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Plasmid#127552PurposeVariant of pKD13. Lambda Red recombineering PCR template plasmid carrying an F5-tetA-F5 cassette orthogonal to FRT-KanR-FRT in pKD13DepositorInsertF5-flanked tetracycline resistance cassette
UseTagsExpressionBacterialMutationMultiple SNPs in tetA (originally from pBR322) to…PromoterAvailable sinceJuly 30, 2019AvailabilityAcademic Institutions and Nonprofits only -
pCI152-pLenti-SiT-Cas12a
Plasmid#128405PurposeExpress both AsCas12a and CRISPR arrays on single transcriptsDepositorInsertAsCas12a-Triplex
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceOct. 1, 2019AvailabilityAcademic Institutions and Nonprofits only -
pFS_0235-pET30-Fusicatenibacter_saccharivorans_ARRAY2_FaqI
Plasmid#116954PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP2 MCR-1
Plasmid#118404PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the pLac promoter.DepositorInsertMCR-1
UseTagsExpressionBacterialMutationPromoterpLacAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0351_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1
Plasmid#116963PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0353_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2
Plasmid#116965PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0352_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_1_RC
Plasmid#116964PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 5, 2018AvailabilityAcademic Institutions and Nonprofits only -
pDD379
Plasmid#91834PurposeSapTrap destination vector for building combined sgRNA expression + repair template vectors, using the F+E sgRNA scaffoldDepositorTypeEmpty backboneUseCRISPRTagsExpressionWormMutationPromoterAvailable sinceJune 23, 2017AvailabilityAcademic Institutions and Nonprofits only -
pFS_0143-pET30-Fusicatenibacter_saccharivorans
Plasmid#116952PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 1, compatible with classic acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0354_pET30-RT-CRISPR_Fusicatenibacter_saccharivorans_ARRAY_2_RC
Plasmid#116966PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2 RC, compatible with SENECA acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0160-pET30-Fusicatenibacter_saccharivorans_ARRAY2
Plasmid#116953PurposeExpresses FsRT-Cas1-Cas2 under pT7lac promoter, encodes FsCRISPR array 2, compatible with classic acquisition readoutDepositorInsertFusicatenibacter saccharivorans RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 2, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP3 arr
Plasmid#112890PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertarr
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 fosA
Plasmid#112889PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertfosA
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0385_pET30-RT-CRISPR_Rivularia_sp._PCC_7116_ARRAY_1
Plasmid#116997PurposeExpresses RPCC7116RT-Cas1-Cas2 under pT7lac promoter, encodes RPCC7116CRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertRivularia sp. PCC 7116 RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGDP1 stat
Plasmid#112886PurposeThis plasmid is part of the Minimal Antibiotic Resistance Platform (ARP) Kit (Addgene #1000000143). Low copy-number plasmid that constitutively expresses genes using the Pbla promoter.DepositorInsertstat
UseTags6xHisExpressionBacterialMutationPromoterPblaAvailable sinceDec. 10, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0376_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_1_RC
Plasmid#116988PurposeExpresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertTolypothrix campylonemoides RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0357_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1
Plasmid#116969PurposeExpresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertMicromonospora rosaria RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0358_pET30-RT-CRISPR_Micromonospora_rosaria_ARRAY_1_RC
Plasmid#116970PurposeExpresses MrRT-Cas1-Cas2 under pT7lac promoter, encodes MrCRISPR array 1 RC, compatible with SENECA acquisition readoutDepositorInsertMicromonospora rosaria RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0369_pET30-RT-CRISPR_Azospirillum_lipoferum_4B_ARRAY_1
Plasmid#116981PurposeExpresses AlRT-Cas1-Cas2 under pT7lac promoter, encodes AlCRISPR array 1, compatible with SENECA acquisition readoutDepositorInsertAzospirillum lipoferum 4B RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only -
pFS_0380_pET30-RT-CRISPR_Tolypothrix_campylonemoides_ARRAY_3_RC
Plasmid#116992PurposeExpresses TcRT-Cas1-Cas2 under pT7lac promoter, encodes TcCRISPR array 3 RC, compatible with SENECA acquisition readoutDepositorInsertTolypothrix campylonemoides RT-Cas1-Cas2
UseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 29, 2018AvailabilityAcademic Institutions and Nonprofits only