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We narrowed to 9,196 results for: plasmids 101

Showing: 341 - 360 of 9196 results

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AiP14283 - pAAV-AiE1014h_3xC3-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN4283)

Plasmid

#230394

Purpose

AiE1014h_3xC3 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Jonathan Ting

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 24, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13470 - pAAV-AiE1014h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3470)

Plasmid

#230152

Purpose

AiE1014h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AiP13473 - pAAV-AiE1017h-minBG-SYFP2-P2A-3XFLAG-10aa-H2B-WPRE3-BGHpA (Alias: CN3473)

Plasmid

#230153

Purpose

AiE1017h is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

Depositor

The Allen Institute for Brain Science, Boaz Levi

Article

Ben-Simon et al bioRxiv [Preprint]. 2024 Sep 26:2024.06.

Insert

SYFP2-P2A-3XFLAG-10aa-H2B

Use

AAV

Tags

Expression

Mutation

Promoter

minBG

Available since

Feb. 20, 2025

Availability

Academic Institutions and Nonprofits only

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AAV sgRNA Expression Plasmid

Plasmid

#174540

Purpose

Contains restriction sites to clone in U6 promoter and sgRNA oligo. CMV-driven mCherry fluorescent marker.

Depositor

Ormond MacDougald

Article

Romanelli et al J Biol Chem. 2021 Nov 11:101402. doi: 10

Type

Empty backbone

Use

AAV

Tags

None

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Nov. 16, 2021

Availability

Academic Institutions and Nonprofits only

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Noodles Plasmid

Plasmid

#212613

Purpose

An all-in-one system for on-target efficiency analysis of CRISPR guide RNAs

Depositor

Ilya Vinnikov

Article

Lin et al MethodsX. 2023 Nov 15;12:102481. doi: 10

Type

Empty backbone

Use

CRISPR and Luciferase

Tags

Expression

Mammalian

Mutation

Promoter

Available since

April 9, 2024

Availability

Academic Institutions and Nonprofits only

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Plasmid EUPp_09

Plasmid

#157745

Purpose

PgyrA_ada _adh2 _pMB1_ampR (for expressing two enzymes (Ada and Adh2) to allow E. coli to consume ethanol)

Depositor

Kang Zhou

Article

Liang et al Metab Eng. 2020 Nov 25. pii: S1096-7176(

Inserts

acetaldehyde dehydrogenase
alcohol dehydrogenase (ADH2 Budding Yeast)

Use

Tags

Expression

Bacterial

Mutation

Promoter

PgyrA

Available since

June 4, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-IL2sp-6His-Asprosin plasmid

Plasmid

#190683

Purpose

Expresses His-tagged asprosin in mammalian cells. An IL2 signal peptide at N-terminus allows secretion of asprosin from the transduced cells.

Depositor

Atul Chopra

Article

Mishra et al STAR Protoc. 2022 Dec 16;3(4):101762. do

Insert

IL2-6xHis-Asprosin

Use

Tags

IL2 signal peptide, 6xHis

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 8, 2022

Availability

Academic Institutions and Nonprofits only

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eIF3k sgRNA-2 PX459 plasmid

Plasmid

#192256

Purpose

expressing sgRNA-2 targeting eIF3k locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-2 targeting eIF3k locus closed to stop coden (EIF3K Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 6, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k sgRNA-1 PX459 plasmid

Plasmid

#192255

Purpose

expressing sgRNA-1 targeting eIF3k locus closed to stop coden

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

sgRNA-1 targeting eIF3k locus closed to stop coden (EIF3K Human)

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pCut plasmid toolkit

Plasmid Kit

#1000000118

Purpose

Cas9-based kit in S. cerevisiae for optimizing heterologous gene expression. Cas9-sgRNA (pCut) plasmids for integrating into 23 loci, across all 16 yeast chromosomes.

