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Plasmid#117536PurposeLevel0 Golden Gate part: c-terminal tag, YFPDepositorInsertc-term tag YFP
UseSynthetic BiologyTagsExpressionMutationG65S, L68V, A72S, Y203T, L231HPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pAFZifPZ431
Plasmid#185849PurposeExpressing artificial trans-activator in Saccharomyces cerevisiaeDepositorInsertPHAC1>ZifPZ43>MED3>PABF1
UseTagsExpressionYeastMutationNonePromoterAvailable sinceSept. 1, 2022AvailabilityAcademic Institutions and Nonprofits only -
mCh-LaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
Plasmid#165030PurposeExpress cpFRB2-based GFP specific Chessbody (chemically switchable split nanobody); visualized by mCherryDepositorInsertLaGFP(N)-cpFRB2-T2A-FKBP-LaGFP(C)
UseTagsmCherryExpressionBacterial and MammalianMutationPromoterT7Available sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
pEPOR0SP0020
Plasmid#117531PurposeLevel0 Golden Gate part: CDS, SaCas9DepositorInsertSaCas9 (with stop codon)
UseCRISPRTagsExpressionMutationPromoterAvailable sinceNov. 13, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGWB501NL3F10H
Plasmid#141286PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, HygR for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceFeb. 16, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCfB2189
Plasmid#67532PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlLEU2 marker, integration into S. cerevisiae X-3 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3_BtKY72-Spike
Plasmid#190084Purposeexpress BtKY72 spikeDepositorInsertBtKY72-CoV S bat
UseTagsRhodopsin C9ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 8, 2022AvailabilityAcademic Institutions and Nonprofits only -
pGWB601NL3F10H
Plasmid#141287PurposeNo promoter, C-terminal NLUC:3xFlag:10xHis tag, attR1-Cmr-ccdB-attR2-NLUC:3xFlag:3xHis-TNOS (modified pGWB401, pPZP backbone, SpcR for bacteria, BASTA R for plant)DepositorTypeEmpty backboneUseLuciferase; GatewayTagsFLAG (x3), HIS (x10), and NLUCExpressionPlantMutationPromoterAvailable sinceJuly 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCS2 xRor2-mCherry
Plasmid#67162Purposemammalian expression / in vitro transcriptionDepositorInsertTyrosine-protein kinase transmembrane receptor ROR2 (ror2 Frog)
UseIn vitro transcriptionTagsmCherryExpressionMammalianMutationPromoterAvailable sinceJuly 16, 2015AvailabilityAcademic Institutions and Nonprofits only -
CP2-TCC
Plasmid#179679PurposeComplementary plasmidDepositorInsert[sd2] T7 RNAP-TCC linker-degron
UseTagsExpressionBacterialMutationPromoterAvailable sinceApril 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI2
Plasmid#176156Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-2DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pCfB2188
Plasmid#67296PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked KlURA3 marker, integration into S. cerevisiae X-2 chromosomal location, USER site for cloning, amp resistance.DepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GR-ICUE2
Plasmid#173017PurposeImproved green-red FRET based cAMP indicator.DepositorInsertGR-ICUE2
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceSept. 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTorPE-G-GECO1.2
Plasmid#32468DepositorArticleInsertG-GECO1.2
UseLab constructedTags6 His tag and Modified TorA MNNNDLFQASRRRFLAQLG[G…ExpressionBacterialMutationGCaMP3 K69E/N77Y/D86G/K119I/L173Q/D260G/S404G/E43…PromoterAvailable sinceSept. 29, 2011AvailabilityAcademic Institutions and Nonprofits only -
pCfB2337
Plasmid#67555PurposeEasyClone system-based yeast integrative vector carrying loxP-flanked hphMX marker, integration into S. cerevisiae XII-5 chromosomal location, USER site for cloning, amp resistanceDepositorTypeEmpty backboneUseCre/Lox; IntegrativeTagsExpressionYeastMutationPromoterAvailable sinceMarch 10, 2016AvailabilityAcademic Institutions and Nonprofits only -
pMC-X4
Plasmid#176154Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site X-4DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XII1
Plasmid#176159Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-1DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XI3
Plasmid#176157Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XI-3DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pMC-XII5
Plasmid#176161Purposepreassembled yeast MoClo level-2 backbone with GFP dropout for genomic integration in site XII-5DepositorTypeEmpty backboneUseTagsExpressionYeastMutationPromoterAvailable sinceFeb. 4, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBT271.359
Plasmid#127845Purposeclone A3A CBEs by SapI Golden GateDepositorInsertA3A
UseTagsExpressionMutationPromoterAvailable sinceJuly 25, 2019AvailabilityAcademic Institutions and Nonprofits only