-
Plasmid#223878Purpose4G-cloning donor for N-terminal His6-GST-TEV tagDepositorInsertHis6-GST-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-MBP-TEV
Plasmid#223877Purpose4G-cloning donor for N-terminal His6-MBP-TEV tagDepositorInsertHis6-MBP-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-HA2-TEV
Plasmid#223876Purpose4G-cloning donor for N-terminal His6-HA2-TEV tagDepositorInsertHis6-HA2-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pD(Nt)_His6-FLAG3-TEV
Plasmid#223875Purpose4G-cloning donor for N-terminal His6-FLAG3-TEV tagDepositorInsertHis6-FLAG3-TEV
UseGolden-gate donorTagsExpressionMutationPromoterAvailable sinceSept. 19, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM-TEV
Plasmid#222101PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.DepositorInsertBacteroidetes-bGSDM-TEV ORF
UseTags6xHis-SUMO2ExpressionBacterialMutationTEV protease site replaces residues E242–G248PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pNIC-GST-TEV-GG
Plasmid#215795PurposeCloning of target gene with N terminal GST tag and TEV cleavageDepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceApril 25, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pET28b-TEV-AcrIC9
Plasmid#207409PurposeProtein purification vector to produce AcrIC9 in E. coliDepositorInsertT7-TEV-AcrIC9
UseTagsHisExpressionBacterialMutationPromoterT7Available sinceFeb. 16, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-AncSZ
Plasmid#214235PurposeBacterial expression construct of AncSZ, an kinase domain engineered through ancestorial reconstruction of the kinase domains of both SYK and ZAP-70. For in vitro protein tyrosine phosphorylationDepositorInsertSyk kinase (SYK Human)
UseTags6xHisExpressionBacterialMutationSequence gained through ancestral reconstruction …PromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cAbl
Plasmid#214234PurposeBacterial expression of the kinase domain of cAbl with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertABL1 Kinase domain (ABL1 Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET23a-H6-TEV-cSrc
Plasmid#214233PurposeBacterial expression of the kinase domain of cSrc with a TEV-cleavable N-terminal 6xHis affinity tag; For in vitro tyrosine phosphorylation in peptides/proteins and for kinase specificity screeningsDepositorInsertcSrc Kinase domain (SRC Human)
UseTags6xHisExpressionBacterialMutationPromoterT7Available sinceFeb. 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
Ca-LiPPI-CLAPon1.0 (TEVp)
Plasmid#213409PurposeLight-gated peroxidase (APEX) reporter for detecting calcium-dependent CaM and MK2 interaction (component 2)DepositorInsertCaM-V5-TEVp
UseLentiviralTagsV5ExpressionMammalianMutationPromoterCMVAvailable sinceFeb. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
Annexin A7-TEV-TwinStrep
Plasmid#198635PurposeAnnexin A7 fibrillization and lipid bindingDepositorInsertAnnexin A7 (ANXA7 Human)
UseTagsTEV-GS-Dual StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-C-TEV-HAT
Plasmid#202597PurposeExpress N-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pCAGGS-N-TEV-HAT
Plasmid#202598PurposeExpress C-terminal Tobacco Etch Virus (TEV) protease cleavable Histidine Affinity Tag (HAT) (KDHLIHNVHKEEHAHAHNK) in mammalian cellDepositorTypeEmpty backboneUseTagsTEV cleavable site (ENLYFQG) and poly-histidine a…ExpressionMammalianMutationPromoterCAGAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(304-393)
Plasmid#200313Purposebacterial expression of p53 TET-CTDDepositorInsertp53(304-393) (TP53 Human)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceJune 23, 2023AvailabilityAcademic Institutions and Nonprofits only -
H6GB1tevP53(363-393)
Plasmid#200314Purposebacterial expression of P53 CTDDepositorInsertp53(363-393) (TP53 Human)
UseTagsHis6-GB1-tevExpressionBacterialMutationPromoterT7Available sinceMay 17, 2023AvailabilityAcademic Institutions and Nonprofits only -
pnCS_SEPT7_SEPT9_i1-i5-TEV-Strep
Plasmid#180314Purposebacterial co-expression of human SEPT7 and of human SEPT9_i1-i5DepositorUseTagsTEV-StrepExpressionBacterialMutationSEPT9_i1-i5PromoterAvailable sinceMarch 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pnCS_DSep2_Peanut-TEV-Strep
Plasmid#174495Purposebacterial co-expression of Drosophila Sep2 and of Drosophila PeanutDepositorUseTagsTEV-StrepExpressionBacterialMutationPromoterAvailable sinceSept. 29, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-uTEV1-SQSfl
Plasmid#171010PurposeHiLITR protease with full-length SQS targeting information (ER)DepositorInsertEGFP-uTEV1-SQS(FL)
UseLentiviral and Synthetic BiologyTagsExpressionMammalianMutationPromoterpTRE-tight (TetOn)Available sinceAug. 19, 2021AvailabilityAcademic Institutions and Nonprofits only -
WIPI2d-TEVcs-TSF
Plasmid#171419PurposeFor the purifcation of WIPI2d proteinDepositorInsertWIPI2d (WIPI2 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 13, 2021AvailabilityAcademic Institutions and Nonprofits only