Addgene

We narrowed to 2,130 results for: CO

Showing: 341 - 360 of 2130 results

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pTAG6E

Plasmid

#176870

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_6E

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pMC-1-CmYLCV-6

Plasmid

#176872

Purpose

MoClo Level 0 plasmid, contains CmYLCV-6 promoter

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

CmYLCV

Use

Tags

Expression

Plant

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG2

Plasmid

#176864

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_2

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG3

Plasmid

#176866

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_3

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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pTAG4

Plasmid

#176867

Purpose

First sgRNA in a tRNA separated array of sgRNAs

Depositor

Daniel Voytas

Article

Cody et al Nat Protoc. 2022 Oct 17. pii: 10.1038/s4

Insert

sgRNA_4

Use

CRISPR

Tags

Expression

Mutation

WT

Promoter

Available since

May 9, 2022

Availability

Academic Institutions and Nonprofits only

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kank1b_pJet1.2

Plasmid

#121979

Purpose

for generating kank1b riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al Sci Rep. 2016 Jun 13;6:27816. doi: 10.10

Insert

kank1b (si:dkey-226m8.9 Zebrafish)

Use

Cloning vector

Tags

Expression

Mutation

Promoter

T7

Available since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV5-OCT-HA-eGFP

Plasmid

#67484

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pCMV3-OCT-HA-eGFP

Plasmid

#67482

Purpose

Targets HA-eGFP to mitochondrial matrix

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

OCT-HA-eGFP

Use

Tags

Expression

Mammalian

Mutation

Promoter

attenuated CMV

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDEST14-SARA

Plasmid

#67389

Purpose

Expression of native hSara in E. coli

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

SARA (SAR1B Human)

Use

Tags

Expression

Bacterial

Mutation

Promoter

T7

Available since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pAJ12

Plasmid

#14914

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Use

Tags

GFP(S65C)

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAJ14

Plasmid

#14916

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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pAJ15

Plasmid

#14917

Depositor

James Lok

Article

Junio et al Exp Parasitol. 2008 Feb . 118(2):253-65.

Insert

multiple cloning site 3

Use

Tags

Expression

Worm

Mutation

Promoter

Available since

July 18, 2007

Availability

Academic Institutions and Nonprofits only

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AAVS1_Puro_hPGK1_EGFP_Donor

Plasmid

#178088

Purpose

HDR donor plasmid to introduce the EGFP cassette to AAVS1 using AAVS1 T2 sgRNA.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

EGFP

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Human PGK1

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO V5 HSPH1

Plasmid

#19506

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2008 Aug 7. ():.

Insert

heat shock 105kDa/110kDa protein 1 (HSPH1 Human)

Use

Tags

V5

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 6, 2008

Availability

Academic Institutions and Nonprofits only

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pAD/CMV/V5-DEST-Cdc42-2G

Plasmid

#68812

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for Adenovirus production)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Adenoviral

Tags

His and Myc

Expression

Mammalian

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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R4227X-F

Plasmid

#21371

Depositor

Gregory Germino

Article

Hanaoka et al Nature. . 408(6815):990-4.

Insert

autosomal dominant polycystic kidney disease type I (PKD1 Human)

Use

Tags

FLAG (following D4226)

Expression

Mammalian

Mutation

Changed R4227 to stop codon

Promoter

Available since

Jan. 22, 2010

Availability

Academic Institutions and Nonprofits only
pTriEx4-Cdc42-2G

Plasmid

#68814

Purpose

FRET-based biosensor reporting on endogenous Cdc42 activation (for expression in E. coli, insect and vertebrate cells)

Depositor

Olivier Pertz

Article

Martin et al Sci Rep. 2016 Feb 25;6:21901. doi: 10.10

Insert

Cdc42 activation reporter

Use

Tags

His and Myc

Expression

Bacterial, Insect, and Mamm…

Mutation

Promoter

CMV

Available since

March 1, 2016

Availability

Academic Institutions and Nonprofits only

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pH7m34GW-35S:SNACS S785A

Plasmid

#203577

Purpose

Plant SnRK2S30A kinase activity FRET sensor in plant expression vector

Depositor

Julian Schroeder

Article

Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

Insert

SNACS S785A

Use

Tags

StrepII

Expression

Plant

Mutation

S785A

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pH7m34GW-35S:SNACS

Plasmid

#203576

Purpose

Plant SnRK2 kinase activity FRET sensor in plant expression vector

Depositor

Julian Schroeder

Article

Zhang et al eLife. 2020 May 28;9:e56351. doi: 10.755

Insert

SNACS

Use

Tags

StrepII

Expression

Plant

Mutation

none

Promoter

Available since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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NPM1_mNeonGreen_Donor

Plasmid

#178093

Purpose

HDR donor plasmid to tag NPM1 with mNeonGreen using eSpCas9(1.1)_No_FLAG_NPM1_G6.

Depositor

Yannick Doyon

Article

Levesque et al Nat Commun. 2022 Oct 7;13(1):5909. doi:

Insert

NPM1-mNeonGreen HDR donor

Use

CRISPR

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Dec. 14, 2021

Availability

Academic Institutions and Nonprofits only

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