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Plasmid#185912PurposepCMV and pT7 plasmid encoding human codon optimized ABE8e A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8e-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCMV-T7-ABE8.20m-nSpRY-P2A-EGFP (KAC1335)
Plasmid#185917PurposepCMV and pT7 plasmid encoding human codon optimized ABE8.20m A-to-G base editor with nicakse SpRY(D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219Q/N1317R/A1322R/R1333P/R1335Q/T1337R) and P2A-EGFPDepositorInserthuman codon optimized ABE8.20m-nSpRY-P2A-EGFP
UseIn vitro transcription; t7 promoterTagsBPNLS and BPNLS-3xFLAG-P2A-EGFPExpressionMammalianMutationnSpRY=D10A/A61R/L1111R/D1135L/S1136W/G1218K/E1219…PromoterCMV and T7Available sinceJuly 20, 2022AvailabilityAcademic Institutions and Nonprofits only -
pET28a-sfGFP 150TAG
Plasmid#85493PurposepET28a expressing E. coli codon optimized superfolder GFP with TAG stop codon at position 150DepositorInsertsuperfolder GFP 150TAG
UseTagsExpressionBacterialMutation150 TAG (amino acid 150 mutated to stop codon)PromoterT7Available sinceJan. 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pBadcrtEBI_13
Plasmid#63811Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_19
Plasmid#64045Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_27
Plasmid#63806Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_2
Plasmid#63803Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_52
Plasmid#63808Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_37
Plasmid#63807Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_25
Plasmid#63805Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceMay 15, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_14
Plasmid#63804Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceMay 7, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_64
Plasmid#63809Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
LacI_PlacO1_crtEBI_13
Plasmid#63812Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPlaco1Available sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pBad_crtEBI_70
Plasmid#63810Purposeexpresses crtEBI genes for neurosporene productionDepositorInsertcrtEBI operon
UseTagsExpressionBacterialMutationCodon optimized for E.coliPromoterPBADAvailable sinceApril 3, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-DuraPETase
Plasmid#218699PurposeEncodes codon optimized DuraPETase enzyme for bacterial expressionDepositorInsertDuraPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRP935
Plasmid#159302PurposeYeast tagging vector to create gene fusion with yeast codon optimized mAmetrine under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 16, 2020AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-TurboPETase
Plasmid#218693PurposeEncodes codon optimized TurboPETase enzyme for bacterial expressionDepositorInsertTurboPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 22, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-HotPETase
Plasmid#218701PurposeEncodes codon optimized HotPETase enzyme for bacterial expressionDepositorInsertHotPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
pCDB179-DepoPETase
Plasmid#218702PurposeEncodes codon optimized DepoPETase enzyme for bacterial expressionDepositorInsertDepoPETase
UseTags10xHisExpressionBacterialMutationPromoterAvailable sinceMay 21, 2024AvailabilityAcademic Institutions and Nonprofits only -
FRP1999
Plasmid#159308PurposeYeast tagging vector to create gene fusion with yeast codon optimized mCardinal under KanMX selectionDepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceSept. 28, 2020AvailabilityAcademic Institutions and Nonprofits only