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Plasmid#219564Purpose3` circularization cassette.To induce Cre-mediated circularization or inversion of a genomic region with concomitant GFP reconstitution and mScarlet expression from the chromosomeDepositorInsert3` CC: Hygro – GFP-N – loxP – mScarlet
UseUnspecifiedTagsExpressionMutationPromoterEF1-aAvailable sinceNov. 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-EFS-Zeo
Plasmid#227261PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Zeo cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-EFS-Blast
Plasmid#227260PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Blast cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-mScarlet-EFS-Puro
Plasmid#227259PurposeEmpty C-terminus donor cassette. Integrate homology arms to target the C-terminus insertion of a mScarlet-EFS-Puro cassetteDepositorArticleTypeEmpty backboneUseCRISPR; Donor cassetteTagsExpressionMammalianMutationPromoterAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
CUP1-∆ssCPY*-mScarletI in pRS315
Plasmid#221159PurposeFluorescent reporter for cytoplasmic aggregates under LEU2 selection markerDepositorInsertCarboxypeptidase Y (PRC1 Budding Yeast)
UseTagsmScarletIExpressionYeastMutationDeletion signal peptide, G255A mutationPromoterAvailable sinceNov. 1, 2024AvailabilityAcademic Institutions and Nonprofits only -
pME V5 mScarlet-I (JDW 1323)
Plasmid#224487PurposeGateway compatible middle entry clone containing V5 tagged m-Scarlet-I (Cytosolic far red fluorescent reporter)DepositorInsertV5-mScarlet-I
UseGateway cloningTagsExpressionMutationPromoterAvailable sinceSept. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pDRF1 mScarletI3-T2A-mTurquoise2
Plasmid#219845Purposeequimolar expression of mScarletI3 and mTurquoise2 in budding yeastDepositorInsertmScarletI3-T2A-mTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceAug. 27, 2024AvailabilityIndustry, Academic Institutions, and Nonprofits -
pSC101-GFPmut2-mScarlet-I TAG
Plasmid#208188Purposenonsense mistranslation, TAG stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TAG*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TGA
Plasmid#208189Purposenonsense mistranslation, TGA stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TGA*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TGATC
Plasmid#208190Purposenonsense mistranslation, TGA stop variant 2DepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TGA*(stop) 7TCT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I A6T
Plasmid#208194Purposeframeshift mistranslation, A6TDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6AAAAAATPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I C3T
Plasmid#208195Purposeframeshift mistranslation, C3TDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationFrameshift insertion 6CCCTPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I 6Trp7Ser
Plasmid#208186Purposemistranslation GFP and mScarlet-I positive control with Trp insertionDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6Trp7SerPromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
pSC101-GFPmut2-mScarlet-I TAA
Plasmid#208187Purposenonsense mistranslation, TAA stopDepositorInsertsGFPmut2
mScarlet-I
UseReporterTagsExpressionBacterialMutationInsertion 6TAA*(stop) 7AGT(Ser)PromoterPtet+dnaK P1Available sinceMarch 25, 2024AvailabilityAcademic Institutions and Nonprofits only -
qTAG-C-Solo-mScarlet-H3C2
Plasmid#207781PurposeDonor template for mScarlet insertion into the C-terminus of the H3C2 locus for nuclei visualization. To be co-transfected with sgRNA plasmid px330-PITCh-H3C2 Addgene #207780DepositorArticleInsertH3C2 Homology Arms flanking a mScarlet Tag (H3C2 Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 29, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
qTAG-N-Solo-mScarlet-ACTB
Plasmid#207750PurposeDonor template for mScarlet insertion into the N-terminus of the ACTB locus for actin visualization. To be co-transfected with sgRNA plasmid px330-PITCh-ACTB Addgene #207748DepositorArticleInsertACTB Homology Arms flanking a mScarlet Tag (ACTB Human)
UseCRISPR; Donor templateTagsExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
S16-ACR1-pcDNA3.1-mScarlet
Plasmid#204963PurposeExpression of S16-ACR1 fused to mScarlet in mammalian cellsDepositorInsertS16-ACR1
UseTagsmScarletExpressionMammalianMutationPromoterCMV (+ enhancer)Available sinceOct. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
CaMKIIa-mScarlet-I-P2A-2M-Qt-FLAG
Plasmid#206956PurposeAAV plasmid for CaMKIIa driven co-expression of mScarlet-I and 2M-Qt-FLAG EMcapsulinsDepositorInsertmScarlet-I, MmMT3, QtEnc
UseAAVTagsFLAGExpressionMutationPromoterAvailable sinceSept. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymTq2-T2A-ymScarletl
Plasmid#205511PurposeExpression of ymTq2-T2A-ymScarletl in yeastDepositorInsertymTq2-T2A-ymScarletl
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pDRF1-GW ymTq2-P2A-ymScarletI
Plasmid#205500PurposeExpression of ymTq2-P2A-ymScarletI in yeastDepositorInsertymTq2-P2A-ymScarletI
UseTagsExpressionYeastMutationPromoterAvailable sinceSept. 13, 2023AvailabilityAcademic Institutions and Nonprofits only