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Plasmid#74448PurposeThis plasmid contains human codon optimized sequence of mitoLbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertmitoLbNOX
UseTagsFlag tag with linker and mitochondrial targeting …ExpressionMutationHuman codon optimizedPromoterAvailable sinceJune 3, 2016AvailabilityAcademic Institutions and Nonprofits only -
pUC57-LbNOX
Plasmid#75285PurposeThis plasmid contains human codon optimized sequence of LbNOX which is a water-forming NADH oxidase that can be used to increase NAD+/NADH ratio in mammalian cells.DepositorInsertLbNOX
UseTagsC-terminal FLAG tag with linkerExpressionMutationHuman codon optimizedPromoterAvailable sinceMay 17, 2016AvailabilityAcademic Institutions and Nonprofits only -
pTUB1:OsTIR1-3FLAG, SAG1:CAT
Plasmid#87258PurposeTIR1-3FLAG fusion driven by a TUB1 promoter with a CAT drug selectable marker. The TIR1 CDS from Oryza sativa was codon optimized for T. gondii expression.DepositorInsertsTIR1
CAT
UseToxoplasma expressionTags3FLAGExpressionMutationCodon optimized for Toxoplasma gondii expressionPromoterTgSAG1 361 bp and TgTUB1 2717 bpAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pNPK-NLS-Vika
Plasmid#79969Purposeexpresses human codon-optimized Vika-recombinase in mammalian cellsDepositorInserthuman codon-optimized Vika sequence, NLS tagged
UseTagsNLSExpressionMammalianMutationPromoterPGKAvailable sinceAug. 29, 2016AvailabilityAcademic Institutions and Nonprofits only -
pET24a-ncOGT-FL
Plasmid#190821PurposeFor expression of full-length human ncOGT in E.coli. The ncOGT is 6x His-tagged on its N-terminus and codon-optimized for E.coli.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTags6x HisExpressionBacterialMutationCodon optimized for expression in E.coliPromoterT7Available sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pcDNA3.1-OGT_opt
Plasmid#190858PurposeFor mammalian expression of wild-type full-length human ncOGT. The ncOGT is not epitope-tagged, but is codon-optimized for human cells.DepositorInsertO-GlcNAc Transferase (OGT Human)
UseTagsExpressionMammalianMutationCodon optimized for expression in human cells.PromoterCMVAvailable sinceJan. 9, 2023AvailabilityIndustry, Academic Institutions, and Nonprofits -
pUC18T-mini-Tn7T-Gm-Pc-mTurquoise2
Plasmid#111259Purposemini-Tn7 vector for insertion of mTurquoise2 (codon optimized for P. fluorescens) at bacterial attTn7 siteDepositorInsertmTurquoise2
UseTagsExpressionBacterialMutationmTurquoise2 is codon optimized for expression in …PromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
Cas9 MDC123
Plasmid#59184Purpose2x35S promoting Glycing Max codon optimized Cas9 with bar plant selectionDepositorInsertGlycine Max codon optimized Cas9
UseCRISPRTagsExpressionPlantMutationPromoter2X35SAvailable sinceNov. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
pTrc-trGPPS(CO)-LS
Plasmid#50603PurposepTrc-trGPPS(CO)-LS: A plasmid that has truncated codon optimized geranylpyrophosphate synthase and truncated limonene synthaseDepositorInsertsLimonene Synthase
ApR
LacIq
UseSynthetic BiologyTagsExpressionBacterialMutationtruncated codon optimized geranylpyrophosphate sy…PromoterTrcAvailable sinceJuly 15, 2014AvailabilityAcademic Institutions and Nonprofits only -
pHis-parallel1-NLSH2BCas9
Plasmid#112065PurposeExpression and purification of NLSH2BCas9 for fungal gene editingDepositorInsertNLS of the Fusarium oxysporum histone H2B fused to SpCas9 that was codon optimized fro Aspergillus niger