Depositor

Aindrila Mukhopadhyay

Available since

Aug. 4, 2017

Availability

Academic Institutions and Nonprofits only
eIF3k- pMK289 (mAID-mClover-NeoR) plasmid

Plasmid

#192243

Purpose

knockin donor vector of mAID-mClover-NeoR to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mClover-NeoR (EIF3K Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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eIF3k- pMK293 (mAID-mCherry2-Hygro) plasmid

Plasmid

#192244

Purpose

knockin donor vector of mAID-mCherry2-Hygro to C-terminus of endogenous human eIF3k

Depositor

Dieter Wolf

Article

Duan et al EMBO J. 2023 Jun 15;42(12):e112362. doi:

Insert

eIF3k-knockin-homology arm-mAID-mCherry2-Hygro (EIF3K Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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PAX6 donor plasmid

Plasmid

#105239

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Article

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette

Use

TALEN

Tags

Expression

Mutation

Promoter

Available since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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Tn7 helper plasmid

Plasmid

#141161

Purpose

Temperature-sensitive plasmid carries tnsABCD genes that encode transposase biochemical machinery under an arabinose-inducible pBAD promoter

Depositor

Shimon Bershtein

Article

Jasinska et al Nat Ecol Evol. 2020 Feb 24. pii: 10.1038

Insert

Tn7 transposon machinery

Use

Tags

Expression

Bacterial

Mutation

Promoter

Arabinose-inducible pBAD

Available since

Aug. 11, 2020

Availability

Academic Institutions and Nonprofits only

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BRD4 CRISPRi plasmid

Plasmid

#154890

Purpose

Human BRD4 CRISPRi gRNA

Depositor

Joseph Dougherty, Rob Mitra

Article

Moudgil et al Cell. 2020 Aug 20;182(4):992-1008.e21. d

Insert

BRD4-targeting gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

July 17, 2020

Availability

Academic Institutions and Nonprofits only

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CJ8421_04975 Rptr2 recording plasmid

Plasmid

#194211

Purpose

CJ8421_04975 Rptr2 targeted DNA sequence

Depositor

Chase Beisel

Article

Jiao et al Nat Biotechnol. 2023 Jan 5. doi: 10.1038

Insert

CJ8421_04975 Rptr2 DNA target

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

May 23, 2023

Availability

Academic Institutions and Nonprofits only

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Non-targeting CRISPRi plasmid

Plasmid

#154891

Purpose

Human non-targeting CRISPRi gRNA

Depositor

Joseph Dougherty, Rob Mitra

Article

Lalli et al Genome Res. 2020 Sep;30(9):1317-1331. do

Insert

Nontargeting gRNA

Use

CRISPR and Lentiviral

Tags

Expression

Mutation

Promoter

Available since

Nov. 18, 2020

Availability

Academic Institutions and Nonprofits only

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PAX6 CKO 2/7 Donor plasmid

Plasmid

#68467

Purpose

donor plasmid for targeting PAX6 locus

Depositor

Su-Chun Zhang

Article

Chen et al Cell Stem Cell. 2015 Jul 1. pii: S1934-5

Insert

FRT flanked exon4 of PAX6 gene (PAX6 Human)

Use

Cre/Lox

Tags

Expression

Mammalian

Mutation

Promoter

mouse PGK promoter

Available since

Nov. 25, 2015

Availability

Academic Institutions and Nonprofits only

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DTECT - Plasmid for Standard Curve

Plasmid

#139333

Purpose

This plasmid is used to generate the unique standard curve required to quantify the capture of genomic signature using DTECT

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

DTECT ligation product

Use

Unspecified

Tags

Expression

Mutation

Promoter

Available since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pU6-hL1-5'_3.3kb plasmid

Plasmid

#226006

Purpose

Plasmid used to generate DNA-FISH probe targeting at human L1HS

Depositor

Miguel Ramalho-Santos

Article

Zhang et al Dev Cell. 2024 Oct 14:S1534-5807(24)0057

Insert

Human L1 element 3.3kb sequences at the 5' end

Use

Synthetic Biology

Tags

Expression

Mutation

Promoter

Available since

Oct. 16, 2024

Availability

Academic Institutions and Nonprofits only

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