD)DepositorInsertSka1 MTBD-His (Y106G6H.15 Nematode)
UseTagsExpressionBacterialMutationPromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW087
Plasmid#134128PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationK183A K184APromoterAvailable sinceFeb. 7, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW090
Plasmid#134129PurposeBacterial expression of GFP-Ska1 and Ska2DepositorInsertHis-GFP-human Ska1 ; human Ska2
UseTagsGFPExpressionBacterialMutationK223A K226APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW070
Plasmid#134138PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 delta MTBD CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationresidues 1-131, CRISPR resistantPromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW093
Plasmid#134137PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, R155APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW082
Plasmid#134136PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, R236APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW088
Plasmid#134135PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, K223A K226APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIW085
Plasmid#134134PurposeRetroviral expression of GFP-Ska1DepositorInsertGFP-human Ska1 CRISPR/Cas9 hardened (SKA1 Human)
UseRetroviralTagsGFPExpressionMammalianMutationCRISPR resistant, K183A K184APromoterAvailable sinceJan. 30, 2020AvailabilityAcademic Institutions and Nonprofits only