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Plasmid#79835PurposeExpresses PpsR2-mVenus-CAAX under CMVd1 promoterDepositorInsertPpsR2-mVenus-CAAX
UseTagsExpressionMammalianMutationRpPpsR2 cysteine 439 changed to serinePromoterCMVd1Available sinceAug. 22, 2016AvailabilityAcademic Institutions and Nonprofits only -
pCMV-160
Plasmid#79840PurposeExpresses NLS-PpsR2-mVenus under CMV promoterDepositorInsertNLS-PpsR2-mVenus
UseTagsExpressionMammalianMutationRpPpsR2 cysteine 439 changed to serinePromoterCMVAvailable sinceJuly 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmVen-RPA70-IRES-RPA32-IRES-RPA14
Plasmid#211431Purposemammalian expression of human RPA70 N-terminally fused to mVenus and RPA32 and RPA14DepositorUseTagsmVenus (on RPA70)ExpressionMammalianMutationPromoterAvailable sinceJan. 26, 2024AvailabilityAcademic Institutions and Nonprofits only -
pYFP
Plasmid#175592PurposeTet inducible, KAN Resistant, mVenus gene on a low copy backbone. Used as a negative control to the pRelA_YFP plasmid.DepositorInsertmVenus
UseTagsExpressionBacterialMutationAn improved monomeric YFP. See doi: 10.1021/bi051…PromoterAvailable sinceNov. 23, 2021AvailabilityAcademic Institutions and Nonprofits only -
Dimer 42 AA
Plasmid#198198PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 42AA linkerDepositorInsertmVenus-42AA-mTurquoise2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
Dimer 3 AA
Plasmid#198197PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 3AA linkerDepositorInsertmVenus-3AA-mTurquoise2
UseTagsExpressionMammalianMutationPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pRPA70-IRES-Ven-RPA32-IRES-RPA14
Plasmid#211430Purposemammalian expression of human RPA32 N-terminally fused to mVenus and RPA70 and RPA14DepositorUseTagsmVenus (on RPA32)ExpressionMammalianMutationPromoterAvailable sinceJan. 29, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCaggs-mGold
Plasmid#157996PurposeExpression of mGold in mammalian cellsDepositorInsertmGold
UseTagsExpressionMammalianMutationmGold is mVenus with L46F;T63S mutationsPromoterActin promoter with CMV enhancerAvailable sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCAG-NCad-V
Plasmid#191665PurposemVenus-inserted mouse N-cadherinDepositorInsertN-cadherin
UseTagsHA and mVenusExpressionMammalianMutationPromoterChicken β-actin promoterAvailable sinceOct. 24, 2022AvailabilityAcademic Institutions and Nonprofits only -
pJK506
Plasmid#155382PurposedCas12a (D917A) + PA4-mVenus. (removed crRNAs)DepositorInsertsdCas12a (F. novicida)
PA4-mVenus
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceJan. 14, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJL-mGold
Plasmid#157997PurposeExpression of mGold in yeast cellsDepositorInsertmGold
UseTagsExpressionYeastMutationmGold is mVenus with L46F;T63S mutationsPromoterpTDH(GAP promoter)Available sinceOct. 26, 2020AvailabilityAcademic Institutions and Nonprofits only -
Dimer cleaved
Plasmid#198199PurposeFRET calibration: Dimer construct consisting of mTurquoise2 and mVenus with a 2A peptide linker for posttranslational linker cleavageDepositorInsertmVenus-T2A-mTurquoise2
UseTagsExpressionMammalianMutationA206K bothPromoterAvailable sinceJuly 5, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJK384.4
Plasmid#155369PurposedCas9 + PA4-mVenusDepositorInsertsdCas9 (bacteria)
PA4-mVenus
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pJK412
Plasmid#155371PurposedCas9 oscillator v1 (dCRv1) + [dCas9 + PA4-mVenus]DepositorInsertsdCas9 (bacteria)
PA4-mVenus
dCas9 sgRNA oscillator v1
UseCRISPRTagsExpressionBacterialMutationD10A H840A (catalytically inactive)PromoterAvailable sinceAvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Hygro-PIP-FUCCI
Plasmid#138714PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterhPGKAvailable sinceMay 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-CMV-Blast-PIP-FUCCI
Plasmid#138715PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterCMVAvailable sinceMarch 19, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLenti-PGK-Neo-PIP-FUCCI
Plasmid#118616PurposeLentiviral expression of the cell cycle marker PIP-FUCCIDepositorInsertPIP-FUCCI
UseLentiviralTagsmCherry and mVenusExpressionMammalianMutationPromoterhPGKAvailable sinceMay 20, 2019AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-SNAP25
Plasmid#168031PurposeExpresses eCFP-SNAP25(A141-G206)-mVenus in bacterial cellsDepositorInserteCFP-SNAP25(A141-G206)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pET-CV-Ykt6
Plasmid#168030PurposeExpresses eCFP-Ykt6(Q131-K190)-mVenus in bacterial cellsDepositorInserteCFP-Ykt6(Q131-K190)-mVenus
UseTagsHisx6ExpressionBacterialMutationPromoterAvailable sinceApril 6, 2021AvailabilityAcademic Institutions and Nonprofits only -
pJK511
Plasmid#155384PurposedCas12a oscillator, reverse direction crRNAs. dCas12a (D917A) + PA4-mVenusDepositorInsertsdCas12a (F. novicida)
PA4-mVenus
dCas12a oscillator crRNAs
UseCRISPRTagsExpressionBacterialMutationD917A (nuclease-deactivating)PromoterAvailable sinceDec. 8, 2020AvailabilityAcademic Institutions and Nonprofits only