UseCRISPRTagsHis tagExpressionBacterialMutationCas9 was codon optimized for Aspergillus nigerPromoterpromoter in vectorAvailable sinceJuly 31, 2018AvailabilityAcademic Institutions and Nonprofits only -
G10 Cas9 MDC123
Plasmid#59187PurposeG10 promoting Glycing Max codon optimized Cas9 (N and C terminus NLS) with bar plant selectionDepositorInsertGlycine Max codon optimized Cas9
UseCRISPRTagsExpressionPlantMutationPromoterG10Available sinceDec. 19, 2016AvailabilityAcademic Institutions and Nonprofits only -
pFPV25.1_rpsM_mCerulean3
Plasmid#124904PurposerpsM promoter (S. typhimurium) driving mCerulean3 (codon-optimized for S. typhimurium)DepositorInsertrpsM promoter and mCerulean3
UseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 26, 2019AvailabilityAcademic Institutions and Nonprofits only -
pSJ1458 (pFA6-link-mTurquoise2-CaURA3MX)
Plasmid#86424PurposePCR tagging module with yeast codon optimized mTurquoise2DepositorInsertmTurquoise2
UseTagsExpressionYeastMutationPromoterAvailable sinceApril 24, 2017AvailabilityAcademic Institutions and Nonprofits only -
PE_CO
Plasmid#190335PurposePrime editor (PE2) with codon-optimized Reverse transcriptase for prime editing in mammalian cellsDepositorInsertPE2 with codon-optimized reverse transcriptase
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 21, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAdSh.PGK.Cas9
Plasmid#58253PurposeExpresses human codon-optimized S. pyogenes Cas9 from the human PGK-1 gene promoterDepositorInsertHuman codon-optimized S. pyogenes Cas9
UseAdenoviral and CRISPRTagsExpressionMammalianMutationPromoterAvailable sinceSept. 5, 2014AvailabilityAcademic Institutions and Nonprofits only -
pJIT163-2NLSCas9
Plasmid#53064PurposeExpression of rice codon-optimized Cas9 in plant cellsDepositorInsertCas9
UsePlant expressionTagsExpressionMutationRice codon optimizedPromoter2x35SAvailable sinceAug. 21, 2014AvailabilityAcademic Institutions and Nonprofits only -
HBT_pcoCASphi_version2
Plasmid#197932PurposeCloning vector with Arabidopsis codon optimized Casphi-2 protein, with SV40 NLS at the C-terminalDepositorInsertpcoCasphi-2
UseCRISPRTags2xNLS-2xFLAGExpressionPlantMutationPromoter35SPPDK promoterAvailable sinceMarch 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSW002-Pc-mRuby2
Plasmid#111250PurposeBroad host-range bacterial expression vector with constitutive Pc promoter; for tagging bacteria with mRuby2 (codon optimized for P. fluorescens)DepositorInsertmRuby2
UseTagsExpressionBacterialMutationmRuby2 is codon optimized for expression in P. fl…PromoterPcAvailable sinceAug. 9, 2018AvailabilityAcademic Institutions and Nonprofits only -
pHC01
Plasmid#106900PurposeRatiometric gas biosensor plasmid for 3-oxo-C12-HSL sensingDepositorInsertsLasR
Methyl Halide Transferase
Ethylene Forming Enzyme
Red Fluorescent Protein
UseTagsExpressionBacterialMutationPromoterAvailable sinceMarch 17, 2018AvailabilityAcademic Institutions and Nonprofits only -
pJ204-loxP-100RPU.2-coEPO-BGHpA-cHS4-100RPU.2-coEPO-lox2272
Plasmid#154829PurposeDonor plasmid for Cre/lox-based recombinase-mediated cassette exchange (RMCE), expressing two copies of erythropoietinDepositorInsertscoEPO
coEPO
UseCre/LoxTagsExpressionMammalianMutationCodon-optimized (Cricetulus griseus)Promoter100RPU.2Available sinceAug. 25, 2020AvailabilityAcademic Institutions and Nonprofits only