-
Plasmid#199478PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_1_mTagBFP2-T2A-sGFP11x7_synCoTC
Plasmid#199479PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSA_2_mTagBFP2-T2A-sGFP11x7_synCoTC
Plasmid#199480PurposeSite directed zebrafish mutagenesis. sgRNA GAAGATCGGCCACTACATTCDepositorArticleInsertmTagBFP2-T2A-sGFP11x7
UseCRISPRTagsExpressionMutationPromoterAvailable sinceJuly 12, 2023AvailabilityAcademic Institutions and Nonprofits only -
WT-short-UBASH3A-GFP11
Plasmid#195385PurposeMammalian expression of human wild-type short-form UBASH3A with GFP(11) tagDepositorInsertUBASH3A (UBASH3A Human)
UseTagsGFP(11)ExpressionMammalianMutationPromoterAvailable sinceMarch 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
pET303-ColE1-T-P110A
Plasmid#180237PurposeExpresses T domain of Colicin E1 (residues 1-190) with proline to alanine mutation at residue 110DepositorInsertColicin E1
UseTags6x His-tagExpressionBacterialMutationTruncation of ColE1 containing T domain of ColE1 …PromoterT7Available sinceAug. 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1(+)-GFP11-RhoA
Plasmid#181860PurposePart of FluoSTEP-RhoA biosensor; co-express with cpPKN-mRuby2-GFP1-10 (Addgene #181859).DepositorInsertGFP11-RhoA (RHOA Human)
UseTagsGFP11ExpressionMammalianMutationPromoterCMVAvailable sinceMay 26, 2022AvailabilityAcademic Institutions and Nonprofits only -
pAGM9121_TEV-His-GFP11
Plasmid#153510PurposeTEV-His-GFP11 C-terminal Tag Donor PlasmidDepositorInsertTEV-His-GFP11
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceJan. 28, 2021AvailabilityAcademic Institutions and Nonprofits only -
YCe1889 HC_Kan_SV40-polyA_p11
Plasmid#100678Purposepart designed to occupy position 11 of EMMA. Functional category: polyadenylation signal sequenceDepositorInsertSV40-polyA
UseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
YCe1703 HC_Kan_RFP-p11
Plasmid#100621PurposeEMMA toolkit part-entry vector position 11DepositorTypeEmpty backboneUseSynthetic BiologyTagsExpressionMutationPromoterAvailable sinceNov. 17, 2017AvailabilityAcademic Institutions and Nonprofits only -
pET11+PCNA3-CYP119
Plasmid#66136PurposeExpresses the wild type of CYP119 fused to Sulfolobus solfataricus PCNA3DepositorInsertCYP119 (cyp119 )
UseTagsSulfolobus solfataricus PCNA3ExpressionBacterialMutationPromoterT7 promoterAvailable sinceJuly 9, 2015AvailabilityAcademic Institutions and Nonprofits only -
GFP-fly Arp11
Plasmid#51399Purposeexpression of full-length Drosophila Arp11 in mammalian cellsDepositorInsertArp11 (Arp10 Fly)
UseTagsEGFPExpressionMammalianMutationPromoterCMVAvailable sinceMarch 13, 2014AvailabilityAcademic Institutions and Nonprofits only -
pQCXIP-BSR-GFP11
Plasmid#68716PurposeSplit GFP assayDepositorInsertbsr
UseRetroviralTagsFLAG and GFP11ExpressionMammalianMutationPromoterAvailable sinceJan. 20, 2016AvailabilityAcademic Institutions and Nonprofits only -
pmGFP-ADAR1-p110
Plasmid#117928PurposeADAR1-p110 expression plasmid.DepositorInsertADAR1-p110 (ADAR Human)
UseTagsmyc-GFPExpressionMammalianMutationPromoterCMVAvailable sinceDec. 4, 2018AvailabilityAcademic Institutions and Nonprofits only -
p11d-tRPA(123)
Plasmid#102613PurposeExpression of human RPA in E. coliDepositorUseTagsExpressionBacterialMutationPromoterT7Available sinceNov. 7, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDEST-EGFP_Hu_p115-RhoGEF(WT)
Plasmid#136337PurposeMammalian expression of human p115-RhoGEF with N-terminal EGFPDepositorInsertARHGEF1 (ARHGEF1 Human)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA ZIP-GFP11
Plasmid#182242PurposeTripartite split-GFP mammalian. Express leucine zipper with GFP11DepositorInsertZIP-GFP11
UseTagssplit-GFP11 tripartiteExpressionMammalianMutationPromoterCMVAvailable sinceApril 15, 2022AvailabilityAcademic Institutions and Nonprofits only -
pBS-KS-attB1-2-PT-SA-SD-1-GFP11x7
Plasmid#172067PurposeProtein trap plasmid for inserting GFP11x7 into a phase 1 coding intronDepositorInsertGFP11x7
UseTagsExpressionInsectMutationPromoterAvailable sinceOct. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3-GFP11(x7)-Actin
Plasmid#181967PurposeHuman beta actin tagged with 7 copies of GFP11.DepositorInsertGFP11(x7)-Actin (ACTB )
UseTags6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…ExpressionMammalianMutationPromoterCMVAvailable sinceJune 3, 2022AvailabilityAcademic Institutions and Nonprofits only -
p11-LacY-wtx1
Plasmid#69056PurposeThe reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.DepositorUseTagsA177CExpressionMutationPromoterpBAD and pLacAvailable sinceSept. 30, 2015AvailabilityAcademic Institutions and Nonprofits only -
Flag-GFP11-pDEST-C
Plasmid#118367PurposeBimolecular Fluorescence Complimentation (BiFC) assay. Gateway compatible C-terminal vector with BiFC-tag (GFP11-Flag) to screen interaction partners.DepositorTypeEmpty backboneUseDestinationTagsGFP11-FLAGExpressionMammalianMutationPromoterAvailable sinceMay 1, 2019AvailabilityAcademic Institutions and Nonprofits only