This G protein alpha-q construct contains internal insertions of GFP and the EE epitope

Expression of a sensor for synthetic sequence 1 (EK0208) with three MS2 sequences in the 3' UTR in mammalian cells. Compared to EK0285, also has additional restriction sites around the sensor sequence. Recommended for the basis for cloning new sensors for use with MCP-ADAR(DD). HindIII & MluI can be used to swap out marker (mCherry). (KpnI or BamHI) and (BshTI or BcuI) can be used to swap out sensor sequence (s70587). XhoI and ApaI can be used to swap out output (EGFP).

This G protein alpha-q construct contains internal insertions of CFP and the EE epitope

Panel of cancer alleles that have undergone basic, functional screening; full-length, human clones (454 mutant and 177 corresponding wild-type) in the Gateway cloning system

Combinatorial assembly method that combines Golden Gate (Type IIs endonucleases) cloning with Multisite Gateway (recombination) cloning to construct complex plasmids in an efficient and modular way

190 human kinases and kinase-related open reading frames (ORFs) cloned into a retroviral vector which adds a myristoylation sequence and flag-epitope tag to each ORF.

556 distinct human kinases and kinase-related protein ORFs in a pDONR-223 Entry Gateway vector. All clones are clonal isolates.